Effect of Rapamycin-induced Oxidative Stress on Thyroiditis Rats

Nan HUAI; Rui LI; Guangrong SONG; Anren KUANG

Article Contents

Article Navigation > Journal of Kunming Medical University > 2025 > Accepted Manuscript

Nan HUAI, Rui LI, Guangrong SONG, Anren KUANG. Effect of Rapamycin-induced Oxidative Stress on Thyroiditis Rats[J]. Journal of Kunming Medical University.

Citation:

Nan HUAI, Rui LI, Guangrong SONG, Anren KUANG. Effect of Rapamycin-induced Oxidative Stress on Thyroiditis Rats[J]. Journal of Kunming Medical University.

Nan HUAI, Rui LI, Guangrong SONG, Anren KUANG. Effect of Rapamycin-induced Oxidative Stress on Thyroiditis Rats[J]. Journal of Kunming Medical University.

Citation:

Nan HUAI, Rui LI, Guangrong SONG, Anren KUANG. Effect of Rapamycin-induced Oxidative Stress on Thyroiditis Rats[J]. Journal of Kunming Medical University.

PDF( 1263 KB)

Effect of Rapamycin-induced Oxidative Stress on Thyroiditis Rats

1.
Department of Endocrinology，Hengshui People’s Hospital
2.
Gynecology Department of Hengshui People’s Hospital，Hengshui Hebei 053000，China

Received Date: 2024-06-16

Abstract

Abstract

Objective To investigate the effect of rapamycin-induced oxidative stress on thyroiditis rats. Methods Forty rats were randomly divided into 4 groups : normal control group, model group, dexamethasone group and rapamycin group, with 10 rats in each group. Except for the normal control group, a rat model of thyroiditis was constructed and rapamycin was intervened. The levels of reactive oxygen species （ROS） and glutathione （GSH） in thyroid tissue of rats were detected by colorimetry. HE staining was used to observe the pathological morphology ; the expression of chemokine receptor （CXCR3） and C-C chemokine receptor （CCR4） in thyroid tissue of rats was detected by PCR. The phosphorylation level of rapamycin target protein （mTOR） and the level of autophagy protein microtubule-associated protein 1 light chain 3α-II （LC3-II） protein in thyroid tissue of rats were detected by immunohistochemistry. Western blot was used to detect the expression of CXCR3, CCR4, Beclin-1 and LC3 protein in thyroid tissue of rats. Results Compared with the normal control group, the levels of ROS, CXCR3, CXCR3 mRNA expression and the number of p-mTOR positive cells in the thyroid tissue of the model group were increased （P < 0.05）, while the levels of GSH, CCR4, Beclin-1, LC3, CCR4 mRNA expression and the number of LC3-II positive cells were decreased （P < 0.05）. Compared with the model group, the levels of ROS, CXCR3, CXCR3 mRNA expression and the number of p-mTOR positive cells in the dexamethasone group and the rapamycin group were decreased （P < 0.05）, and the rapamycin group was lower（P < 0.05）. The levels of GSH, CCR4, Beclin-1 and LC3, the expression of CCR4 mRNA and the number of LC3-II positive cells increased （P < 0.05）, and the rapamycin group was higher （P < 0.05）. Conclusion Rapamycin can improve the level of oxidative stress, inhibit the expression of p-mTOR, increase the level of LC3-II and autophagy in rats with thyroiditis, and regulate the expression of CXCR3/CCR4 protein.
- Rapamycin,
- Oxidative Stress,
- Thyroiditis,
- Autophagy,
- C-C Chemokine Receptor Type 4

FullText(HTML)

References(19)

References

[1]	Albano D, Dondi F, Zilioli V, et al. The role of Hashimoto thyroiditis in predicting radioiodine ablation efficacy and prognosis of low to intermediate risk differentiated thyroid cancer[J]. Ann Nucl Med, 2021, 35(10): 1089-1099. doi: 10.1007/s12149-021-01644-1
[2]	李方远, 陆华, 王通, 等. 接绪资冲颗粒对实验性自身免疫性甲状腺炎致卵巢储备功能下降小鼠肠道菌群的影响[J]. 中华中医药杂志, 2023, 38(8): 3799-3804.
[3]	Poillet-Perez L, Sarry J E, Joffre C. Autophagy is a major metabolic regulator involved in cancer therapy resistance[J]. Cell Reports, 2021, 36(7): 109528. doi: 10.1016/j.celrep.2021.109528
[4]	张雪琪, 张帆, 滕卫平, 辅助性T细胞17介导桥本甲状腺炎发病机制的研究现状. 中华内分泌代谢杂志, 2022, 38(11): 1001-1005.
[5]	Yu X, J Wu, Wu Q, et al. Quantitative analysis of autophagy-related protein LC3B by quantum-dot- based molecular imaging Chapter outline[J]. Methods in Cell Biology, 2021, 165(4): 177-185.
[6]	林泽杭, 段志敏, 徐松, 等. 白念珠菌菌丝调控小鼠巨噬细胞自噬关键分子微管相关蛋白1轻链3的实验研究[J]. 中华皮肤科杂志, 2021, 54(3): 189-195.
[7]	赵连春, 牛丽霞, 赵振军, 等. CXC趋化因子受体3对甲状腺细胞自噬与炎症的影响及机制[J]. 安徽医学, 2024, 45(5): 542-548.
[8]	Lu Q, Dou Y, Yu T, et al. Association of thyroglobulin antibody and thyroid peroxidase antibody status with aggressive features in papillary thyroid carcinoma with Hashimoto's thyroiditis[J]. Gland Surg, 2025, 14(6): 1091-1100. doi: 10.21037/gs-2025-47
[9]	Zhang L, Sun X, Liu L, et al. Excessive iodine induces thyroid follicular epithelial cells apoptosis by activating HIF-1α-mediated hypoxia pathway in Hashimoto thyroiditis[J]. Mol Biol Rep., 2023, 50(4): 3633-3640. doi: 10.1007/s11033-023-08273-z
[10]	李会云, 孟冉, 迟茹雪, 等. 雷帕霉素上调线粒体自噬保护脓毒症大鼠下丘脑功能[J]. 现代生物医学进展, 2025, 25(5): 812-821, 854.
[11]	贾思锋, 张卓, 段昱宇, 等. 基于中西医临床病证特点的自身免疫性甲状腺炎动物模型分析[J/OL]. 中国实验方剂学杂志, 1-11 5-08-09
[12]	吴菁华. 不同治疗方案对自身免疫性甲状腺炎的疗效比较[D]. 广州: 广州医科大学, 2023.
[13]	万斌, 陈正涛, 冷玉琳, 等. 肠道菌群和桥本甲状腺炎的关系及中医药调控研究进展[J]. 世界科学技术-中医药现代化, 2025, 27(3): 626-632.
[14]	巩博深, 单忠艳. 肠道菌群与炎症小体在自身免疫性甲状腺炎中的相互调控[J]. 国际内分泌代谢杂志, 2021, 41(4): 309-312.
[15]	da Silva G B, Yamauchi M A, Bagatini M D. Oxidative stress in Hashimoto's thyroiditis: possible adjuvant therapies to attenuate deleterious effects[J]. Mol Cell Biochem., 2023, 478(4): 949-966. doi: 10.1007/s11010-022-04564-4
[16]	蒋学林, 刘长江. 基于Akt/mTOR信号通路探讨洋地黄毒苷调控甲状腺滤泡上皮细胞自噬与凋亡的机制[J]. 现代中西医结合杂志, 2024, 33(4): 457-464.
[17]	何呈燕, 李依雯, 甘玲, 等. PI3K/AKT/mTOR信号通路通过调控Th17细胞分化参与EAT小鼠甲状腺自身免疫损伤[J]. 中国医科大学学报, 2024, 53(11): 972-978.
[18]	Dağdeviren M, Or Koca A, Akkan T, et al. Is oxidative stress a factor in the pathogenesis of subacute thyroiditis?[J]. Endokrynol Pol., 2022, 73(1): 64-70.
[19]	刘梅, 黄晶, 王军, 等. 碘化钾通过诱导PARP1表达活化NF-κB/NLRP3炎症小体促进甲状腺滤泡细胞焦亡[J]. 中华内分泌代谢杂志, 2021, 37(9): 820-829.

Relative Articles(20)

Relative Articles

[1]	Shuangxiu LI, Qi ZHENG, Gaosheng YIN, Ping YANG, Lu LING. Effect of Autophagic Flux Impairment-mediated Apoptosis on Pressure Overload-induced Heart Failure. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20250906
[2]	Xiaoxiao FAN, Xuan ZENG, Pingping ZHOU, Xi LIU, Feifei ZHU, Yanji LUO, Yi WU. Relationship between the Platelet Autophagy-related Factor Expression and Peritoneal Metastasis of Gastric Cancer. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20250715
[3]	Ximeng SONG, Shilong YUAN, Zi'ang LU, Maotong XU. Exploring the Molecular Mechanism of Ligustrazine in Spinal Cord Injury Repair Based on Macrophage Autophagy. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20250705
[4]	Kongjia WU, Shuqian DOU, Yuan WANG, Qi WANG, Pan DU, Wenjun LIU. Impact of PINK1/Parkin-Mediated Mitophagy in the Development of Neurodegenerative Diseases. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20250601
[5]	Egao YIN, Wen LEI, Mei YANG, Yongjun LIU, Zhaoxing DONG. Effect of mTOR Signaling Pathway Inhibition on Bleomycin-induced Pulmonary Fibrosis. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20250608
[6]	Yilin ZHU, Xiao PENG, Guifu ZHANG, Huinan LONG. Effects of Autophagy on Chondrocyte Apoptosis in Osteoarthritis: An Investigation Based on lncRNA/Hedgehog Signaling Pathway Expression. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20250605
[7]	Yanping LI, Efty Fariha Tasnim, Zhixing LU, Lingying ZHU. The Role of APOE4 in Regulating LRP1 on Aβ_25-35-Induced Oxidative Stress and Inflammatory Response in Astrocytes. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20251103
[8]	Ying TAN, Haiyan QIN, Xiang SUN, Yanyi SU, Yingbao WANG. Propofol Regulates MPP+-induced Mitochondrial Oxidative Stress and Apoptosis in SH-SY5Y Cells. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20240305
[9]	Chenchen LIANG, Jianpeng GAO. MSCs Promote Autophagy to Alleviate Liver Injury in NASH by Regulating AMPK/mTOR Axis. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20230728
[10]	Shi CHEN, Shi FU, Zhiyong TAN, Jiansong WANG, Haifeng WANG. Research Progress of Autophagy in the Development and Treatment of Bladder Cancer. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20230503
[11]	Li DONG, Shibo SUN, Shuguang SUN. Advances on Scutellarin Anti-Oxidative Stress Mechanism in the Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20220423
[12]	Yong-jun LIU, Yin WANG, Mei YANG, E-gao YIN, Ting LI, Zhao-xing DONG. The Effect of Hydrogen-Regulated Autophagy on Pulmonary Fibrosis in Paraquat Poisoned Rats. Journal of Kunming Medical University, doi: 10.12259/j.issn.2095-610X.S20210918
[13]	Zeng Qing Ju , Li Hui , Wang Jin Wen . . Journal of Kunming Medical University,
[14]	Guo An Li , Fu Chang Bi , Yang Xiao Qing , Zhang Shi Mei , Shi Zheng Juan . . Journal of Kunming Medical University,
[15]	Zeng Bai Rui . . Journal of Kunming Medical University,
[16]	Liu Song . . Journal of Kunming Medical University,
[17]	Ding Yan Jie . . Journal of Kunming Medical University,
[18]	Zhang Yuan . . Journal of Kunming Medical University,
[19]	Yan Qing Feng . Effects of Luteolin on Isolated Heart Function and Oxidative Stress Reaction after Hypothermic Preservation. Journal of Kunming Medical University,
[20]	Gui Li . . Journal of Kunming Medical University,