Effects of p16 on the Cell Cycle and Fatty Acid Metabolism of Gallbladder Cancer Cells

Yongli MO; Xiaoping WEI; Henghai YU; Rui LIU

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Yongli MO, Xiaoping WEI, Henghai YU, Rui LIU. Effects of p16 on the Cell Cycle and Fatty Acid Metabolism of Gallbladder Cancer Cells[J]. Journal of Kunming Medical University.

Citation:

Yongli MO, Xiaoping WEI, Henghai YU, Rui LIU. Effects of p16 on the Cell Cycle and Fatty Acid Metabolism of Gallbladder Cancer Cells[J]. Journal of Kunming Medical University.

Yongli MO, Xiaoping WEI, Henghai YU, Rui LIU. Effects of p16 on the Cell Cycle and Fatty Acid Metabolism of Gallbladder Cancer Cells[J]. Journal of Kunming Medical University.

Citation:

Yongli MO, Xiaoping WEI, Henghai YU, Rui LIU. Effects of p16 on the Cell Cycle and Fatty Acid Metabolism of Gallbladder Cancer Cells[J]. Journal of Kunming Medical University.

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Effects of p16 on the Cell Cycle and Fatty Acid Metabolism of Gallbladder Cancer Cells

Department of Hepatopancreatobiliary Surgery，The Second Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101，China

Abstract

Abstract

Objective To verify the expression pattern of p16 at the clinical and cellular levels, and further explore the effects of p16 on the cell cycle and fatty acid metabolism of human gallbladder cancer （GBC） cells. Methods Thirty specimens of gallstones with cholecystitis and thirty GBC specimens were collected. Quantitative fluorescence PCR （QPCR） was used to detect p16 mRNA levels in GBC samples and cholecystitis samples. Immunohistochemical staining was performed to detect p16 protein expression levels in GBC samples and cholecystitis samples. CCK8 assay was used to detect the proliferative capacity of stably transfected cell lines. Flow cytometry was employed to detect apoptosis and cell cycle progression in tumor cells. QPCR was used to detect the expression of peroxisome-related genes, mitochondrial oxidation-related genes, and fatty acid synthesis-related genes in cancer cells with low p16 expression. Results The expression of p16 mRNA and p16 protein in GBC was significantly higher than that in chronic cholecystitis （P < 0.05）. After p16 downregulation, the apoptosis rate of tumor cells decreased, the proportion of cells in G0/G1 phase decreased, the S phase proportion increased, and cell division accelerated, with extremely significant differences （P < 0.0001）. After p16 downregulation, most related genes in GBC were significantly upregulated, with statistically significant differences （P < 0.05）. Conclusions p16 is significantly highly expressed in GBC. p16 can inhibit the progression of GBC cell cycle. p16 may affect the occurrence and development of GBC cells through regulating the pathway of fatty acid metabolism.
- p16,
- Gallbladder cancer,
- Cell cycle,
- Fatty acid metabolism,
- Tumor

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References(30)

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