Volume 42 Issue 10
Oct.  2021
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Li ZHANG, Dan YU, Yu-ting WANG, Zhi-mei GOU, Ren-hua YANG, Bo HE, Zhi-qiang SHEN, Wen-ying CHAI, Peng CHEN. Effects of Scutellarin on Expressions of PKC and TNF-α in OX-LDL Injured RAW264.7 Cells in vitro[J]. Journal of Kunming Medical University, 2021, 42(10): 1-7. doi: 10.12259/j.issn.2095-610X.S20211040
Citation: Li ZHANG, Dan YU, Yu-ting WANG, Zhi-mei GOU, Ren-hua YANG, Bo HE, Zhi-qiang SHEN, Wen-ying CHAI, Peng CHEN. Effects of Scutellarin on Expressions of PKC and TNF-α in OX-LDL Injured RAW264.7 Cells in vitro[J]. Journal of Kunming Medical University, 2021, 42(10): 1-7. doi: 10.12259/j.issn.2095-610X.S20211040

Effects of Scutellarin on Expressions of PKC and TNF-α in OX-LDL Injured RAW264.7 Cells in vitro

doi: 10.12259/j.issn.2095-610X.S20211040
  • Received Date: 2021-07-12
    Available Online: 2021-10-29
  • Publish Date: 2021-10-30
  •   Objectives  To investigate the effects of scutellarin on lipid phagocytosis and the regulation of PKC, and TNF-α expressions in low density lipoprotein (OX-LDL) injured RAW264.7 cells.   Methods  Murine RAW264.7 macrophages was cultured in vitro. Then, the OX-LDL-induced injury model was replicated. Combined with oil red O staining, cellular cholesteryl ester content was also detected in OX-LDL-induced injury model. Furthermore, Western blot and RT-PCR methods were used to detect changes in PKC and TNF-α protein as well as mRNA expression in RAW264.7 cells.   Results  (1) The results of oil red O showed that the degree of cell red staining in model group was significantly higher than that in normal control group; compared with model group, the degree of cell red staining in simvastatin group, scutellarin 50 and 100 μmol/L group were lower than that in the model group; (2) The cholesteryl ester content assay showed that: compared with the normal control group, the CE/TC values of the model group cells were significantly increased at each time point; compared with model group, and cellular CE/TC values were significantly decreased in the dosing group at each time point. (3) WB and RT-PCR results showed that compared with normal control group, protein and mRNA expressions of PKC and TNF-α were increased in the model group; compared with the model group, protein and mRNA expression of PKC and TNF-α were decreased in simvastatin group, and scutellarin group showed a concentration-dependent downregulation of PKC and TNF-α expression.   Conclusions  (1) Scutellarin can significantly inhibit the levels of cholesterol in OX-LDL injuried RAW264.7 cells. (2) Scutellarin can down-regulated the protein and mRNA expressions of PKC, TNF-α in OX-LDL induced RAW264.7 cells.
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