Characteristics of katG and inhA Gene Mutations in INH-Resistant Mycobacterium Tuberculosis in Yunnan Province

Lianyong CHEN; Haohao RU; Xing YANG; Shuangqun YAN; Tao CHEN; Qinxuan NI; Lin XU

doi:10.12259/j.issn.2095-610X.S20220803

Volume 43 Issue 8

Jul. 2022

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Article Navigation > Journal of Kunming Medical University > 2022 > 43(8): 28-33

Lianyong CHEN, Haohao RU, Xing YANG, Shuangqun YAN, Tao CHEN, Qinxuan NI, Lin XU. Characteristics of katG and inhA Gene Mutations in INH-Resistant Mycobacterium Tuberculosis in Yunnan Province[J]. Journal of Kunming Medical University, 2022, 43(8): 28-33. doi: 10.12259/j.issn.2095-610X.S20220803

Citation:

Lianyong CHEN, Haohao RU, Xing YANG, Shuangqun YAN, Tao CHEN, Qinxuan NI, Lin XU. Characteristics of katG and inhA Gene Mutations in INH-Resistant Mycobacterium Tuberculosis in Yunnan Province[J]. Journal of Kunming Medical University, 2022, 43(8): 28-33. doi: 10.12259/j.issn.2095-610X.S20220803

Citation:

Lianyong CHEN, Haohao RU, Xing YANG, Shuangqun YAN, Tao CHEN, Qinxuan NI, Lin XU. Characteristics of katG and inhA Gene Mutations in INH-Resistant Mycobacterium Tuberculosis in Yunnan Province[J]. Journal of Kunming Medical University, 2022, 43(8): 28-33. doi: 10.12259/j.issn.2095-610X.S20220803

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Characteristics of katG and inhA Gene Mutations in INH-Resistant Mycobacterium Tuberculosis in Yunnan Province

doi: 10.12259/j.issn.2095-610X.S20220803

Dispensary for Tuberculosis Control and Prevention，Yunnan Center for Disease Control and Prevention，Kunming Yunnan 650022，China

Received Date: 2022-04-15
Available Online: 2022-07-19
Publish Date: 2022-07-28

Abstract

Abstract

Objective To analyze the characteristics of katG and inhA genetic mutations associated with INH-resistant Mycobacterium tuberculosis in Yunnan Province. Methods The INH-resistant Mycobacterium tuberculosis isolates were collected from drug-resistant monitoring sites in Yunnan Province, and the katG and inhA genes were amplified by PCR and then sequenced. Results Among the 88 MTB strains, the coincidence rate of INH-resistant genotype and phenotype was 82.95% （73/88）. 75.00% （66/88） had katG mutations and 9.09% （8/88） had inhA mutations. katG315 （67.05%） and inhA-15 （7.95%） were the top two mutation sites, and a high prevalence of katG Ser315Thr mutations （59.09%, 52/88） was observed. The frequency of the katG mutation was significantly higher in MDR-TB compared to INH mono-resistant isolates （χ² = 4.190, P = 0.041）, while katG315, inhA and inhA-15T mutations did not differ significantly between these two groups （P > 0.05）. Conclusion The most common mutation of INH-resistant Mycobacterium tuberculosis in Yunnan Province were katG315 and inhA-15, and katG mutation was higher among MDR isolates compared with INH mono-resistant strains. Molecular diagnostics based on gene mutations was a useful method for rapid detection of INH-resistant Mycobacterium tuberculosis in Yunnan Province.
- Mycobacterium tuberculosis,
- INH,
- Drug resistance,
- katG,
- inhA

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