Expression of Zinc Finger Transcription Factor Sall4 In Pancreatic Cancer and Its Impact on Cell Invasion and Migration

Qingbin ZENG; Rong Xu; Wenzhi DONG; Zhijian CHEN; Weiran LIAO; Kui LONG

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Qingbin ZENG, Rong Xu, Wenzhi DONG, Zhijian CHEN, Weiran LIAO, Kui LONG. Expression of Zinc Finger Transcription Factor Sall4 In Pancreatic Cancer and Its Impact on Cell Invasion and Migration[J]. Journal of Kunming Medical University.

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Expression of Zinc Finger Transcription Factor Sall4 In Pancreatic Cancer and Its Impact on Cell Invasion and Migration

1.
Dept. of Hepatobiliary and Pancreatic Surgery，The 2 Affiliated Hospital of Yunnan Kunming Medical University，Kunming Yunnan 65000
2.
Department of Anesthesiology and Surgery，The 2nd Affiliated Hospital of Yunnan University，Kunming Yunnan 65000，China

Received Date: 2024-02-03

Abstract

Abstract

Objective To investigate the expression of spalt like transcription factor 4 （Sall4） in pancreatic cancer tissues and its clinical significance, as well as the impact of inhibiting its gene expression on the migration and invasion of pancreatic cancer cells. Methods This study involved 64 patients with pancreatic cancer treated at the Second Affiliated Hospital of Kunming Medical University from January 2014 to January 2018. Immunohistochemical staining was used to detect the expression of Sall4 protein in adjacent non-tumor tissues and pancreatic cancer tissues, respectively. Real-time quantitative PCR was used to measure the mRNA expres levels of Sall4 in the cancerous and adjacent non-tumor tissues, respectively.The relationship between the clinicopathological data of these patients and the expression of Sall4 protein was also analyzed. The cells of high Sall4 expression were screened from the human pancreatic cancer cell lines PANC-1, Capan-1, SW 1990, HPAC, and HPAF-II. The pancreatic cancer cells of high Sall4 expression were divided into three groups: control group, shRNA-1 group, and shRNA-2 group. The shRNA-1 and shRNA-2 groups were transfected with the corresponding Sall4 lentiviral inhibitory gene sequences, while the control group was transfected with the reagent without an interference sequence. Western blotting was used to measure the expression of Sall4, and cells with significantly reduced expression were selected for subsequent experiments. The transfected cells were then assessed for their invasive and migratory abilities. Results Among the 64 samples of pancreatic cancer tissues, 28 cases （43.8%） were positive for Sall4 expression, a rate significantly higher than 5 cases （7.8%） in adjacent non-tumor tissues. Furthermore, 16 cases exhibited strong positivity, while 12 cases showed moderate or weak positivity. Recurrence occurred in 11 pancreatic cancer patients. The difference was statistically significant. The expression of Sall4 was correlated with tumor differentiation, staging, and lymph node metastasis, suggesting that Sall4 positivity might be an independent risk factor affecting the prognosis of pancreatic cancer. Among the five cell lines, PANC-1 had the highest relative expression of Sall4 and was selected for further experiments. The shRNA-1 and shRNA-2 groups successfully suppressed the expression of Sall4, with the shRNA-1 group showing a more pronounced effect. Compared to the control group, the invasive and migratory abilities of SW480 cells were significantly reduced in the shRNA-1 group （P < 0.05）. Conclusion Sall4 is highly expressed in pancreatic cancer tissues, and higher expression is associated with a greater likelihood of postoperative recurrence. Inhibiting the expression of Sall4 can significantly suppress the invasion and migration abilities of human pancreatic cancer cells.
- Pancreatic cancer,
- Zinc finger transcription factor 4,
- Cell invasion,
- Cell migration

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References(17)

References

[1]	中华人民共和国国家卫生健康委员会医政医管局. 胰腺癌诊疗指南2022年版)[J]. 中华消化外科杂志,2022,21(9):1117-1136. doi: 10.3760/cma.j.cn115610-20220726-00431
[2]	王军华,王锐炫,胰腺癌新辅助化疗现状和治疗策略. 中华肝脏外科手术学电子杂志,2024,13(5) : 640-643.
[3]	Fatma A. Abouelnazar,Xiaoxin Zhang,Maoye Wang,et al. The new advance of SALL4 in cancer: Function,regulation,and implication. Journal of Clinical Laboratory Analysis. 2023;37 (9): 1-16.
[4]	Zhang Y,Bharadwaj U,Logsdon C D ,et al. ZIP4 regulates pancreatic cancer cell growth by activating IL-6/STAT3 pathway through zinc finger transcription factor CREB. [J]. Clinical Cancer Research,2010,16(5): 1423-1430.
[5]	Maity G,Haque I,Ghosh A,et al. The MAZ transcription factor is a downstream target of the oncoprotein Cyr61/CCN1 and promotes pancreatic cancer cell invasion via CRAF-ERK signaling[J]. Journal of Biological Chemistry,2018,293(12):4334-4349.
[6]	Wei C Y,Zhu M X,Zhang P F,et al. PKCα/ZFP64/CSF1 axis resets the tumor microenvironment and fuels anti-PD1 resistance in hepatocellular carcinoma. J Hepatol. 2022,77(1): 163-176.
[7]	Huang W,Li N,Hu J,et al. Inhibitory effect of RNA-mediated knockdown of zinc finger protein 91 pseudogene on pancreatic cancer cell growth and invasion[J]. Oncology Letters,2016,12(5):1343-1348.
[8]	Shidan Liu,Jiaxi Luan,Yan Ding,et al. miR-144-3p Targets FosB Proto-oncogene,AP-1Transcription Factor Subunit (FOSB) to Suppress Proliferation,Migration,and Invasion of PANC-1 Pancreatic Cancer Cells[J]. Oncology Research,2018,26(5):683-690. doi: 10.3727/096504017X14982585511252
[9]	Inaguma S,Ito H,Riku M ,et al. Addiction of pancreatic cancer cells to zinc-finger transcription factor ZIC2[J]. Oncotargt,2015,6(29): 28257-28268;
[10]	Vienot A,Monnien F,Truntzer C,et al. SALL4-related gene signature defines a specific stromal subset of pancreatic ductal adenocarcinoma with poor prognostic features. Mol Oncol. 2023,17(7): 1356- 1378.
[11]	Jung J,Park S,Jang Y,et al. Clinical significance of glycolytic metabolic activity in hepatocellular carcinoma. Cancers (Basel). 2022,15(186): 1-14.
[12]	Ding Y,Cho M,Chan A,et al. SALL4 is expressed in pancreatic acinar cell carcinoma[J]. Case Reports in Clinical Pathology,2014,1(2):36-41;
[13]	Hotz B,Visekruna A,Buhr H J,et al. Beyond epithelial to mesenchymal transition: a novel role for the transcription factor snail in inflammation and wound healing[J]. Journal of Gastrointestinal Surgery,2010,14(2):388-397. doi: 10.1007/s11605-009-1068-3
[14]	Dengfeng C,Humphrey P A,Allan R W. SALL4 is a novel sensitive and specific marker for metastatic germ cell tumors,with particular utility in detection of metastatic yolk sac tumors[J]. Cancer,2019,(115)12: 2640-51.
[15]	Akimasa Seno,Tomonari Kasai,Masashi Ikeda,et al. Characterization of Gene Expression Patterns among Artificially Developed Cancer Stem Cells Using Spherical Self-Organizing Map[J]. Cancer Informatics,2016,15(5):163-178;
[16]	Sherma Zibadi et al. "SALL4 expression in gastrointestinal and pancreatic adenocarcinoma and hepatocellular carcinoma: an immunohistochemical study". American Journal of Clinical Pathology ,2014(1): 214-218;
[17]	Xingding Zhang,Jing Yu,Yang Qin,et al. The zinc finger transcription factor ZKSCAN3 promotes prostate cancer cell migration. The International Journal of Biochemistry & Cell Biology. 2012;44 (7): 1166-1173.

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