Studies on Cisplatin Resistance of miR-181a in Ovarian Cancer Cells

Lixiu XU; TUERXUN Meiliban; YAKUFU Kereman

doi:10.12259/j.issn.2095-610X.S20220131

Volume 43 Issue 1

Jan. 2022

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Lixiu XU, TUERXUN Meiliban, YAKUFU Kereman. Studies on Cisplatin Resistance of miR-181a in Ovarian Cancer Cells[J]. Journal of Kunming Medical University, 2022, 43(1): 20-25. doi: 10.12259/j.issn.2095-610X.S20220131

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Lixiu XU, TUERXUN Meiliban, YAKUFU Kereman. Studies on Cisplatin Resistance of miR-181a in Ovarian Cancer Cells[J]. Journal of Kunming Medical University, 2022, 43(1): 20-25. doi: 10.12259/j.issn.2095-610X.S20220131

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Studies on Cisplatin Resistance of miR-181a in Ovarian Cancer Cells

doi: 10.12259/j.issn.2095-610X.S20220131

1.
Basic Medicine College of Xinjiang Medical University，Urumqi Xinjiang，830011
2.
Dept. of Gynecology People’s Hospital of Xinjiang Uygur Autonomous Region，Urumqi Xinjiang，830001

Received Date: 2021-12-26
Available Online: 2021-12-20
Publish Date: 2022-01-15

Abstract

Abstract

Objective To investigate the effect of miR-181a on chemotherapy resistance of ovarian cancer and its regulatory mechanism. Methods Real-time PCR was used to detect the expression of miR-181a in A2780 and cisplatin resistant cell lines （A2780/DDP）, and siltation/overexpression changed the expression of miR-181a in A2780 and A2780/DDP cells. MTT method was used to determine the sensitivity of cells to cisplatin before and after the transfection. Three MicroRNA target gene databases, TargetScan, miRDB and miRwalk, were used to predict the downstream targets of miR-181a, and the changes in protein expression of target genes were analyzed by Western blot. Results Compared with A2780 cells, the expression of miR-181a in A2780/DDP cells was significantly decreased （P < 0.05）. After the transfection of A2780 cells with miR-181a inhibitor （interfering with Mir-181A expression）, the sensitivity of A2780 cells to cisplatin decreased （P < 0.05）, and after the transfection of A2780/DDP cells with miR-181a mimic （overexpressing miR-181a）, The sensitivity of cells to cisplatin was increased （P < 0.05）. It was predicted that PRKCD could be used as the downstream target gene of miR-181a by TargetScan, miRDB and miRwalk databases, and down-regulation of miR-181a expression could significantly enhance PRKCD protein expression （P < 0.05）. On the contrary, up-regulation of miR-181a significantly inhibited PRKCD protein expression （P < 0.05）. Conclusion miR-181a may inhibit cisplatin resistance of ovarian cancer cell A2780 by regulating PRKCD.
- Ovarian cancer,
- PRKCD,
- miR-181a,
- Cisplatin resistance

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References(26)

References

[1]	Amjadi M,Hallaj T,Hildebrandt N. A sensitive homogeneous enzyme assay for euchromatic histone-lysine-N-methyltransferase 2 (G9a) based on terbium-to-quantum dot time-resolved FRET[J]. Bioimpacts,2021,11(3):173-179.
[2]	Giordano M,Decio A,Battistini C,et al. L1CAM promotes ovarian cancer stemness and tumor initiation via FGFR1/SRC/STAT3 signaling[J]. J ExpClin Cancer Res,2021,40(1):319.
[3]	Zhang X,Qin T,Zhu Z,et al. Ivermectin augments the in vitro and in vivo efficacy of cisplatin in epithelial ovarian cancer by suppressing Akt/mTOR signaling[J]. Am J Med Sci,2020,359(2):123-129.
[4]	Hu Z,Cai M,Zhang Y,et al. miR-29c-3p inhibits autophagy and cisplatin resistance in ovarian cancer by regulating FOXP1/ATG14 pathway[J]. Cell Cycle (Georgetown,Tex. ),2020,19(2):193-206.
[5]	Forouhari S,Mahmoudi E,Safdarian E,et al. MicroRNA:A Potential Diagnosis for Male Infertility[J]. Mini Rev Med Chem,2021,21(10):1226-1236. doi: 10.2174/1389557520999201209213319
[6]	Kim J,You S. Samul-tang ameliorates oocyte damage due to cyclophosphamide-induced chronic ovarian dysfunction in mice[J]. Sci Rep,2020,10(1):21925. doi: 10.1038/s41598-020-79013-7
[7]	Jacob H,Stanisavljevic L,Storli K E,et al. Identification of a sixteen-microRNA signature as prognostic biomarker for stage II and III colon cancer[J]. Oncotarget,2017,8(50):87837-87847. doi: 10.18632/oncotarget.21237
[8]	Huang F L,Liao E C,Li C L,et al. Pathogenesis of pediatric B-cell acute lymphoblastic leukemia: Molecular pathways and disease treatments[J]. Oncol Lett,2020,20(1):448-454. doi: 10.3892/ol.2020.11583
[9]	Al-Hazmi A. Antioxidant activity of silymarin in microcystin-LR cardiac and pulmonary induced injuries on mice[J]. Pak J Biol Sci,2020,23(11):1369-1373. doi: 10.3923/pjbs.2020.1369.1373
[10]	Lei B,Huang Y,Zhou Z,et al. Circular RNA hsa_circ_0076248 promotes oncogenesis of glioma by sponging miR-181a to modulate SIRT1 expression[J]. Cell Biochem,2019,120(4):6698-6708. doi: 10.1002/jcb.27966
[11]	Abramson E,Hardman C,Shimizu A J,et al. Designed PKC-targeting bryostatin analogs modulate innate immunity and neuroinflammation[J]. Cell ChemBiol,2021,28(4):537-545.e4.
[12]	Ning Li,Long Bo,Zhang Wen,et al. Circular RNA profiling reveals circEXOC6B and circN4BP2L2 as novel prognostic biomarkers in epithelial ovarian cancer[J]. Int J Oncol,2018,53(6):2637-2646.
[13]	Giampaolino P,Della Corte L,Foreste V,et al. Unraveling a difficult diagnosis: The tricks for early recognition of ovarian cancer[J]. Minerva Med,2019,110(4):279-291.
[14]	Ni J,Zhang X,Li J,et al. Tumour-derived exosomal lncRNA-SOX2OT promotes bone metastasis of non-small cell lung cancer by targeting the miRNA-194-5p/RAC1 signalling axis in osteoclasts[J]. Cell Death Dis,2021,12(12):1131.
[15]	Ahn J H,Lee H S,Lee J S,et al. Nc886 is induced by TGF-β and suppresses the microRNA pathway in ovarian cancer[J]. Nat Commun,2018,9(1):1166. doi: 10.1038/s41467-018-07818-2
[16]	王皓,周宇. 微小RNA在肝胆胰疾病中作用的研究进展[J]. 实用肝脏病杂志,2011,14(2):155-157. doi: 10.3969/j.issn.1672-5069.2011.02.033
[17]	De Paula Silva E,Marti L C,Andreghetto F M,et al. Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs[J]. Sci Rep,2021,11(1):18534. doi: 10.1038/s41598-021-97753-y
[18]	Gao Jianlong,Xia Silong. Reduced miR-519d-3p levels in the synovium and synovial fluid facilitate the progression of post-traumatic osteoarthritis by targeting VEGF[J]. Exp Ther Med,2021,22(6):1478. doi: 10.3892/etm.2021.10913
[19]	陈怡然. MicroRNA-181a靶向PRKCD调节子宫颈鳞癌化疗抵抗性的机制研究[D]. 上海: 复旦大学博士学位论文, 2013.
[20]	Ben-Mustapha I,Agrebi N,Barbouche M R. Novel insights into FAS defects underlying autoimmune lymphoproliferative syndrome revealed by studies in consanguineous patients[J]. J Leukoc Biol,2018,103(3):501-508.
[21]	Muscella A,Cossa L G,Vetrugno C,et al. Inhibition of ZL55 cell proliferation by ADP via PKC-dependent signalling pathway[J]. J Cell Physiol,2018,233(3):2526-2536. doi: 10.1002/jcp.26128
[22]	Omarjee O,Picard C,Frachette C,et al. Monogenic lupus:Dissecting heterogeneity[J]. Autoimmun Rev,2019,18(10):102361. doi: 10.1016/j.autrev.2019.102361
[23]	Munson M J,Mathai B J,Ng M Y W,et al. GAK and PRKCD are positive regulators of PRKN-independent mitophagy[J]. Nat Commun,2021,12(1):6101. doi: 10.1038/s41467-021-26331-7
[24]	Chun K H,Cho S J,Lee J W,et al. Protein kinase C-δ interacts with and phosphorylates ARD1[J]. J Cell Physiol,2021,236(1):379-391. doi: 10.1002/jcp.29866
[25]	Lothstein L,Soberman J,Parke D,et al. Pivarubicin is more effective than doxorubicin against triple-negative breast cancer in vivo[J]. Oncol Res,2020,28(5):451-465. doi: 10.3727/096504020X15898794315356
[26]	Chen Y,Ke G,Han D,et al. MicroRNA-181a enhances the chemoresistance of human cervical squamous cell carcinoma to cisplatin by targeting PRKCD[J]. Exp Cell Res,2014,320(1):12-20. doi: 10.1016/j.yexcr.2013.10.014

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