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Yuhong NIU, Xiaomin KANG. Differential Gene Expression Analysis and Functional Prediction of Decidual Myeloid-Derived Suppressor Cells in Patients with Unexplained Recurrent Abortion[J]. Journal of Kunming Medical University.
Citation: Yuhong NIU, Xiaomin KANG. Differential Gene Expression Analysis and Functional Prediction of Decidual Myeloid-Derived Suppressor Cells in Patients with Unexplained Recurrent Abortion[J]. Journal of Kunming Medical University.
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Differential Gene Expression Analysis and Functional Prediction of Decidual Myeloid-Derived Suppressor Cells in Patients with Unexplained Recurrent Abortion

  • 1.

    Medical School of Kunming University of Science and Technology，Kunming Yunnan 650500

  • 2.

    Department of Reproductive Medicine，The First People's Hospital of Yunnan Province，Kunming Yunnan 650034，China

  • Received Date: 2024-06-08
    Available Online: 2024-12-29
    Abstract
  •   Objective  To investigate the differential gene expression and functional prediction of decidual myeloid‐derived suppressor cells （MDSCs） in patients with unexplained recurrent abortion （URSA）.   Methods  Decidual tissues were collected from three pregnant women undergoing early pregnancy termination and three URSA patients at the First People's Hospital of Yunnan Province from July 2023 to December 2023. MDSCs were isolated using MACS, followed by gene expression profiling with microarray analysis. Differential gene expression was analyzed using methods such as DESeq2 or edgeR to identify significant changes in gene expression between the two groups, with control of false positive rates through P-values and multiple corrections （e.g., FDR）. Enrichment analysis （e.g., GO or KEGG analysis） was performed to evaluate the enrichment of differential genes in biological pathways. The SPSS 16.0 software was utilized for hypergeometric tests or Fisher's exact tests to determine significance.   Results  Compared to normal pregnancies, 163 genes showed significant changes in the decidual MDSCs of URSA patients （P < 0.05）, with 67 genes upregulated and 96 genes downregulated. These genes were enriched in cellular components, biological processes, molecular functions, protein binding, complement system signaling pathways, leukocyte-related activation inflammatory response pathways, proteoglycans, and extracellular matrix receptor interactions. PPI network analysis and HUB gene identification showed that among the top 10 HUB genes, only SPP1, CCL5, C3AR1 and TNF were up-regulated, while MXRA8, IGFBP5, SPARCL1, SAA1, DCN and COL3A1 were down-regulated. The HUB gene were mainly involved in immune regulation, inflammatory responses and intercellular interactions.   Conclusion  MDSCs in the decidual of URSA patients exhibit impaired interactions with the extracellular matrix and other cells, resulting in decreased immunosuppressive capacity and enhanced pro-inflammatory responses.
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