Exploring Key Mitochondrial Autophagy Genes in Schizophrenia through Integrated Bioinformatics Approaches

Kun LIAN; Yongmei LI; Chenglong SHI; Yilan CHEN; Lei ZHANG; Wei YANG; Xiufeng XU

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Kun LIAN, Yongmei LI, Chenglong SHI, Yilan CHEN, Lei ZHANG, Wei YANG, Xiufeng XU. Exploring Key Mitochondrial Autophagy Genes in Schizophrenia through Integrated Bioinformatics Approaches[J]. Journal of Kunming Medical University.

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Exploring Key Mitochondrial Autophagy Genes in Schizophrenia through Integrated Bioinformatics Approaches

1.
Department of Neurosurgery
2.
Department of Rehabilitation Medicine，The Second Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101
3.
Department of Psychiatry，The Second People's Hospital of Yuxi/Yuxi Hospital Affiliated to Kunming University of Science and Technology，Yuxi Yunnan 653100
4.
Department of Psychiatry，The First Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650000，China

Received Date: 2024-09-30

Abstract

Abstract

Objective To utilize single-cell and peripheral blood transcriptomic data from 3D brain organoids, combined with machine learning, to analyze the role of mitochondrial autophagy genes in schizophrenia （SCZ）. Methods By integrating two machine learning algorithms, we identified differentially expressed mitochondrial autophagy-related genes between schizophrenia patients and healthy controls using peripheral blood RNA sequencing data. The relationship between mitophagy gene, immune cells and inflammatory factors was further explored. Comprehensive single-cell analysis was used to explore the signaling pathways and specific transcription factors based on mitophagy genes. Results Using machine learning, seven key mitophagy genes expressed in schizophrenia patients were identified. Based on Mitoscore analysis, at the single-cell level, neurons with high mitochondrial autophagy activity （Mitohigh_Neuron） formed new interactions with endothelial cells via the SPP1 signaling pathway. Conclusion This study identified two subtypes of mitophagy and seven key mitophagy genes in schizophrenia, providing new insights into the pathogenesis of the disease.
- Schizophrenia,
- Mitochondrial autophagy,
- Neurons,
- Single-cell sequencing,
- Machine learning

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References(27)

References

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