Mechanisms of Collagen-associated Integrin α1，α2 and β1 in the Caveolin-1/AQP1 Pathway During Scleral Remodeling in the Recovery Phase of Form-Deprivation Myopia

Lulu CHEN; Yabin DONG

Article Contents

Article Navigation > Journal of Kunming Medical University > 2026 > Uncorrected proof

Lulu CHEN, Yabin DONG. Mechanisms of Collagen-associated Integrin α1，α2 and β1 in the Caveolin-1/AQP1 Pathway During Scleral Remodeling in the Recovery Phase of Form-Deprivation Myopia[J]. Journal of Kunming Medical University.

Citation:

PDF( 1009 KB)

Mechanisms of Collagen-associated Integrin α1，α2 and β1 in the Caveolin-1/AQP1 Pathway During Scleral Remodeling in the Recovery Phase of Form-Deprivation Myopia

Lulu CHEN,
Yabin DONG^,

Department of Ophthalmology ，Xuzhou Central Hospital，Xuzhou Jiangsu 221000，China

Received Date: 2025-07-19
Available Online: 2026-01-19

Abstract

Abstract

Objective To investigate the mechanism of collagen-related integrins α1, α2 and β1 on the Caveolin-1 （Cav-1）/aquaporin 1 （AQP1） signaling pathway during scleral remodeling in the recovery phase of form-deprivation myopia （FDM）. Methods 50 guinea pigs were randomly divided into a study group （n = 40） and a control group （n = 10）. The study group underwent FDM induction with monocular occlusion for 4 consecutive weeks. After removing the eye cover, FDM guinea pig models at different recovery time points were established: 4-week deprivation, 3-day recovery, 7-day recovery, and 14-day recovery （n = 10 each）. The contralateral eye of study group animals served as internal control, and both eyes of the 10 control group animals served as normal control. Refraction and axial length of control eyes, 4-week deprivation eyes, 3-day recovery eyes, 7-day recovery eyes, and 14-day recovery eyes were measured. RT-PCR was used to detect the mRNA expression of collagen-related integrin α1, α2, and β1 in the scleral tissue; electron microscopy was used to observe the ultrastructure of the sclera; HE staining was used to observe the morphology of the sclera; and immunoblotting was used to detect the expression of Caveolin-1 and AQP1 proteins in the scleral tissue. Results Compared with the control eyes, 4-week deprivation eyes, 3-day recovery eyes, and 7-day recovery eyes showed decreased refraction and AQP1 protein, with increased axial length and Caveolin-1 （P < 0.0083）. At 14-day recovery, no significant changes were observed （P > 0.0083）. Compared with 4-week deprivation and 3-day recovery eyes, 7-day recovery eyes showed elevated refraction and AQP1 protein expression, with decreased axial length and Caveolin-1 （P < 0.0083）. No significant differences were found between 4-week deprivation and 3-day recovery eyes （P > 0.0083）. Compared with control eyes, Integrinα1, α2, and β1 mRNA levels were decreased in 4-week deprivation, 3-day recovery, and 7-day recovery eyes（P < 0.0083）, with no significant changes at 14-day recovery（P > 0.0083）. Compared with 4-week deprivation eyes, Integrinα1, α2 and β1 mRNA levels increased in 3-day and 7-day recovery eyes, with the highest levels at 7-day recovery （P < 0.0083）. Electron microscopy showed improved collagen fibril distribution in 7-day and 14-day recovery eyes, with 14-day recovery eyes showing near-normal distribution. HE staining revealed that 14-day recovery eyes had near-normal scleral morphology. Conclusion During scleral remodeling in the recovery period of form-deprivation myopia, collagen-related Integrins α1, α2 and β1 may directly or indirectly regulate the Caveolin-1/AQP1 pathway, affecting water transport and signal transduction in scleral cells, thereby participating in scleral remodeling.
- Form deprivation myopia,
- Scleral remodeling,
- Caveolin-1,
- Aquaporin-1,
- Integrin α1,
- Integrin α2,
- Integrin β1

FullText(HTML)

References(24)

References

[1]	李聪颖, 甘嘉禾, 王美君, 等. 不同强度光照对豚鼠屈光发育和形觉剥夺性近视的影响[J]. 中华实验眼科杂志, 2022, 40(6): 491-497. doi: 10.3760/cma.j.cn115989-20220228-00079
[2]	杨心怡, 黄洪鹏, 郭晓萱, 等. 新托品对豚鼠形觉剥夺性近视的抑制作用及机制[J]. 中国药理学与毒理学杂志, 2024, 38(5): 360-368. doi: 10.3867/j.issn.1000-3002.2024.05.005
[3]	Qian K W, Li Y Y, Wu X H, et al. Altered retinal dopamine levels in a melatonin-proficient mouse model of form-deprivation myopia[J]. Neurosci Bull, 2022, 38(9): 992-1006. doi: 10.1007/s12264-022-00842-9
[4]	Feng J, Zhang X, Li R, et al. Widespread involvement of acetylation in the retinal metabolism of form-deprivation myopia in guinea pigs[J]. ACS Omega, 2023, 8(26): 23825-23839. doi: 10.1021/acsomega.3c02219
[5]	Li J, Jo M H, Yan J, et al. Ligand binding initiates single-molecule integrin conformational activation[J]. Cell, 2024, 187(12): 2990-3005. e17.
[6]	Johnson R T, Solanki R, Wostear F, et al. Piezo1-mediated regulation of smooth muscle cell volume in response to enhanced extracellular matrix rigidity[J]. Br J Pharmacol, 2024, 181(11): 1576-1595. doi: 10.1111/bph.16294
[7]	张凯雪, 方文卿, 陈娟, 等. 约束视距下照度诱导近视动物模型的研究[J]. 眼科, 2023, 32(4): 294-298. doi: 10.13281/j.cnki.issn.1004-4469.2023.04.005
[8]	姚丽. 当归对形觉剥夺性气血不足型近视豚鼠脉络膜厚度的影响研究[D]. 沈阳: 辽宁中医药大学, 2023.
[9]	Cui Z, Huang Y, Chen X, et al. Identification of miR-671-5p and its related pathways as general mechanisms of both form-deprivation and lens-induced myopia in mice[J]. Curr Issues Mol Biol, 2023, 45(3): 2060-2072. doi: 10.3390/cimb45030132
[10]	Kanchanawong P, Calderwood D A. Organization, dynamics and mechanoregulation of integrin-mediated cell-ECM adhesions[J]. Nat Rev Mol Cell Biol, 2023, 24(2): 142-161.
[11]	Sawant M, Wang F, Koester J, et al. Ablation of integrin-mediated cell-collagen communication alleviates fibrosis[J]. Ann Rheum Dis, 2023, 82(11): 1474-1486. doi: 10.1136/ard-2023-224129
[12]	Metlapally R, Jobling A I, Gentle A, et al. Characterization of the integrin receptor subunit profile in the mammalian sclera[J]. Mol Vis, 2006, 12: 725-734.
[13]	李兴钰, 汤锦菲, 李菲菲. 藏红花酸调控EGR-1信号轴抑制近视模型小鼠巩膜重塑的作用机制研究[J]. 浙江中西医结合杂志, 2025, 35(5): 396-401+406. doi: 10.3969/j.issn.1005-4561.2025.05.002
[14]	张新, 巨朝娟, 金鑫, 等. 外源性神经生长因子对形觉剥夺性近视豚鼠巩膜组织的保护作用及其机制[J]. 吉林大学学报(医学版), 2021, 47(6): 1455-1461. doi: 10.13481/j.1671-587X.20210615
[15]	Shu Z, Chen K, Wang Q, et al. The role of retinal dopamine D1 receptors in ocular growth and myopia development in mice[J]. J Neurosci, 2023, 43(48): 8231-8242. doi: 10.1523/JNEUROSCI.1196-23.2023
[16]	Dong J, Hu L, Li C, et al. Expression of osteopontin and integrin αvβ3 receptor in retina of diabetic guinea pigs with high myopia[J]. Ophthalmic Res, 2023, 66(1): 144-150. doi: 10.1159/000526586
[17]	王园. 胶原相关整合素α1、α2和β1在形觉剥夺性近视诱导及恢复期中调节巩膜重塑作用的研究[D]. 青岛: 青岛大学, 2015.
[18]	Liu X, Yuan Y, Wu Y, et al. Extracellular matrix stiffness modulates myopia scleral remodeling through integrin/F-actin/YAP axis[J]. Invest Ophthalmol Vis Sci, 2025, 66(2): 22. doi: 10.1167/iovs.66.2.22
[19]	张悦之, 邓燕, 殷小龙, 等. 人巩膜成纤维细胞中miR-184表达与近视发生、发展的关系及其对PI3K-Akt信号通路的调控作用[J]. 眼科新进展, 2023, 43(6): 444-448. doi: 10.13389/j.cnki.rao.2023.0089
[20]	Stone R A, Tobias J W, Wei W, et al. Diurnal gene expression patterns in retina and choroid distinguish myopia progression from myopia onset[J]. PLoS One, 2024, 19(7): e0307091. doi: 10.1371/journal.pone.0307091
[21]	Chen W, Li Z, Wang Q, et al. The role of C-Jun N-terminal kinase-1 in controlling aquaporin-1 and choroidal thickness during recovery from form-deprivation myopia in guinea pigs[J]. Curr Eye Res, 2021, 46(6): 885-894. doi: 10.1080/02713683.2020.1839107
[22]	Li Z, Chen W, Zhang H, et al. The aquaporin-1 depletion downregulates the sclera biomechanical strength[J]. Curr Eye Res, 2020, 45(10): 1240-1244. doi: 10.1080/02713683.2020.1730404
[23]	王绍娟, 杨菁. 整合素调节Fak/SFKs与生长因子受体信号传导的研究进展[J]. 国际免疫学杂志, 2014, 37(4): 307-311. doi: 10.3760/cma.j.issn.1673-4394.2014.04.011
[24]	Petpiroon N, Bhummaphan N, Tungsukruthai S, et al. Chrysotobibenzyl inhibition of lung cancer cell migration through Caveolin-1-dependent mediation of the integrin switch and the sensitization of lung cancer cells to cisplatin-mediated apoptosis[J]. Phytomedicine, 2019, 58: 152888. doi: 10.1016/j.phymed.2019.152888

Relative Articles(20)

Relative Articles

[1]	Jinyan ZHOU, Kerong LI, Yan MA, Jiqiang WANG, Zhengming ZHANG, Wang LI. Investigation and Analysis of Vitamin K Level Distribution in 1177 Infants of Different Age Groups. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20240114
[2]	Liyuan WANG, Zhenting YANG, Qingling LI, Haiwen LI, Zhirong ZHAO, Yongrui YANG, Shude LI. Effects of Scutellarin on MMP2，MMP9 and TIMP-1 Protein in Liver of Obese Rats with Chronic Liver Injury by High Glucose and Fat Diet. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20230408
[3]	Yixiao LIU, Mengyuan SHOU, Ying ZHANG, Rongqiang ZHANG, Na SUN. Expression of CAV1 in Tumor Tissues and Its Effect on Survival Prognosis of Patients. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20231107
[4]	Zhenkun LI, Tuanli BAI, Linyu YANG, Ying Wang. Breviscapine Regulates the mRNA and Protein Levels of CINC-1 and CINC-2 in the Right Ventricle of Rats with Acute Pulmonary Embolism. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20221002
[5]	Wang Bo , Xia Xian Song , Li Jin Tao , Duan Xiao Hua . . Journal of Kunming Medical University,
[6]	Zhang Xiao Chao , Yang Ren Hua , Shen Ying , He Hong Yan , Zhong Jin Ping , Shen Zhi Qiang , Chen Peng . Effects of Geraniin on Modulating the Expression of Cbfα 1 Gene in Rabbit Osteoporotic Fracture. Journal of Kunming Medical University,
[7]	He Feng Die . The Changes of Serum CRP， IL-2， MCP-1 and Hemorheology of Patients with Pelvic Inflammatory Disease. Journal of Kunming Medical University,
[8]	Lin Na . . Journal of Kunming Medical University,
[9]	Zhou Kai Hua . . Journal of Kunming Medical University,
[10]	Zhang Fan . . Journal of Kunming Medical University,
[11]	Zhang Hong . . Journal of Kunming Medical University,
[12]	Yang Qiu Ping . . Journal of Kunming Medical University,
[13]	Tao Jian Ping . . Journal of Kunming Medical University,
[14]	Wu Guo Jiu . . Journal of Kunming Medical University,
[15]	Lin Yan . . Journal of Kunming Medical University,
[16]	Gu Wan Gang . . Journal of Kunming Medical University,
[17]	Shao Jian Lin . . Journal of Kunming Medical University,
[18]	Yang Li Hua . . Journal of Kunming Medical University,
[19]	. Relationship between Expression of CDK2 and p27kip1 Protein and Non-small Cell Lung Cancer in Xuanwei. Journal of Kunming Medical University,
[20]	Li Xiao Ming . . Journal of Kunming Medical University,