Analysis of Intestinal Flora and Therapeutic Intervention in Children with Functional Constipation

Sixiang LIU; Yongkun HUANG; Mingying WANG; Hongwei HU; Min MA; Yu LING

doi:10.12259/j.issn.2095-610X.S20220309

Volume 43 Issue 3

Mar. 2022

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Article Navigation > Journal of Kunming Medical University > 2022 > 43(3): 123-127

Sixiang LIU, Yongkun HUANG, Mingying WANG, Hongwei HU, Min MA, Yu LING. Analysis of Intestinal Flora and Therapeutic Intervention in Children with Functional Constipation[J]. Journal of Kunming Medical University, 2022, 43(3): 123-127. doi: 10.12259/j.issn.2095-610X.S20220309

Citation:

Sixiang LIU, Yongkun HUANG, Mingying WANG, Hongwei HU, Min MA, Yu LING. Analysis of Intestinal Flora and Therapeutic Intervention in Children with Functional Constipation[J]. Journal of Kunming Medical University, 2022, 43(3): 123-127. doi: 10.12259/j.issn.2095-610X.S20220309

Citation:

Sixiang LIU, Yongkun HUANG, Mingying WANG, Hongwei HU, Min MA, Yu LING. Analysis of Intestinal Flora and Therapeutic Intervention in Children with Functional Constipation[J]. Journal of Kunming Medical University, 2022, 43(3): 123-127. doi: 10.12259/j.issn.2095-610X.S20220309

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Analysis of Intestinal Flora and Therapeutic Intervention in Children with Functional Constipation

doi: 10.12259/j.issn.2095-610X.S20220309

1.
Dept. of Children Healthcare，The Affiliated Children Hospital of Kunming Medical University，Kunming Yunnan 650034
2.
Dept. of Pediatrics，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032
3.
Dept. of Gastroenterology，The Affiliated Children Hospital of Kunming Medical University，Kunming Yunnan 650034，China

Received Date: 2022-01-19
Available Online: 2022-02-18
Publish Date: 2022-03-22

Abstract

Abstract

Objective To analyze the changes of intestinal flora in children with functional constipation （FC） and the clinical significance of non-pharmacological basic treatment. Methods A total of 30 children with FC were selected from the outpatient department of the Children's Hospital affiliated to Kunming Medical University between September 2019-October 2021, and 30 health children were selected as control group. The children with FC were given basic treatment that including dietary structure adjustment, regular defecation training and appropriate exercise.The fecal samples were collected respectively in the pre-treatment group and post-treatment group from the children with FC and from the healthy children. The contents of intestinal flora in the stools of children in each group were measured by the MiNi8 plus fluorescence quantitative PCR instrument from the Kunming Hehe Medical Laboratory. In addition, the relief of constipation symptoms after basic treatment in children with FC was observed. Results The content of lactobacillus and bifidobacterium in children with FC was significantly lower than that in control group , and the difference was statistically significant （P < 0.01）. The content of lactobacillus in the post-treatment group was significantly higher than that in the pre-treatment group, and the difference was statistically significant （P < 0.01）. The scores of constipation symptoms in children with FC after basic treatment were lower than those in pre-treatment group, and the difference was statistically significant （P < 0.01）. Conclusions The children with FC will have intestinal flora disorder. After basic treatment such as dietary structure adjustment and lifestyle improvement, the intestinal flora disorder and constipation symptoms can be improved.
- Functional constipation,
- Intestinal flora,
- Basic treatment,
- Children

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References(21)

References

[1]	耿岚岚,刘明南,龙高,等. 儿童功能性胃肠病罗马Ⅳ标准[J]. 中华儿科杂志,2017,55(1):4-14. doi: 10.3760/cma.j.issn.0578-1310.2017.01.002
[2]	Koppen I J N,Vriesman M H,Saps M,et al. Prevalence of functional defecation disorders in children:A systematic review and meta-analysis[J]. J Pediatr,2018,198(1):121-130.
[3]	Loening-thucke V,Prevalence. Symptoms and outcome of consti-pation in infants and toddler[J]. J Pediatr,2005,146(3):359-363. doi: 10.1016/j.jpeds.2004.10.046
[4]	Rasquin-Weber A,Hyman P E,Cucehiara S,et al. Childhood funetional gastrointestinal disorders[J]. Gut,1999,45(suppl II):f1160-f1168.
[5]	张良娟,王宝西. 儿童功能性便秘相关检查的研究进展[J]. 临床儿科杂志,2014,32(6):587-590.
[6]	Gastillejo G,Bulló M,Anguera A,et al. A controlled,randomized,double‐blind trial to evaluate the effect of a supplement of cocoa husk that is rich in dietary fiber on colonic transit in constipated pediatric patients[J]. Pediatrics,2006,118(3):641‐648.
[7]	Bleijenberg G,Kuijpers H C. Biofeedback treatment of constlpation:A comparison of two methods[J]. Am J Gastroenterol,1994,89(7):1021-1026.
[8]	Xiong L,Gong X,Siah K T,et al. Rome foundation Asian working team report:real world treatment experience of Asian patients with functional bowel disorders[J]. J Gastroenterol Hepatol,2017,32(8):1450-1456. doi: 10.1111/jgh.13730
[9]	Ranasinghe N,Devanarayana N M,Benninga M A,et al. Psychological maladjustment and quality of life in adolescents with constipation[J]. Arch Dis Child,2017,102(3):268-273.
[10]	Di Lorenzo C,Flores A F,Reddy S N,et al. Use of colonic manometry to differentiate causes of intractable constipation in children[J]. J Pediatr,1992,120(5):690‐695.
[11]	刘海宁,陈玉琢,吴昊,等. 肠道菌群与功能性便秘的研究进展[J]. 复旦学报(医学版),2015,42(4):564-568. doi: 10.3969/j.issn.1672-8467.2015.04.024
[12]	毕洪玲,张桂兰,何嫱. 便秘患者肠菌群的调查[J]. 临床军医杂志,2003,31(3):82-84. doi: 10.3969/j.issn.1671-3826.2003.03.035
[13]	de Moraes J G,Motta M E,Beltrao M F,et al. Fecal microbiota and diet of children with chronic constipationn[J]. Int J Pediatr,2016,2016:6787269.
[14]	Gerritsen J,Smidt H,Rijkers G T. Intestinal microbiota in human health and disease:the impact of probiotics[J]. Genes Nutr,2011,6(3):209-240. doi: 10.1007/s12263-011-0229-7
[15]	Zoppi G,Cinquetti M,Luciano A,et a1. The intestinal ecosystem in chronic functional constipation[J]. Acta paediatrica,1998,87(8):836-841. doi: 10.1111/j.1651-2227.1998.tb01547.x
[16]	de Meij T G,de Groot E F,Eck A,et a1. Characterization of microbiota in children with chronic functional constipation[J]. PloS One,2016,11(10):e0164731. doi: 10.1371/journal.pone.0164731
[17]	朱俊晨,李世敏,魏小青. 双歧杆菌对人体的生理作用及研究进展[J]. 中国微生态学杂志,2002,12(1):53-55.
[18]	Desbonnet L,Garrett L,Clarke G,et a1. Effects of the probiotic Bifidobacterium infantis in the maternal separation model of depression[J]. Neuroscience,2010,170(4):1179-1188. doi: 10.1016/j.neuroscience.2010.08.005
[19]	Roma E,Adamidis D,Nikolara,et a1. Diet and chronic constipation in children:the role of fiber[J]. J Pediatr Gastroenterol Nutr,1999,28(1):169-174.
[20]	Suares N C,Ford A C. Systematic review:the effects of fibre in the management of chronic idiopathic constipation[J]. Aliment pharmacol Ther,2011,33(8):895-901. doi: 10.1111/j.1365-2036.2011.04602.x
[21]	Guerra P V,Lima L N,Souza T C,et al. Pediatric functional constipation treatment with Bifidobacterium-containing yogurt:a crossover,double-blind,controlled trial[J]. World J Gastroenterol,2011,17(34):3916-3921. doi: 10.3748/wjg.v17.i34.3916

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