Effects of Scutellarin on Expressions of PKC and TNF-α in OX-LDL Injured RAW264.7 Cells <i>in vitro</i>

Li ZHANG; Dan YU; Yu-ting WANG; Zhi-mei GOU; Ren-hua YANG; Bo HE; Zhi-qiang SHEN; Wen-ying CHAI; Peng CHEN

doi:10.12259/j.issn.2095-610X.S20211040

Volume 42 Issue 10

Oct. 2021

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Li ZHANG, Dan YU, Yu-ting WANG, Zhi-mei GOU, Ren-hua YANG, Bo HE, Zhi-qiang SHEN, Wen-ying CHAI, Peng CHEN. Effects of Scutellarin on Expressions of PKC and TNF-α in OX-LDL Injured RAW264.7 Cells in vitro[J]. Journal of Kunming Medical University, 2021, 42(10): 1-7. doi: 10.12259/j.issn.2095-610X.S20211040

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Effects of Scutellarin on Expressions of PKC and TNF-α in OX-LDL Injured RAW264.7 Cells in vitro

doi: 10.12259/j.issn.2095-610X.S20211040

1.
School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products，Kunming Medical University，Kunming Yunnan 650500
2.
Dept. of Breast Surgery，First Affiliated Hospital of Kunming Medical University， Kunming Yunnan 650031，China

Received Date: 2021-07-12
Available Online: 2021-10-29
Publish Date: 2021-10-30

Abstract

Abstract

Objectives To investigate the effects of scutellarin on lipid phagocytosis and the regulation of PKC, and TNF-α expressions in low density lipoprotein （OX-LDL） injured RAW264.7 cells. Methods Murine RAW264.7 macrophages was cultured in vitro. Then, the OX-LDL-induced injury model was replicated. Combined with oil red O staining, cellular cholesteryl ester content was also detected in OX-LDL-induced injury model. Furthermore, Western blot and RT-PCR methods were used to detect changes in PKC and TNF-α protein as well as mRNA expression in RAW264.7 cells. Results （1） The results of oil red O showed that the degree of cell red staining in model group was significantly higher than that in normal control group; compared with model group, the degree of cell red staining in simvastatin group, scutellarin 50 and 100 μmol/L group were lower than that in the model group; （2） The cholesteryl ester content assay showed that: compared with the normal control group, the CE/TC values of the model group cells were significantly increased at each time point; compared with model group, and cellular CE/TC values were significantly decreased in the dosing group at each time point. （3） WB and RT-PCR results showed that compared with normal control group, protein and mRNA expressions of PKC and TNF-α were increased in the model group; compared with the model group, protein and mRNA expression of PKC and TNF-α were decreased in simvastatin group, and scutellarin group showed a concentration-dependent downregulation of PKC and TNF-α expression. Conclusions （1） Scutellarin can significantly inhibit the levels of cholesterol in OX-LDL injuried RAW264.7 cells. （2） Scutellarin can down-regulated the protein and mRNA expressions of PKC, TNF-α in OX-LDL induced RAW264.7 cells.
- Scutellarin,
- RAW264.7 macrophages,
- oxidized low-density lipoprotein （OX-LDL）,
- PKC,
- TNF-α

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References

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