Molecular Epidemiological Investigation of the Fourth Human Case of Eurasian Avian-like H1N1 Swine Influenza Virus Infection in Yunnan Province

Yanhong SUN; Yaoyao CHEN; Jienan ZHOU; Meiling ZHANG; Zhaosheng LIU; Xiaonan ZHAO

doi:10.12259/j.issn.2095-610X.S20250404

Volume 46 Issue 4

Apr. 2025

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Yanhong SUN, Yaoyao CHEN, Jienan ZHOU, Meiling ZHANG, Zhaosheng LIU, Xiaonan ZHAO. Molecular Epidemiological Investigation of the Fourth Human Case of Eurasian Avian-like H1N1 Swine Influenza Virus Infection in Yunnan Province[J]. Journal of Kunming Medical University, 2025, 46(4): 20-28. doi: 10.12259/j.issn.2095-610X.S20250404

Citation:

Yanhong SUN, Yaoyao CHEN, Jienan ZHOU, Meiling ZHANG, Zhaosheng LIU, Xiaonan ZHAO. Molecular Epidemiological Investigation of the Fourth Human Case of Eurasian Avian-like H1N1 Swine Influenza Virus Infection in Yunnan Province[J]. Journal of Kunming Medical University, 2025, 46(4): 20-28. doi: 10.12259/j.issn.2095-610X.S20250404

Citation:

Yanhong SUN, Yaoyao CHEN, Jienan ZHOU, Meiling ZHANG, Zhaosheng LIU, Xiaonan ZHAO. Molecular Epidemiological Investigation of the Fourth Human Case of Eurasian Avian-like H1N1 Swine Influenza Virus Infection in Yunnan Province[J]. Journal of Kunming Medical University, 2025, 46(4): 20-28. doi: 10.12259/j.issn.2095-610X.S20250404

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Molecular Epidemiological Investigation of the Fourth Human Case of Eurasian Avian-like H1N1 Swine Influenza Virus Infection in Yunnan Province

doi: 10.12259/j.issn.2095-610X.S20250404

Yunnan Center for Disease Control and Prevention，Kunming Yunnan 650506，China

Received Date: 2024-12-12
Available Online: 2025-04-05
Publish Date: 2025-04-25

Abstract

Abstract

Objective To conduct a unique and pioneering molecular epidemiological investigation of a case of Eurasian avian-like H1N1 swine influenza identified in Yunnan Province in 2022 （the fourth such case in the province） and to understand its genetic characteristics so as to reveal its potential impact on human health. Methods Real-time fluorescent quantitative PCR detection technology was used for the nucleic acid testing of the case's pharyngeal swab samples, close contacts, and environmental samples from the living area. Positive samples were subjected to virus isolation using MDCK cells. Cell cultures were authenticated using erythrocyte agglutination assay with guinea pig blood and real-time fluorescence quantitative RT-PCR. Whole genome sequencing was performed using the Illumina MiseqNext-generation sequencing platform, and a phylogenetic tree was constructed using MEGA 7.0 software to analyze the genetic molecular characteristics. Results The first G5 genotype Eurasian avian-like H1N1 swine influenza virus in Yunnan Province was successfully isolated, and the whole genome sequence of the virus was obtained. This virus possessed the molecular characteristics associated with increased adaptability, virulence, or transmissibility in mammals and had a nucleotide consistency of 99.2%～99.7% with a porcine strain isolated in Jiangsu province. These findings underscored the potential threat this virus poses to human health. Conclusion The study underscores the importance of further monitoring swine influenza in preventing new influenza virus subtypes that can infect humans.
- Eurasian avian-like H1N1 swine influenza virus,
- Next-generation sequencing,
- Phylogenetic analysis,
- Amino acid characterization

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References(28)

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