miR-212-3p Targeted Regulation of NAP1L1 Inhibits Glioma Cell Proliferation，Migration and EMT

Xiaobing GUO; Xiaowen LI; Hengxi LI; Yan CAO; Ping LI

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Xiaobing GUO, Xiaowen LI, Hengxi LI, Yan CAO, Ping LI. miR-212-3p Targeted Regulation of NAP1L1 Inhibits Glioma Cell Proliferation，Migration and EMT[J]. Journal of Kunming Medical University.

Citation:

Xiaobing GUO, Xiaowen LI, Hengxi LI, Yan CAO, Ping LI. miR-212-3p Targeted Regulation of NAP1L1 Inhibits Glioma Cell Proliferation，Migration and EMT[J]. Journal of Kunming Medical University.

Xiaobing GUO, Xiaowen LI, Hengxi LI, Yan CAO, Ping LI. miR-212-3p Targeted Regulation of NAP1L1 Inhibits Glioma Cell Proliferation，Migration and EMT[J]. Journal of Kunming Medical University.

Citation:

Xiaobing GUO, Xiaowen LI, Hengxi LI, Yan CAO, Ping LI. miR-212-3p Targeted Regulation of NAP1L1 Inhibits Glioma Cell Proliferation，Migration and EMT[J]. Journal of Kunming Medical University.

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miR-212-3p Targeted Regulation of NAP1L1 Inhibits Glioma Cell Proliferation，Migration and EMT

Dept. of Human Anatomy and Histoembryology，School of Basic Medical Sciences，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2024-06-24
Available Online: 2024-11-09

Abstract

Abstract

Objective To explore the molecular mechanism of miR-212-3p in glioma cell proliferation, invasion and epithelial-mesenchymal transition （EMT）. Methods The expression of miR-212-3p and NAP1L1 were detected by RT-qPCR in glioma cells. NC mimic, miR-212-3p mimic, oe-NC and oe-NAP1L1 were built and transfected in cells. CCK-8, Transwell and wound healing assay were used to evaluate the cell biological behaviour. Western blot was used to detect the expression of EMT-related biomarkers. The relationship between miR-212-3p and NAP1L1 was confirmed by the dual-luciferase reporter gene and AgO2-RIP assay. Results miR-212-3p was lowly expressed in glioma cells （P < 0.0001）. miR-212-3p mimic significantly inhibited the glioma cell proliferation （P < 0.0001）, invasion （P = 0.0011） and migration （P < 0.0001）, and reduced the expression of EMT-related biomarkers N-cadherin （P = 0.000861） and Vimentin （P = 0.007430）, while upregulating the expression of E-cadherin （P < 0.0001）. miR-212-3p targeted and negatively regulated the NAP1L1 expression. Overexpression of NAP1L1 reversed the inhibitory effects of miR-212-3p on glioma cell proliferation （P < 0.0001）, migration （P < 0.0001）, and EMT （P < 0.0001）. Conclusion miR-212-3p inhibits the glioma cell proliferation, migration and EMT by targeting the negative regulation of NAP1L1 expression.
- Glioma,
- miR-212-3p,
- NAP1L1,
- Epithelial-mesenchymal transition,
- Cell proliferation

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References(32)

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