Effects of Willed Movement on the Expression of GLUA2 and N-cadherin in Rats with Focal Cerebral Ischemia

Wen JIANG; Song MEI; Haijiang LI; Guoliang JIANG; Dan HUANG

doi:10.12259/j.issn.2095-610X.S20220320

Volume 43 Issue 3

Mar. 2022

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Wen JIANG, Song MEI, Haijiang LI, Guoliang JIANG, Dan HUANG. Effects of Willed Movement on the Expression of GLUA2 and N-cadherin in Rats with Focal Cerebral Ischemia[J]. Journal of Kunming Medical University, 2022, 43(3): 21-26. doi: 10.12259/j.issn.2095-610X.S20220320

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Wen JIANG, Song MEI, Haijiang LI, Guoliang JIANG, Dan HUANG. Effects of Willed Movement on the Expression of GLUA2 and N-cadherin in Rats with Focal Cerebral Ischemia[J]. Journal of Kunming Medical University, 2022, 43(3): 21-26. doi: 10.12259/j.issn.2095-610X.S20220320

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Effects of Willed Movement on the Expression of GLUA2 and N-cadherin in Rats with Focal Cerebral Ischemia

doi: 10.12259/j.issn.2095-610X.S20220320

1.
Dept. of Neurology，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032
2.
Dept. of Renal Endocrinology，Kunming Tongren Hospital， Kunming Yunnan 650228，China

Received Date: 2022-01-22
Available Online: 2022-02-18
Publish Date: 2022-03-22

Abstract

Abstract

Objective To explore effect of the Willed movement on behaviours of rats with focal cerebral ischemia and the expression of GluA2 and N-cadherin in the brain tissue around ischemia. Methods We selected clean-level healthy male SD rats, and prepared 144 middle cerebral artery occlusion （MCAO） models by thread occlusion method, and randomly divided them into model （MCAO） group, environment modification （EM） group, control rehabilitstion （CR） group and Willed movement （WM） group, each group was divided into four subgroups of 7, 15, and 30 days according to the postoperative time. The behavioral changes of rats in each group were dynamically observed, and the expression changes of GluA2 and N-cadherin protein in the brain tissue around the ischemic area were observed by immunohistochemical staining. Results On 7, 15, and 30 days after reperfusion, the climbing frequency of rats which were treated by willed movement was obviously higher than EM and CR group （P < 0.01）. After 30 days of reperfusion, the neurological deficits score of the intentional exercise intervention group was lower than MCAO rats （P < 0.05）. Compare with MCAO rats, the expression levels of GLUA2 and N-cadherin were higher in ischemic penumbra （P < 0.05）. Conclusions Willed movement can promote the recovery of damaged nerve function after focal cerebral ischemia, which may be related to up-regulating the expression of GLUA2 and N-cadherin in the brain tissue around the ischemic focus and enhancing synaptic plasticity.
- Focal cerebral ischemia,
- Willed movement,
- Synaptic plasticity,
- GLUA2,
- N-cadherin

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References

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