Optimization and Practice of LD50 Experimental Teaching Based on An Alcohol Model

Lan GAO; Yuanhang YANG; Kaixuan QIN; Lei ZHANG

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Lan GAO, Yuanhang YANG, Kaixuan QIN, Lei ZHANG. Optimization and Practice of LD50 Experimental Teaching Based on An Alcohol Model[J]. Journal of Kunming Medical University.

Citation:

Lan GAO, Yuanhang YANG, Kaixuan QIN, Lei ZHANG. Optimization and Practice of LD50 Experimental Teaching Based on An Alcohol Model[J]. Journal of Kunming Medical University.

Lan GAO, Yuanhang YANG, Kaixuan QIN, Lei ZHANG. Optimization and Practice of LD50 Experimental Teaching Based on An Alcohol Model[J]. Journal of Kunming Medical University.

Citation:

Lan GAO, Yuanhang YANG, Kaixuan QIN, Lei ZHANG. Optimization and Practice of LD50 Experimental Teaching Based on An Alcohol Model[J]. Journal of Kunming Medical University.

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Optimization and Practice of LD50 Experimental Teaching Based on An Alcohol Model

Department of Toxicology，School of Public Health，Anhui Medical University，Hefei Anhui 230032，China

Received Date: 2025-07-16
Available Online: 2026-01-13

Abstract

Abstract

Objective To explore the feasibility, safety, and teaching equivalence of substituting ethanol （alcohol） for highly toxic test substances in experimental teaching. Methods Undergraduate students majoring in preventive medicine and related disciplines from the classes of 2019—2022 were enrolled. An oral gavage LD₅₀ experiment with ethanol was conducted using Kunming mice in the course Fundamentals of Toxicology Experiment. Five dose groups （5683～23670 mg/kg） were designed, and toxic symptoms and mortality rates within 1 hour were observed. The LD₅₀ and 95% confidence interval were calculated using the modified Kärber method, and teaching safety and sex differences were assessed. Results The mean LD₅₀ of ethanol was 15,439 mg/kg （range: 10,046～22,039 mg/kg）, which was significantly higher than the literature value （3450 mg/kg）, primarily due to the shortened 1-hour observation period. Experimental data were classified and analyzed according to different majors, academic years, and sex of test animals. No statistically significant differences were found in ethanol LD₅₀ values between different majors and academic years （P > 0.05）, nor between female and male mice （P > 0.05）. Experimental data Ethanol demonstrated a clear dose-response relationship, readily observable toxic symptoms, and high safety. Additionally, teaching costs were reduced, operational efficiency improved, and students' experimental experience was enhanced. Conclusion Ethanol serves as a safe, economical, and easy-to-operate test substance for LD₅₀ experiments that fully meets core teaching objectives and complies with the "3Rs" principle （particularly the replacement principle）, demonstrating significant value for promotion.
- LD₅₀,
- Toxicology experimental teaching,
- Alcohol,
- Mouse

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References(22)

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