Determination of Five Nucleosides in <i>Periplaneta Americana</i> L. Extract by HPLC

Cong-qi ZHAO; Shun-bo YANG; Guang-da LI; Qi-hua LI; Yu-peng LI; Jun-ju XU

doi:10.12259/j.issn.2095-610X.S20210225

Volume 42 Issue 2

Mar. 2021

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Cong-qi ZHAO, Shun-bo YANG, Guang-da LI, Qi-hua LI, Yu-peng LI, Jun-ju XU. Determination of Five Nucleosides in Periplaneta Americana L. Extract by HPLC[J]. Journal of Kunming Medical University, 2021, 42(2): 13-17. doi: 10.12259/j.issn.2095-610X.S20210225

Citation:

Cong-qi ZHAO, Shun-bo YANG, Guang-da LI, Qi-hua LI, Yu-peng LI, Jun-ju XU. Determination of Five Nucleosides in Periplaneta Americana L. Extract by HPLC[J]. Journal of Kunming Medical University, 2021, 42(2): 13-17. doi: 10.12259/j.issn.2095-610X.S20210225

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Determination of Five Nucleosides in Periplaneta Americana L. Extract by HPLC

doi: 10.12259/j.issn.2095-610X.S20210225

1.
Colledge of Tobacco Science，Yunnan Agricultural University，Kunming Yunnan 650201
2.
College of Animal Science and Technology Yunnan Agricultural University，Kunming Yunnan 650201
3.
School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology of Natural Products，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2020-11-27
Publish Date: 2021-03-05

Abstract

Abstract

Objective To determine the content of five nucleosides in Periplaneta Americana L. extract by HPLC. Methods We did degreasing the Periplaneta Americana L.powder with petroleum ether, ultrasonic extraction of 65% ethanol as solvent, acetonitrile dissolution formulated into the American dating test solution, The Agilent alkyl silica gel column （9.4 mm × 250 mm, 5 μm） was applied in HPLC determination with water and acetonitrile mixture （95∶5） as the mobilephase at the flow rate of 0.5 mL·min⁻¹. Detection wavelength was set at 254 nm, 10 μL sample was injected for analysis. Results The determination of five nucleosides in Periplaneta Americana L. extract by HPLC with high sensitivity, special properties, could be used for the content determination of this product. The concentration of 5 kinds of nucleoside in a certain range and peak area showed a good linear relationship （R² > 0.9941）, high precision, good repeatability, good stability, plus sample recovery rate is between 97.58% to 103.26%. Conclusion The experimental process of this method is simple, the results are reliable and stable, and it can be used as a method for determining the content of nucleoside components in Periplaneta Americana L.
- Periplaneta Americana L,
- Nucleoside components,
- HPLC

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References(19)

References

[1]	清·孙星衍辑. 神农本草经[M] 第2卷. 北京: 商务印书馆, 1955: 576.
[2]	于珊珊,张华,张婷婷,等. 美洲大蠊药理作用及临床应用研究[J]. 辽宁中医药大学学报,2016,18(04):228-231.
[3]	戴云,曾茗,项朋志. 蜚蠊的药用价值[J]. 中药材,2005,28(9):848. doi: 10.3321/j.issn:1001-4454.2005.09.048
[4]	何正春,彭芳,宋丽艳,等. 美洲大蠊化学成分及药理作用研究进展[J]. 中国中药杂志,2007(21):2326. doi: 10.3321/j.issn:1001-5302.2007.21.031
[5]	李奇娟,王战国,刘巧,等. 美洲大蠊研究现状及其研究中关键问题分析与展望[J]. 中国中药杂志,2018,43(7):1507-1516.
[6]	Domínguez-Alvarez J,Mateos-Vivas M,Rodríguez Gonzalo E,et al. Determination of nucleosides and nucleotides in food samples by using liquid chromatography and capillary electrophoresis[J]. Trac Trends Anal Chem,2017,92(3):12-31.
[7]	李远辉. 康复新胃漂浮片的药学研究[D]. 成都: 成都中医药大学硕士论文, 2015.
[8]	蔡东,商琳琳,贾云宏,等. 胞嘧啶的合成研究进展[J]. 化工中间体,2009,5(2):20-23.
[9]	刘丽芳,金蓉鸾. HPLC法测定10种动物药中尿嘧啶,黄嘌呤,次黄嘌呤,脲苷的含量[J]. 中国中药杂志,1999,24(2):73-77. doi: 10.3321/j.issn:1001-5302.1999.02.003
[10]	刘国生,王玉娟,黄倩,等. 肌苷发酵过程腺嘌呤含量变化规律[J]. 食品科学,2013,34(15):194-197. doi: 10.7506/spkx1002-6630-201315040
[11]	何逵夫,马跃超,杜姗姗,等. 解淀粉芽胞杆菌关键酶基因过表达对鸟苷积累的影响[J]. 微生物学报,2012,52(6):718-725.
[12]	黄博,敬勇,张秀娟,等. HPLC法测定美洲大蠊药材中尿嘧啶、次黄嘌呤及肌苷的含量[J]. 中药材,2014,37(9):1537-1540.
[13]	罗廷顺,高孟婷,马芳芳,等. 美洲大蠊药理作用及临床应用研究进展[J]. 安徽农业科学,2012,40(10):219-221.
[14]	胡艳芬,吕小满,王玉梅,等. 美洲大蠊药用价值研究进展[J]. 医学综述,2008,14(18):2822-2824.
[15]	尹卫平,姜亚玲,李鹏飞,等. 昆虫美洲大蠊天然产物的研究及其化学分类学意义[J]. 河南科技大学学报:自然科学版,2012,33(5):101-104.
[16]	黄兰芳,郭方遒,梁逸曾,等. HPLC ESI-MS 测定冬虫夏草和蚕蛹虫草中腺苷和虫草素含量[J]. 中国中药杂志,2004,29(8):762. doi: 10.3321/j.issn:1001-5302.2004.08.015
[17]	马艳,汪宇,杨光照,等. 双波长薄层扫描法测定虫草类制剂中核苷类化合物的含量[J]. 中国药房,2008,19(30):2375.
[18]	李泳,余潮彬,张兆霞,等. 紫外可见分光光度法测定虫草素含量[J]. 广东化工,2012,39(11):186. doi: 10.3969/j.issn.1007-1865.2012.11.101
[19]	李静,朱静毅. 核苷类化合物含量测定方法的研究进展[J]. 天津药学,2013,25(6):53-55.

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