Effects of MOGAT1 and Androgen Receptor on the Pathogenesis of Acne in Mice

Hongchao ZOU; Wei ZHU; Ran SHEN; Rui LI; Zhao ZHANG

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Hongchao ZOU, Wei ZHU, Ran SHEN, Rui LI, Zhao ZHANG. Effects of MOGAT1 and Androgen Receptor on the Pathogenesis of Acne in Mice[J]. Journal of Kunming Medical University.

Citation:

Hongchao ZOU, Wei ZHU, Ran SHEN, Rui LI, Zhao ZHANG. Effects of MOGAT1 and Androgen Receptor on the Pathogenesis of Acne in Mice[J]. Journal of Kunming Medical University.

Hongchao ZOU, Wei ZHU, Ran SHEN, Rui LI, Zhao ZHANG. Effects of MOGAT1 and Androgen Receptor on the Pathogenesis of Acne in Mice[J]. Journal of Kunming Medical University.

Citation:

Hongchao ZOU, Wei ZHU, Ran SHEN, Rui LI, Zhao ZHANG. Effects of MOGAT1 and Androgen Receptor on the Pathogenesis of Acne in Mice[J]. Journal of Kunming Medical University.

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Effects of MOGAT1 and Androgen Receptor on the Pathogenesis of Acne in Mice

1.
Department of Dermatology
2.
Department of Obstetrics，Affiliated Yan’an Hospital of Kunming Medical University，Kunming Yunnan 650051
3.
Department of Dermatology，Kunming Children’s Hospital，Kunming Yunnan 650021，China

Received Date: 2025-12-10
Available Online: 2026-06-29

Abstract

Abstract

Objective To explore the mechanism by which monoacylglycerol O-acyltransferase 1 （MOGAT1） inhibits the occurrence and development of acne. Methods An acne mouse model was established by subcutaneous injection of Propionibacterium acnes （P. acnes）. An in vitro acne model was constructed by treating SZ95 cells with linoleic acid （LA）. RT-qPCR was used to detect mRNA levels of relevant factors, Western blot was used to detect protein expression, corresponding kits were used to detect reactive oxygen species （ROS） levels, catalase （CAT） content, and superoxide dismutase （SOD） activity. ELISA kits were used to detect interleukin-6 （IL-6）, interleukin-1 beta （IL-1β）, and tumor necrosis factor-alpha （TNF-α） levels in cells and tissues of each group. Oil Red O staining was used to detect lipid accumulation in cells, and hematoxylin and eosin （HE） staining was used to detect pathological changes in mouse skin tissue. Results Compared with the normal group, MOGAT1 mRNA and protein expression were significantly increased in skin tissue and cells of the model group mice （P < 0.001）. Knockdown of MOGAT1 alleviated the skin damage of the model mice, inhibited inflammatory factor expression, and the therapeutic effect of MOGAT1 knockdown was comparable to that of positive drug control. Knockdown of MOGAT1 enhanced the activity of SOD （P < 0.0001） and CAT （P < 0.0001）, reduced triglyceride content （P < 0.0001）, and in addition, MOGAT1 knockdown specifically inhibited the AR/SREBP-1 pathway and lipid metabolism. Conclusion MOGAT1 knockdown exerts a protective effect in the occurrence and development of acne by targeting and regulating the AR/SREBP-1 signaling axis, effectively inhibiting abnormal lipid accumulation and inflammatory responses.
- acne,
- MOGAT1,
- inflammation,
- lipid metabolism,
- androgen receptor

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References(17)

References

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