The Effect of GSPs-TACE on the Growth of Liver Transplant Tumors in Rabbits

Chunhai HAO

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Chunhai HAO. The Effect of GSPs-TACE on the Growth of Liver Transplant Tumors in Rabbits[J]. Journal of Kunming Medical University.

Citation:

Chunhai HAO. The Effect of GSPs-TACE on the Growth of Liver Transplant Tumors in Rabbits[J]. Journal of Kunming Medical University.

Chunhai HAO. The Effect of GSPs-TACE on the Growth of Liver Transplant Tumors in Rabbits[J]. Journal of Kunming Medical University.

Citation:

Chunhai HAO. The Effect of GSPs-TACE on the Growth of Liver Transplant Tumors in Rabbits[J]. Journal of Kunming Medical University.

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The Effect of GSPs-TACE on the Growth of Liver Transplant Tumors in Rabbits

Chunhai HAO

Department of Hepatobiliary Surgery，Tangshan People's Hospital，Tangshan Hebei 063000，China

Received Date: 2024-08-26

Abstract

Abstract

Objective To investigate the effect of gelatin sponge particle chemotherapy combined with hepatic artery chemoembolization on the growth of rabbit liver transplant tumors. Method Sixty New Zealand white rabbits were randomly divided into a model control group and gelatin sponge particles （GSPs）- Transcatheterarterial chemoembolization. The TACE group, the epirubicin-TACE group and the GSPs+ epirubicin-TACE group （n = 15）. VX2 cell liver cancer models were established in all experimental rabbits. After the operation, in the GSPS-TACE group, GSPs were perfused during TACE. In the epirubicin-TACE group, epirubicin solution （1mg/mL） was perfused. In the GSPs+ epirubicin-TACE group, a mixture of epirubicin solution and GSPs was used for embolization. Computed tomography was used to detect the tumor volume of experimental rabbits in each group. Hematoxylin-eosin staining was used to observe the pathological morphology. The apoptosis rate of tumor cells was detected by Terminal Deoxynucleotidyl Transferase-Mediated dUTPNick End Labeling （TUNEL） staining; Immunohistochemical detection of positive expression of vascular endothelial growth factor （VEGF）; Western blotting was used to detect B-cell lymphoma 2 （Bcl-2）, Bcl2-associated X protein （Bax）, and cysteine-aspartic acid Protein expression of protease-3, caspase-3. Results Compared with the model control group, the tumor volume, the proportion of intrahepatic metastasis, the proportion of intra-abdominal metastasis, VEGF, and Bcl-2 in the GSPs-TACE group were all decreased （P < 0.05）, while the apoptosis rate of tumor cells, Bax, Bcl-2/Bax, and caspase-3 were all increased （P < 0.05）. There was no statistically significant difference between the GSPS-TACE group and the epirubicin-TACE group （P > 0.05）. Compared with the epirubicin-TACE group, the tumor volume, the proportion of intrahepatic metastasis, the proportion of intra-abdominal metastasis, VEGF, and Bcl-2 in the GSPs+ epirubicin-TACE group were all decreased （P < 0.05）. The apoptosis rate of tumor cells, Bax, Bcl-2/Bax, and caspase-3 all increased （P < 0.05）. Conclusion Gelatin sponge microparticle chemotherapy combined with hepatic artery chemoembolization inhibits the growth of rabbit liver transplant tumors.
- Liver transplant tumor,
- Gelatin sponge particles,
- Hepatic artery chemoembolization,
- Apoptosis,
- Vascular endothelial growth factor

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References(22)

References

[1]	Zheng J, Wang S, Xia L, et al. Hepatocellular carcinoma: signaling pathways and therapeutic advances[J]. Signal Transduct Target Ther., 2025, 10(1): 35. doi: 10.1038/s41392-024-02075-w
[2]	Xia C, Dong X, Li H, et al. Cancer statistics in china and united states, 2022: profiles, trends, and determinants[J]. Chin Med J (Engl), 2022, 135(5): 584-90. doi: 10.1097/CM9.0000000000002108
[3]	Sung H, Ferlay J, Siegei R L, et al. Global cancer statistics 2020: globocan estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2021, 71(3): 209-49. doi: 10.3410/f.739487650.793592245
[4]	Li JJ, Wang JH, Tian T, et al. The liver microenvironment orchestrates FGL1-mediated immune escape and progression of metastatic colorectal cancer[J]. Nat Commun, 2023, 14(1): 6690. doi: 10.1038/s41467-023-42332-0
[5]	Jin Z C, Zhang Q, Zhu HD, et al. [Research progress of adjuvant TACE therapy for liver cancer after radical resection][J]. Zhonghua Gan Zang Bing Za Zhi, 2022, 30(3): 340-344.
[6]	Cho Y, Choi JW, Kwon H, et al. Research committee of the korean liver cancer association. transarterial chemoembolization for hepatocellular carcinoma: 2023 expert consensus-based practical recommendations of the korean liver cancer association[J]. Korean J Radiol, 2023, 24(7): 606-625. doi: 10.3348/kjr.2023.0385
[7]	Gong Y, Zhang J, Sun B. Microwave ablation versus bleomycin-lipiodol emulsion with gelatin sponge embolization for hepatic hemangioma: efficacy and recovery outcomes in a retrospective cohort study[J]. Am J Cancer Res., 2025, 15(5): 2319-2331. doi: 10.62347/KYOM6165
[8]	吴越, 梁长虹, 刘昕, 等. 兔VX2肝癌模型不同生长期的CT灌注成像与组织学对照研究[J]. 第四军医大学学报, 2009, 30(24): 3009-3013. doi: 10.3321/j.issn:1000-2790.2009.24.030
[9]	高文慧, 陈志军, 简晓顺, 等. 经肝动脉灌注人血白蛋白-索拉非尼纳米粒联合明胶海绵颗粒栓塞治疗在兔VX2肝癌模型中的实验研究[J]. 中国医院药学杂志, 2023, 43(21): 2373-2378.
[10]	Vogl TJ, Lahrsow M. The role of conventional TACE (cTACE) and DEBIRI-TACE in colorectal cancer liver metastases[J]. Cancers (Basel), 2022, 14(6): 1503. doi: 10.3390/cancers14061503
[11]	慕华, 王亚兵. 表柔比星联合奥沙利铂和氟尿嘧啶经导管动脉栓塞化疗用于原发性肝癌患者的效果[J]. 中国民康医学, 2024, 36(1): 42-44.
[12]	李军磊. 雷替曲塞与表柔比星TACE治疗原发性肝癌患者的疗效及安全性对比[J]. 基层医学论坛, 2022, 26(32): 46-48. doi: 10.19435/j.1672-1721.2022.32.016
[13]	Sato H, Sonomura T, Koike M, et al. Comparison of soluble gelatin sponge particles and tris-acryl gelatin microspheres for bariatric arterial embolization in swine[J]. J Vasc Interv Radiol, 2022, 33(9): 1097-1100. doi: 10.1016/j.jvir.2022.05.025
[14]	Fan W, Zhu B, Chen S, et al. Survival in patients with recurrent intermediate-stage hepatocellular carcinoma: sorafenib plus tace vs tace alone randomized clinical trial[J]. JAMA Oncol., 2024, 10(8): 1047-1054. doi: 10.1001/jamaoncol.2024.1831
[15]	Zeng J, Wang Y, Wang H, et al. Comparison of 8spheres polyvinyl alcohol microsphere and gelatin sponge particle efficacy for transcatheter arterial chemoembolization in stages A to B patients with hepatocellular carcinoma[J]. J Cancer Res Ther., 2022, 18(7): 1981-1987. doi: 10.4103/jcrt.jcrt_736_22
[16]	Tan XP, He Y, Yang J, et al. Blockade of NMT1 enzymatic activity inhibits N-myristoylation of VILIP3 protein and suppresses liver cancer progression[J]. Signal Transduct Target Ther, 2023, 8(1): 14. doi: 10.1038/s41392-022-01248-9
[17]	Zhao S, Zhang Y, Lu X, et al. CDC20 regulates the cell proliferation and radiosensitivity of P53 mutant HCC cells through the Bcl-2/Bax pathway[J]. Int J Biol Sci, 2021, 17(13): 3608-3621. doi: 10.7150/ijbs.64003
[18]	Wang S, Chen X, Zhang X, et al. Pro-apoptotic gene BAX is a pan-cancer predictive biomarker for prognosis and immunotherapy efficacy[J]. Aging (Albany NY)., 2024, 16(14): 11289-11317. doi: 10.18632/aging.206003
[19]	Xiang L, Li Y, Gu X, et al. Nucleolin recognizing silica nanoparticles inhibit cell proliferation by activating the Bax/Bcl-2/caspase-3 signalling pathway to induce apoptosis in liver cancer[J]. Front Pharmacol, 2023, 9(14): 1117052. doi: 10.3389/fphar.2023.1117052
[20]	Mok EHK, Leung CON, Zhou L, et al. Caspase-3-induced activation of SREBP2 drives drug resistance via promotion of cholesterol biosynthesis in hepatocellular carcinoma[J]. Cancer Res, 2022, 82(17): 3102-3115. doi: 10.1158/0008-5472.CAN-21-2934
[21]	胡灵溪, 李妹, 付艺伟, 等. 血清血管内皮生长因子对肝动脉化疗栓塞单独/联合靶免治疗在中晚期肝癌临床疗效中的评估价值[J]. 中国全科医学, 2024, 27(32): 4033-4039
[22]	何慧洲. 肝癌TACE术后缺氧对肿瘤细胞糖酵解代谢及血管新生影响的研究[D]. 贵州医科大学, 2023.

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