The Relationship Between T950C Polymorphism in the Promoter Region of Osteoprotegerin Gene and Type 2 Diabetes with Osteoporosis

Boyi LI; Ling NIU; Rong MA; Xian ZHANG; Fang LIU; Yan TANG; Cuijuan MIAO; Cheng ZHANG; Zhujun HAN

doi:10.12259/j.issn.2095-610X.S20220306

Volume 43 Issue 3

Mar. 2022

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Article Navigation > Journal of Kunming Medical University > 2022 > 43(3): 67-73

Boyi LI, Ling NIU, Rong MA, Xian ZHANG, Fang LIU, Yan TANG, Cuijuan MIAO, Cheng ZHANG, Zhujun HAN. The Relationship Between T950C Polymorphism in the Promoter Region of Osteoprotegerin Gene and Type 2 Diabetes with Osteoporosis[J]. Journal of Kunming Medical University, 2022, 43(3): 67-73. doi: 10.12259/j.issn.2095-610X.S20220306

Citation:

Boyi LI, Ling NIU, Rong MA, Xian ZHANG, Fang LIU, Yan TANG, Cuijuan MIAO, Cheng ZHANG, Zhujun HAN. The Relationship Between T950C Polymorphism in the Promoter Region of Osteoprotegerin Gene and Type 2 Diabetes with Osteoporosis[J]. Journal of Kunming Medical University, 2022, 43(3): 67-73. doi: 10.12259/j.issn.2095-610X.S20220306

Citation:

Boyi LI, Ling NIU, Rong MA, Xian ZHANG, Fang LIU, Yan TANG, Cuijuan MIAO, Cheng ZHANG, Zhujun HAN. The Relationship Between T950C Polymorphism in the Promoter Region of Osteoprotegerin Gene and Type 2 Diabetes with Osteoporosis[J]. Journal of Kunming Medical University, 2022, 43(3): 67-73. doi: 10.12259/j.issn.2095-610X.S20220306

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The Relationship Between T950C Polymorphism in the Promoter Region of Osteoprotegerin Gene and Type 2 Diabetes with Osteoporosis

doi: 10.12259/j.issn.2095-610X.S20220306

Dep. of Endocrinology，Kunming First People’s Hospital，Kunming Yunnan 650011，China

Received Date: 2022-02-14
Publish Date: 2022-03-25

Abstract

Abstract

Objective To investigate the relationship between T950C polymorphism in the promoter region of osteoprotegerin （OPG） gene and type 2 diabetes mellitus （T2DM） complicated with osteoporosis, and to understand the genetically related susceptibility genes of T2DM complicated with osteoporosis. Methods （1） Using polymerase chain reaction technology, the T2DM without osteoporosis group （group A）, the T2DM with osteopenia group （B group）, the T2DM with osteoporosis group （C group）, the OPG gene T950C group were detected. Genotype, the OPG gene T950C polymorphism in the three groups was analyzed respectively; （2） Gender, body mass index, age, blood pressure, weight, glycated hemoglobin, height, blood lipids, fasting blood glucose, testosterone, estrogen, vitamin D were compared among the three groups. （3） The differences in bone mineral density was compared between different parts of the OPG gene T950C genotype; （4） The independent risk factors of T2DM patients with osteoporosis was learned through logistic regression analysis. Results （1） TC type was the main genotype of OPG gene T950C in T2DM and T2DM patients with osteoporosis; （2） There was no significant difference in the genotype frequency and allele frequency of OPG gene T950C among the three groups （P > 0.05）; （3） The weight, age, height, BMI, and BMI of the three groups of patients were compared, the difference was statistically significant （ P < 0.05）, and the differences among the groups were statistically significant （ P < 0.05）; （ 4） Comparison of the three genotypes of the OPG gene T950C, there was no difference in the bone mineral density of each part （ P > 0.05）; （5） Regression analysis showed that age and smoking entered the regression equation, but the genotype of the OPGOPG gene T950C did not enter regression equation. Conclusions （1） TC type is the main genotype of OPG gene T950C in T2DM and T2DM patients with osteoporosis; （2） The genotype of OPG gene T950C may not be a susceptibility gene for T2DM and T2DM combined with osteoporosis; （3） Smoking and age are independent risk factors of T2DM combined with osteoporosis.
- Osteoprotegerin （OPG）,
- Gene promoter region T950C,
- Polymorphism,
- Type 2 diabetic,
- Osteoporosis

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References(28)

References

[1]	中华医学会糖尿病学分会. 中国2型糖尿病防治指南(2020年版)[J]. 中华糖尿病杂志,2021,13(4):315-409.
[2]	中华医学会骨质疏松和骨矿盐疾病分会. 中国骨质疏松症流行病学调查及"健康骨骼" 专项行动结果发布[J]. 中华骨质疏松和骨矿盐疾病杂志,2019,12(4):317-318. doi: 10.3969/j.issn.1674-2591.2019.04.001
[3]	Rakel A,Sheehy O,Rahme E,LeLorier J. Osteoporosis among patients with type 1 and type 2 daibetes[J]. Diabetes Metabolism,2008,34(2):193-205. doi: 10.1016/j.diabet.2007.10.008
[4]	Franke S,Siggelkow H,Wolf G,et al. Advanced glycation endproducts influence the Mrna expression of RAGE,RANKL and various osteoblastic genes in humanosteoblasts[J]. Arch Physiol Biochen,2007,113(3):154-161. doi: 10.1080/13813450701602523
[5]	Botolin S,McCabe L R. Chronic hyperglycemia modulates osteoblast gene expression through osmotic and non-osmotic pathways[J]. J Cell Biochem,2006,99(2):411-417. doi: 10.1002/jcb.20842
[6]	GordonGong,Gleb Haynatzki,Vera Haynatzka,et al. Bone miberal density-affecting denes in Africans[J]. J The National Medical Association,2006,98(7):1102-1108.
[7]	中华医学会糖尿病学分会. 中国2型糖尿病防治指南[J]. 中国糖尿病杂志,2018,10(1):4-67.
[8]	中华医学会骨质疏松和骨矿盐分会. 原发性骨质疏松症诊治指南[J]. 中华骨质疏松和骨矿盐疾病杂志,2017,10(5):413-433.
[9]	中华中医药学会糖尿病分会. 糖尿病合并骨质疏松中医诊疗标准[J]. 世界中西医结合杂志,2011,6(3):265-269. doi: 10.3969/j.issn.1673-6613.2011.03.033
[10]	Vestergard P. Discrepancies in bone mineral density and fracture risk in patients with type 1 and type 2 diabetes -a meta-analysis[J]. Osteoporosis Int,2007,18(2):427-444.
[11]	Botolin S, McCabe L R. Chronic hyperglycemia modulates osteoblast gene expression through osmotic and non-osmotic pathways[J]. J Cell Biochem, 2006, 99（10）: 411-417.
[12]	曹国磊, 王思瑶, 李思源, 等. 老年男性2型糖尿病患者骨密度情况及相关影响因素, 中国老年学杂志, 2017, 2（37）: 612-613.
[13]	Zhu G Y,Wang H F,Shi Y X,et al. Environmengt eadmium exposure and forearm bone density[J]. Biometals,2007,17(5):499-503.
[14]	Akesson A,Bjiellerup P,Lundh T,et al. Cadmium-induced effects on bone in a population-based study of women[J]. Environ Health Per-spect,2006,114(6):830-834. doi: 10.1289/ehp.8763
[15]	赵金秀,周学瀛,刘国仰,等. 北京地区汉族人维生素D受体基因多态性分布[J]. 中国医学科学院学报,1997,19(1):18.
[16]	Arko B,Prezelj J,Komel R,et al. Sequence variations in theosteoprotegrin gene promoter in patients with postmenopausalosteoporosis[J]. J Clin Endocrinol Metab,2002,87(9):4080.
[17]	Zajícková K,Zemanová A,Hill M,et al. Is A163G polymorphism in theosteoprotegerin gene associated with heel velocity of sound inpostmenopausal women?[J]. Physiol Res,2008,57(Suppl 1):S153-S157.
[18]	Knudsen S T,Jeppesen P,Poulsen P L,et al. Plasma concentrations ofosteoprotegerin during normo-and hyperglycaemicclamping[J]. Seand J Clin Lab Invest,2007,67(2):135-142.
[19]	Suzuki K,Kurose T,Takizawa M,et al. Osteoclastic function is accelerated in male patients with type 2 diabetes mellitus:the preventive role of osteoclastogenesis inhibitory factor/osteoprotegerin (OCIF/OPG) on the decrease of bone mineral density[J]. Diabetes Res Clin Pract,2005,68(2):117-125.
[20]	郑蓉, 苗懿德, 纪立农等, 中国组织工程研究与临床康复[J]. 2011, 15（28）: 5292-5294.
[21]	刘杰,苗懿德,张育军,等. 绝经后妇女护骨素基因启动子区T950C基因多态性与骨密度的关系研究[J]. 中国骨质疏松杂志,2021,16(3):184-187.
[22]	武兆忠,刘敏,冯鉴强. 护骨素基因启动子区T950-C多态性与骨密度及骨代谢的关系[J]. 中国老年学杂志,2005,25(3):247-250. doi: 10.3969/j.issn.1005-9202.2005.03.007
[23]	刘宇鹏. 骨保护素基因多态性及骨质疏松型骨折的研究[D]. 大连: 大连理工大学博士学位论文, 2016.
[24]	Garciaunzueta M T,Riancho J A,Zarrabeitia M T,et al. Association of the 163A/G and 118G/C osteoprotegerin polymorphism with bone mineral density[J]. Hormone & Metabolic Research,2008,40(3):219-224.
[25]	Moffett S P,Oakley J I,Cauley J A,eta1. Osteoprotegerin Lys3Asn polymorphism and the riskof fracture in older women.[J]. Journal of Clinical Endocrinology & Metabolism,2008,93(5):2002-2008.
[26]	Tothill P,Laskey M C,Van W M. Anomalies in dual energy X-ray absorptiometry measurements of total—body bone mineral during weight change using Lunar. Hologic and Norland instruments.[J]. British Journal of Radiology,1999,72(859):661-669. doi: 10.1259/bjr.72.859.10624323
[27]	Woo J,Kim Y,Lee C. Heterogeneous genetic associations of nucleotide sequence variants with bone mineral density by gender[J]. Molecular Biology Reports,2012,39(3):2259-2265. doi: 10.1007/s11033-011-0975-z
[28]	Zhao H Y,Liu J M,Ning G,et a1. The influence of Lys3Asn Polymorphism in the osteoprotegerin gene on bone mineral density in Chinese postmenopausal women[J]. Osteoporosis International,2005,16(12):1519-1524. doi: 10.1007/s00198-005-1865-9

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