Volume 42 Issue 10
Oct.  2021
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Wei-jie SU, Mei-sha ZHANG, Jia-ning LI, Ying LIU, Ding-jie XU, Hong CHANG. Establishment of Rat Myoblast Atrophy Model Induced by Ang Ⅱ[J]. Journal of Kunming Medical University, 2021, 42(10): 8-13. doi: 10.12259/j.issn.2095-610X.S20211026
Citation: Wei-jie SU, Mei-sha ZHANG, Jia-ning LI, Ying LIU, Ding-jie XU, Hong CHANG. Establishment of Rat Myoblast Atrophy Model Induced by Ang Ⅱ[J]. Journal of Kunming Medical University, 2021, 42(10): 8-13. doi: 10.12259/j.issn.2095-610X.S20211026

Establishment of Rat Myoblast Atrophy Model Induced by Ang Ⅱ

doi: 10.12259/j.issn.2095-610X.S20211026
  • Received Date: 2021-08-04
    Available Online: 2021-10-29
  • Publish Date: 2021-10-30
  •   Objective  To provide a model basis for further study of heart failure complicated with sarcopenia, a rat myoblast (L6) atrophy model induced by Ang Ⅱ was established.   Methods  2% horse serum was used to induce differentiation of L6 cells. The time of induced differentiation was determined by microscopic observation and MyHC protein expression. CCK-8 was used to detect the effects of different concentrations of Ang Ⅱ on L6 cells. To determine the conditions of the cell atrophy model, different concentrations of Ang Ⅱ were selected to treat L6 cells, and the expression of MyHC protein was detected by Western Blot and immunofluorescence.   Results  L6 cells were induced to differentiate successfully after growing in 2% horse serum for 6 days. Microscopic observation revealed the fusion and myotubule of L6 cells, and the expression of MyHC protein was also increased. Ang Ⅱ at 0.1 μm to 10 μm had no effect on the activity of L6 cells. Western Blot and immunofluorescence detection showed that the MyHC protein expression level decreased after treated with 5 and 10 μM Ang Ⅱ for 72 h.   Conclusions  The model of L6 cell atrophy can be established by the treatment with 10 μM Ang Ⅱ for 72 h. This model will provide the basis for in vitro cell study of heart failure complicated with sarcopenia.
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