Determination of Scopolamine and Atropine in Blood and Urine by LC-MS / MS

Yuexin LIU; Fangdong TIAN; Xinyi LI; Gen XIONG; Xiangyu JIANG; Xian LI; Yanni LI

doi:10.12259/j.issn.2095-610X.S20220916

Volume 43 Issue 9

Sep. 2022

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Article Navigation > Journal of Kunming Medical University > 2022 > 43(9): 147-154

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Yuexin LIU, Fangdong TIAN, Xinyi LI, Gen XIONG, Xiangyu JIANG, Xian LI, Yanni LI. Determination of Scopolamine and Atropine in Blood and Urine by LC-MS / MS[J]. Journal of Kunming Medical University, 2022, 43(9): 147-154. doi: 10.12259/j.issn.2095-610X.S20220916

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Determination of Scopolamine and Atropine in Blood and Urine by LC-MS / MS

doi: 10.12259/j.issn.2095-610X.S20220916

1.
School of Forensic Medicine，Kunming Medical University，Kunming Yunnan 650500
2.
Yunnan Yingding Judicial Appraisal Center，Pu’er Yunnan 665000，China

Received Date: 2022-05-30
Available Online: 2022-09-07
Publish Date: 2022-09-29

Abstract

Abstract

Objective To develop a LC-MS/MS method for the determination of scopolamine and atropine in human blood and urine after Datura stramonium poisoning. Methods Shim-pack GISR-HP C18 column was used for gradient elution with acetonitrile formic acid （0.1%） as the mobile phase. The electrospray ionization ion source multiple reaction monitoring mode mass spectrometry was used to analyze the scopolamine and atropine by two characteristic ion pairs and the external standard curve method. Results The quantitative ion pairs of scopolamine and atropine were m/z 304.25 [M+H]+→138.15 and m/z 290.2 [M+H]+→124.15, respectively. The linear range of scopolamine and atropine were 1～100 ng/mL （R≥0.999）. The limits of detection （LOD） were 0.45 ng/ml and 0.19 ng/ml in blood, 0.38 ng/ml and 0.16 ng/ml in urine; The limits of quantitation （LOQ） were 1.01 ng/ml and 0.63 ng/ml in blood, 1.07 ng/ml and 0.38 ng/ml in urine; The extraction recoveries were 94.38% and 94.49% in blood, 94.96% and 96.52% in urine; The average recoveries of the substances were 94.38% and 94.49% in matrixs. The matrix effects effect was -3.51%～0.84%. The accuracy of substances were 89.38%～102.86%, and the intra day and inter day precisions were less than 6%. Conclusions The method can be used to determine the concentration of scopolamine and atropine in biological samples quickly and accurately.
- LC–MS/MS,
- Blood,
- Urine,
- Scopolamine,
- Atropine

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