Establishment of Rat Myoblast Atrophy Model Induced by Ang Ⅱ

Wei-jie SU; Mei-sha ZHANG; Jia-ning LI; Ying LIU; Ding-jie XU; Hong CHANG

doi:10.12259/j.issn.2095-610X.S20211026

Volume 42 Issue 10

Oct. 2021

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Wei-jie SU, Mei-sha ZHANG, Jia-ning LI, Ying LIU, Ding-jie XU, Hong CHANG. Establishment of Rat Myoblast Atrophy Model Induced by Ang Ⅱ[J]. Journal of Kunming Medical University, 2021, 42(10): 8-13. doi: 10.12259/j.issn.2095-610X.S20211026

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Wei-jie SU, Mei-sha ZHANG, Jia-ning LI, Ying LIU, Ding-jie XU, Hong CHANG. Establishment of Rat Myoblast Atrophy Model Induced by Ang Ⅱ[J]. Journal of Kunming Medical University, 2021, 42(10): 8-13. doi: 10.12259/j.issn.2095-610X.S20211026

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Establishment of Rat Myoblast Atrophy Model Induced by Ang Ⅱ

doi: 10.12259/j.issn.2095-610X.S20211026

Traditional Chinese Medicine College，North China University of Science and Technology，Tangshan Hebei 063210，China

Received Date: 2021-08-04
Available Online: 2021-10-29
Publish Date: 2021-10-30

Abstract

Abstract

Objective To provide a model basis for further study of heart failure complicated with sarcopenia, a rat myoblast （L6） atrophy model induced by Ang Ⅱ was established. Methods 2% horse serum was used to induce differentiation of L6 cells. The time of induced differentiation was determined by microscopic observation and MyHC protein expression. CCK-8 was used to detect the effects of different concentrations of Ang Ⅱ on L6 cells. To determine the conditions of the cell atrophy model, different concentrations of Ang Ⅱ were selected to treat L6 cells, and the expression of MyHC protein was detected by Western Blot and immunofluorescence. Results L6 cells were induced to differentiate successfully after growing in 2% horse serum for 6 days. Microscopic observation revealed the fusion and myotubule of L6 cells, and the expression of MyHC protein was also increased. Ang Ⅱ at 0.1 μm to 10 μm had no effect on the activity of L6 cells. Western Blot and immunofluorescence detection showed that the MyHC protein expression level decreased after treated with 5 and 10 μM Ang Ⅱ for 72 h. Conclusions The model of L6 cell atrophy can be established by the treatment with 10 μM Ang Ⅱ for 72 h. This model will provide the basis for in vitro cell study of heart failure complicated with sarcopenia.
- L6 cell,
- Heart failure,
- Sarcopenia,
- Skeletal muscle atrophy model

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