Efficacy of T-SPOT.TB in Treatment of Active Tuberculosis

Rong WAN; Ming-wu LI; Meng MA; Ming-hong LAI; Guang-mei Li

doi:10.12259/j.issn.2095-610X.S20201237

Volume 41 Issue 12

Dec. 2019

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2020 > 41(12): 133-137

Rong WAN, Ming-wu LI, Meng MA, Ming-hong LAI, Guang-mei Li. Efficacy of T-SPOT.TB in Treatment of Active Tuberculosis[J]. Journal of Kunming Medical University, 2020, 41(12): 133-137. doi: 10.12259/j.issn.2095-610X.S20201237

Citation:

Rong WAN, Ming-wu LI, Meng MA, Ming-hong LAI, Guang-mei Li. Efficacy of T-SPOT.TB in Treatment of Active Tuberculosis[J]. Journal of Kunming Medical University, 2020, 41(12): 133-137. doi: 10.12259/j.issn.2095-610X.S20201237

Citation:

PDF( 555 KB)

Efficacy of T-SPOT.TB in Treatment of Active Tuberculosis

doi: 10.12259/j.issn.2095-610X.S20201237

No.2 Dept. of Tuberculosis，The 3rd People’s Hospital of Kunming，Kunming Yunnan 650041，China

Received Date: 2020-04-23
Publish Date: 2020-12-25

Abstract

Abstract

Objective To explore the significance of T-SPOT.TB test for monitoring the therapeutic effect of tuberculosis. Methods Using T-SPOT.TB kit, changes in response to ESAT-6 and CFP-10 were compared in 79 patients with active pulmonary tuberculosis before and after 2 months of treatment. Results In 79 patients with active pulmonary tuberculosis, The spot number of ESAT-6 and CFP-10 responses decreased significantly at the end of 2 months and at the end of treatment compared with the beginning of treatment, and the difference was statistically significant（P < 0.05）. The negative rates of ESAT-6 and CFP-10 at the end of 2 months and at the end of treatment were higher than those at the beginning of treatment, but there was no significant difference between them（ P > 0.05）. The T-SPOT.TB negative conversion rate of all patients was 3.8%.There was a statistical difference in ESAT-6 response between the end of 2 months and the end of treatment（ χ² = 4.635, P = 0.031 < 0.05）.There was no significant difference in CFP-10 response at the end of treatment 2 and at the end of treatment（ χ² = 1.272, P = 0.259 > 0.05）. Conclusion There were significant differences in ESAT-6 and CFP-10 of T-SPOT.TB responses before and after treatment. T-SPOT has a certain significance in evaluating of the therapeutic effect of tuberculosis, but its negative conversion ration can not be used as the indicator of ending the TB treatment.
- T-SPOT.TB,
- ESAT - 6,
- CFP - 10,
- Tuberculosis,
- Treatment effect

FullText(HTML)

References(14)

References

[1]	WHO. Global tuberculosis report 2017[EB/OL]. [2018-02-08]. http://www.who.int/tb/publications/globalreport/en/.
[2]	Lalvani A, Pathan A A, Mcshane H, et al. Rapid detection of Mycobacterium tuberculosis infection by enumeration of antigen-specific T cells[J]. Am J Respir Crit Care Med. 2001, 163（4）: 824-828. doi: 10.1164/ajrccm.163.4.2009100
[3]	中华人民共和国国家卫生和计划生育委员会. W288-2017, 中华人民共和国卫生行业标准-肺结核诊断[S]北京: 人民卫生出版社, 2017: 2-3.
[4]	T Meier, H-P Eulenbruch, P Wrighton-Smith, et, al. Sensitivity of a new commercial enzyme-linked immunospot assay（T.SPOT-TB）for diagnosis of tuberculosis in clinical practice[J]. Eur J Clin Microbiol Infect Dis. 2005, 24（8）: 529-536. doi: 10.1007/s10096-005-1377-8
[5]	Lalvani A, Pareek M. Interferon galnill8 release assays: principles and practice[J]. Enferm lnfece Micmbiol Clin. 2010, 28（4）: 245-252.
[6]	Chiappini E, Fossi F, Bonsignori F, et al. Utility of interferon-gamma release assay results to monitor anti-tubercular treatment in adults and children[J]. Clin Ther, 2012, 34（5）: 1041-1048. doi: 10.1016/j.clinthera.2012.03.006
[7]	汪锴, 张小舟, 康嗣如, 等. T-SPOT.TB检测在脊柱结核诊断及疗效评估中的价值[J]. 检验医学与临床, 2018, 10（15）: 2909-2912.
[8]	何紫琪, 李从荣. 结核感染T细胞斑点试验对结核病治疗效果的监测价值[J]. 海南医学, 2016, 27（12）: 1923-1925. doi: 10.3969/j.issn.1003-6350.2016.12.009
[9]	张腊红, 洪理泉, 罗贤, 等. 结核分枝杆菌相关IFN-γ释放试验在结核病诊断及疗效监测中的应用[J]. 放射免疫学杂志, 2013, 26（6）: 760-764. doi: 10.3969/j.issn.1008-9810.2013.06.039
[10]	任富贵. T细胞斑点试验在肺结核诊断和疗效评价中的意义[J]. 中国药药指南, 2018, 16（35）: 93-94.
[11]	陶学芳, 王华钧, 王建华, 等. T细胞斑点试验在肺结核诊断和疗效评价中的意义[J]. 中华临床感染病杂志, 2012, 5（4）: 221-224. doi: 10.3760/cma.j.issn.1674-2397.2012.04.007
[12]	李晓非, 刘红伟, 罗风, 等. T-SPOT.TB在肺结核早期抗痨治疗有效过程中的变化趋势研究[J]. 天津医药, 2019, 47（10）: 1062-1066.
[13]	Aiken AM, Hill PC, Fox A, et al. Reversion of the ELISPOT test after treatment in Gambian tuberculosis cases[J]. BMC Infect Dis, 2006, 6: 66. doi: 10.1186/1471-2334-6-66. doi: 10.1186/1471-2334-6-66
[14]	Tom G, Mary-Ann, Christine, et al. Reversion and conversion of mycobacterium tuberculosis IFN-γ ELISpot results during anti-tuberculous treatment in HIV-infected children[J]. BMC Infect Dis. 2010; 10: 138. doi: 10.1186/1471-2334-10-138

Relative Articles(20)

Relative Articles

[1]	Zhenxing YUAN, Wenfei SUN, Weidong WANG. Relationship between hs-CRP，CCL2，CCL3 and Neonatal Bacterial Infection and Their Predictive Value for Efficacy. Journal of Kunming Medical University, 2024, 45(7): 119-125. doi: 10.12259/j.issn.2095-610X.S20240718
[2]	Jihong LIU, Zhiqiang MA, Lin WANG, Xiaoyan WANG, Weiguang TIAN, Baoping LI, Rui DONG, Yujia LV. Correlation Analysis of Immune Function of Tuberculosis Patients Infected with COVID-19. Journal of Kunming Medical University, 2024, 45(4): 157-162. doi: 10.12259/j.issn.2095-610X.S20240423
[3]	Nihong LU, Honglu LIU, Yangjun CHEN, Yaping SUN, Yan YANG, Yongrui YANG, Jie LI. The Association between ESAT-6 Combined Multiple Detection Indicators and the Severity of Anti-tuberculosis Drug-induced Liver Injury. Journal of Kunming Medical University, 2024, 45(8): 52-57. doi: 10.12259/j.issn.2095-610X.S20240808
[4]	Xiaofeng ZHU, Guangping ZHOU, Laicang WANG, Houjun ZHOU, Xin GENG, Hualin YU, Jinghui LI, Peng BAI. Clinical Effect of Reoperation for Recurrent Glioma. Journal of Kunming Medical University, 2022, 43(9): 77-83. doi: 10.12259/j.issn.2095-610X.S20220920
[5]	Jin-hong LI, Hong WANG. Efficacy and Safety of Valsartan Amlodipine Tablets Combined with Alpha-lipoic Acid in the Treatment of Elderly Patients with Type 2 Diabetic Nephropathy Complicated with Hypertension. Journal of Kunming Medical University, 2021, 42(4): 53-56. doi: 10.12259/j.issn.2095-610X.S20210410
[6]	Na LI, Jie CHEN, Ji LUO, Yong-gang CHEN, Jiang-li PENG, Ming-li YU, Sheng-hao LI. Retrospective Analysis of 376 Cases of Adverse Reactions to Antituberculosis Drug. Journal of Kunming Medical University, 2021, 42(4): 117-121. doi: 10.12259/j.issn.2095-610X.S20210422
[7]	Zhe GE, Si-ping LU, Shi-zhong FU, Chun-hong WANG, Shao-ying LI, Ao LIU. The Preliminary Application of Whole Blood Interferon-Gamma Release Assay Combined with T Lymphocyte Subset Detection in Diagnosis of Pulmonary Tuberculosis. Journal of Kunming Medical University, 2021, 42(9): 108-113. doi: 10.12259/j.issn.2095-610X.S20210901
[8]	Ran Hai Liang , Yang Qing Huan , Chen Ying , Xiong Li Fen , Deng Yi Yu , He Xiu Yun . . Journal of Kunming Medical University, 2020, 41(06): 46-50.
[9]	Xing LIU, Bing OUYANG, Na LI, Ji LUO, Xu-qin SHI, Jie CHEN, Hui SUN, Hai-wen LI. Effect and Economic Evaluation of the Combination of Traditional Chinese and Western Medicine in Treatment of MDR-TB. Journal of Kunming Medical University, 2020, 41(11): 49-55. doi: 10.12259/j.issn.2095-610X.S20201106
[10]	Wan Rong , Li Ming Wu , Li Guang Mei , Zhu Hui Qiong , Ma Meng , Liu Cai . . Journal of Kunming Medical University, 2020, 41(09): 102-106.
[11]	Zhang Le , Xia Jia Wei , Chen Hai Yun , Huang Ying . . Journal of Kunming Medical University, 2018, 39(11): 93-97.
[12]	Sun Yu Peng , Zhang Chun Qiang , Du Kai Li , He Shao Bo , Guo Pei Yu , Zhao Run Min . The Value of T-SPOT.TB in Evaluating Treatment Effect of Tuberculous Vertebral Osteomyelitis. Journal of Kunming Medical University, 2018, 39(06): 86-90.
[13]	Liu Li , Wang Ya Ling , Bai Jing Song , Wang Li Xi , Wang Wei Bo , Chen Xiao Juan . Application of Cluster Nursing in Mechanical Ventilation Treatment of HIV Infection Complicated with Pulmonary Tuberculosis. Journal of Kunming Medical University, 2017, 38(07): 147-150.
[14]	Hong Xu Hua . Effects of Tuberculosis Drugs Combined with Rifapentine on the Immune Function， Inflammatory Factors， VEGF and MMP-9 of TB Patients. Journal of Kunming Medical University,
[15]	Zhang Wen Yan . . Journal of Kunming Medical University,
[16]	Tang Shi Cong . . Journal of Kunming Medical University,
[17]	Lu Ni Hong . . Journal of Kunming Medical University,
[18]	Lu Ni Hong . . Journal of Kunming Medical University,
[19]	Lu Ni Hong . Clinical Characteristics of Pulmonary Tuberculosis Patients with and without HIV(+)/AIDS. Journal of Kunming Medical University,
[20]	. The Value of T Cells Spots Test in the Diagnosis of Human Immunodeficiency Virus Amalgamative Tuberculosis Pateints with Different Levels of T cells. Journal of Kunming Medical University,