Characteristics of katG and inhA Gene Mutations in INH-Resistant Mycobacterium Tuberculosis in Yunnan Province

Lianyong CHEN; Haohao RU; Xing YANG; Shuangqun YAN; Tao CHEN; Qinxuan NI; Lin XU

doi:10.12259/j.issn.2095-610X.S20220803

Volume 43 Issue 8

Jul. 2022

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2022 > 43(8): 28-33

Lianyong CHEN, Haohao RU, Xing YANG, Shuangqun YAN, Tao CHEN, Qinxuan NI, Lin XU. Characteristics of katG and inhA Gene Mutations in INH-Resistant Mycobacterium Tuberculosis in Yunnan Province[J]. Journal of Kunming Medical University, 2022, 43(8): 28-33. doi: 10.12259/j.issn.2095-610X.S20220803

Citation:

Lianyong CHEN, Haohao RU, Xing YANG, Shuangqun YAN, Tao CHEN, Qinxuan NI, Lin XU. Characteristics of katG and inhA Gene Mutations in INH-Resistant Mycobacterium Tuberculosis in Yunnan Province[J]. Journal of Kunming Medical University, 2022, 43(8): 28-33. doi: 10.12259/j.issn.2095-610X.S20220803

Citation:

Lianyong CHEN, Haohao RU, Xing YANG, Shuangqun YAN, Tao CHEN, Qinxuan NI, Lin XU. Characteristics of katG and inhA Gene Mutations in INH-Resistant Mycobacterium Tuberculosis in Yunnan Province[J]. Journal of Kunming Medical University, 2022, 43(8): 28-33. doi: 10.12259/j.issn.2095-610X.S20220803

PDF( 999 KB)

Characteristics of katG and inhA Gene Mutations in INH-Resistant Mycobacterium Tuberculosis in Yunnan Province

doi: 10.12259/j.issn.2095-610X.S20220803

Dispensary for Tuberculosis Control and Prevention，Yunnan Center for Disease Control and Prevention，Kunming Yunnan 650022，China

Received Date: 2022-04-15
Available Online: 2022-07-19
Publish Date: 2022-07-28

Abstract

Abstract

Objective To analyze the characteristics of katG and inhA genetic mutations associated with INH-resistant Mycobacterium tuberculosis in Yunnan Province. Methods The INH-resistant Mycobacterium tuberculosis isolates were collected from drug-resistant monitoring sites in Yunnan Province, and the katG and inhA genes were amplified by PCR and then sequenced. Results Among the 88 MTB strains, the coincidence rate of INH-resistant genotype and phenotype was 82.95% （73/88）. 75.00% （66/88） had katG mutations and 9.09% （8/88） had inhA mutations. katG315 （67.05%） and inhA-15 （7.95%） were the top two mutation sites, and a high prevalence of katG Ser315Thr mutations （59.09%, 52/88） was observed. The frequency of the katG mutation was significantly higher in MDR-TB compared to INH mono-resistant isolates （χ² = 4.190, P = 0.041）, while katG315, inhA and inhA-15T mutations did not differ significantly between these two groups （P > 0.05）. Conclusion The most common mutation of INH-resistant Mycobacterium tuberculosis in Yunnan Province were katG315 and inhA-15, and katG mutation was higher among MDR isolates compared with INH mono-resistant strains. Molecular diagnostics based on gene mutations was a useful method for rapid detection of INH-resistant Mycobacterium tuberculosis in Yunnan Province.
- Mycobacterium tuberculosis,
- INH,
- Drug resistance,
- katG,
- inhA

FullText(HTML)

References(20)

References

[1]	World Health Organization. Global tuberculosis report2020[R]. Geneva, Switzerland: WHO, 2020.
[2]	李桂莲,万康林. 结核分枝杆菌异烟肼耐药性——一个不容忽视的问题[J]. 中国人兽共患病学报,2019,35(6):475-479.
[3]	杨慧娟,陈连勇,茹浩浩,等. 云南省结核分枝杆菌利福平耐药基因rpoB突变特征分析[J]. 中华疾病控制杂志,2018,22(2):147-152.
[4]	Tomasz Jagielski,Zofia Bakuła,Katarzyna Roeske,et al. Mutation profiling for detection of isoniazid resistance in Mycobacterium tuberculosis clinical isolates[J]. J Antimicrob Chemother,2015,70(12):3214-3221.
[5]	Molecular analysis of isoniazid resistance in different genotypes of Mycobacterium tuberculosis isolates from Iran[J]. Microbial Drug Resistance, 2008, 14（4）: 273-279.
[6]	Isakova J,Sovkhozova N,Vinnikov D,et al. Mutations of rpoB, katG, inhA and ahp genes in rifampicin and isoniazid-resistant Mycobacterium tuberculosis in Kyrgyz Republic[J]. BMC Microbiol,2018,18(1):1-8.
[7]	陈珊,刘厚明,单万水. 结核分枝杆菌耐药表型与耐药基因型相关性研究进展[J]. 中国感染控制杂志,2016,15(11):883-886.
[8]	Paolo Miotto,YingZhang,Daniela Maria,et al. Drug resistance mechanisms and drug susceptibility testing for tuberculosis[J]. Respirology,2018,23(12):1098-1113. doi: 10.1111/resp.13393
[9]	Zhao L L,Chen Y,Chen Z N,et al. Prevalence and molecular characteristics of drug-resistant Mycobacterium tuberculosis in Hunan,China[J]. Antimicrobial Agents and Chemotherapy,2014,58(6):3475-3480.
[10]	Thomas C Victor , Paul D van Helden,Robin Warren . Prediction of drug resistance in M. tuberculosis:Molecular mechanisms,tools,and applications[J]. IUBMB Life,2002,53(4):231-237.
[11]	Dong Luo,Qiang Chen,GuangchuXiong,et al. Prevalence and molecularcharacterization of multidrug-resistantM. tuberculosis in Jiangxiprovince,China[J]. Scientific Reports,2019,9(1):7315. doi: 10.1038/s41598-019-43547-2
[12]	张炜煜,杨修军,王慧,等. 吉林省耐多药结核分枝杆菌rpoB及katG基因突变特征分析[J]. 中国实验诊断学,2017,21(10):1731-1735.
[13]	赵刚,贾庆军,吴亦斐,等. 浙江地区耐多药结核分枝杆菌基因突变特征分析[J]. 中华疾病控制杂志,2021,25(1):66-71.
[14]	Jia Hongbing,Xu Yuhui,SunZhaogang. Analysis on drug-resistance-associated mutations among multidrug-resistant mycobacterium tuberculosis isolates in China[J]. Antibiotics,2021,10(11):1367-1377. doi: 10.3390/antibiotics10111367
[15]	文书,林璋礼,刘丁发,等. 耐异烟肼结核分枝杆菌及其katG、inhA基因的突变[J]. 中国热带医学,2019,19(8):723-726.
[16]	陈蕾. 四川地区结核分枝杆菌katG、inhA和rpoB基因突变位点耐药水平及突变频率特征分析[J]. 临床肺科杂志,2022,27(2):242-246.
[17]	王希江,谭云洪,贺文从,等. 结核分枝杆菌中利福平和异烟肼耐药相关基因突变与耐药水平的相关性研究[J]. 中国防痨杂志,2021,43(3):248-254.
[18]	Hu Y,Hoffner S,Jiang W,et al. Extensive transmission of isoniazid resistant M. tuberculosis and its association with increased multidrug-resistant TB in two rural counties of eastern China:A molecular epidemiological study[J]. BMC Infect Dis,2010,10(1):1-8. doi: 10.1186/1471-2334-10-43
[19]	Narmandakh E,Tumenbayar O,Borolzoi T,et al. Genetic mutations associated with isoniazid resistance in mycobacterium tuberculosis in Mongolia[J]. Antimicrob Agents Chemother,2020,64(7):1-7.
[20]	Chara,Asho,Sing,et al. Pattern of inhA and katG mutations in isoniazid monoresistant mycobacterium tuberculosis isolates[J]. Lung India,2020,37(3):227-231.

Relative Articles(20)

Relative Articles

[1]	Shi CHEN, Shi FU, Zhiyong TAN, Jiansong WANG, Haifeng WANG. Research Progress of Autophagy in the Development and Treatment of Bladder Cancer. Journal of Kunming Medical University, 2023, 44(5): 168-174. doi: 10.12259/j.issn.2095-610X.S20230503
[2]	Xiaomei JIN, Jieyan HE, Jichang SHI, Hui XING, Hong WANG, Ju ZHANG, Lijuan DONG, Lihua HUANG, Min CHEN, Zhijuan CHEN, Huichao CHEN. Virus Genotypes and Drug Resistance among HIV/AIDS Patients before Antiretroviral Therapy in Dali Bai Autonomous Prefecture in 2018. Journal of Kunming Medical University, 2023, 44(11): 152-157. doi: 10.12259/j.issn.2095-610X.S20231123
[3]	Shuangshuang WANG, Yaling LI, Liqin CHEN, Yonghui HAN, Mingbiao MA, Xiaojuan LI. The Analysis of Risk Factors and Drug Resistance of CRE Infection in Children’s Intensive Care Unit. Journal of Kunming Medical University, 2023, 44(9): 49-54. doi: 10.12259/j.issn.2095-610X.S20230918
[4]	Lianyong CHEN, Xing YANG, Haohao RU, Tao CHEN, Shuangqun YAN, Lin XU. Analysis of Molecular Characteristics of Mycobacterium Tuberculosis and Factors Associated with Transmission in Yunnan Province. Journal of Kunming Medical University, 2023, 44(8): 1-7. doi: 10.12259/j.issn.2095-610X.S20230821
[5]	Xinrui WAN, Xiaoyan WANG, Lin WANG, Zhiqiang MA, Caixin LI, Shitao XU, Xingrui TANG, Hongyan Mao. Liver，Kidney and Immunologic Function and Drug Resistance in Patients with Nontuberculous Mycobacteria Infection. Journal of Kunming Medical University, 2023, 44(11): 113-119. doi: 10.12259/j.issn.2095-610X.S20231117
[6]	Tingting YU, Hongying WANG, Runwu ZHANG, Jing BAI, Dong PU, Dongling LI. Analysis of Drug Resistance Mutation-related Sites in 1 452 Chronic Hepatitis B Patients with Low Viral Load in Kunming Area. Journal of Kunming Medical University, 2022, 43(9): 43-47. doi: 10.12259/j.issn.2095-610X.S20220927
[7]	Tingting YU, Dongling LI, Hongying WANG, Lihua LI, Dong PU, Kunli WU. Investigations on the Infection, Immunity and Drug Resistance of Newly-treated Cases of HIV in Kunming. Journal of Kunming Medical University, 2022, 43(1): 138-143. doi: 10.12259/j.issn.2095-610X.S20220133
[8]	Jinyi GU, Deyao DENG, Wenli YUAN, Qiuping YANG, Jianmei SONG, Yanjun SHEN, Yuanyuan GUO, Hongyun XU. Clinical Distribution and Drug Resistance Analysis of Acineto Bacterbaumannii from 2014 to 2020. Journal of Kunming Medical University, 2022, 43(5): 83-87. doi: 10.12259/j.issn.2095-610X.S20220504
[9]	Ling ZHOU, Feng-di HU, Rong LI, Ye-dan LIAO, Zheng-qin GENG, Jia-dai TANG, Xue-qi ZHANG, Lin XIE, Zuo-zhang YANG. A Clinical Scoring System Predicting the Osteosarcoma Chemotherapy Drug Resistance. Journal of Kunming Medical University, 2021, 42(1): 29-37. doi: 10.12259/j.issn.2095-610X.S20210144
[10]	Wen-jing SUN, Man-ting HU, Na LI, Fei GE, Wen-lin CHEN, Yang LIU. Progress in Clinical Research on Drug Resistance and Reversal of Endocrine Therapy in Breast Cancer. Journal of Kunming Medical University, 2021, 42(7): 143-149. doi: 10.12259/j.issn.2095-610X.S20210724
[11]	Yan-ling ZHANG, Hua HE, Ying HUANG, Yang LI, Jun-long WANG. Correlation Between Curative Effect and Concentration of Drugs in Cerebrospinal Fluid in Tuberculous Meningoencephalitis. Journal of Kunming Medical University, 2021, 42(12): 151-155. doi: 10.12259/j.issn.2095-610X.S20211235
[12]	Chen Lian Yong , Ru Hao Hao , Yang Xing , Yan Shuang Qun , Chen Jin Ou , Yang Rui , Chen Tao , Xu Lin . . Journal of Kunming Medical University, 2020, 41(03): 45-49.
[13]	Xiao-qin LI, Min ZHOU, Lin WANG, Hai-yan ZENG, Peng-ju LUO, Li-jia LIN. Efficacy of PCR-reverse Dot Hybridization in Drug Resistance Gene Detection and Drug Sensitivity of BD960 Tuberculosis in Drug Resistance Tuberculosis Detection. Journal of Kunming Medical University, 2020, 41(12): 138-141. doi: 10.12259/j.issn.2095-610X.S20201238
[14]	Li Ming Wu , Ma Meng , Zhang Rui Yu , Lai Ming Hong , Zhu Hui Qiong , Wan Rong . . Journal of Kunming Medical University, 2019, 40(01): 105-108.
[15]	Li Xiao Qin , Zhou Min , Wang Lin , Zeng Hai Yan , Luo Peng Ju , Li Ying , Ran Li Quan . Comparative Analysis of Rifampicin-resistant Mutant Gene Detection of Mycobacterium Tuberculosis and Drug Sensitivity BD960. Journal of Kunming Medical University, 2019, 40(03): 112-115.
[16]	Cheng Si Gen , Luo Zhi Gang , Liu De Hua , Yang Shu . 幽门螺杆菌清除治疗中甲硝唑、克拉霉素、阿莫西林的耐药性. Journal of Kunming Medical University, 2018, 39(04): 104-108.
[17]	Lian Yang Yang . . Journal of Kunming Medical University,
[18]	Dai Wei . . Journal of Kunming Medical University,
[19]	Chen Lian Yong . . Journal of Kunming Medical University,
[20]	. Resistance Analysis and Epidemiological Survey of 878 Acinetobacter Baumannii Solates. Journal of Kunming Medical University,