Determination of Scopolamine and Atropine in Blood and Urine by LC-MS / MS

Yuexin LIU; Fangdong TIAN; Xinyi LI; Gen XIONG; Xiangyu JIANG; Xian LI; Yanni LI

doi:10.12259/j.issn.2095-610X.S20220916

Volume 43 Issue 9

Sep. 2022

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Yuexin LIU, Fangdong TIAN, Xinyi LI, Gen XIONG, Xiangyu JIANG, Xian LI, Yanni LI. Determination of Scopolamine and Atropine in Blood and Urine by LC-MS / MS[J]. Journal of Kunming Medical University, 2022, 43(9): 147-154. doi: 10.12259/j.issn.2095-610X.S20220916

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Determination of Scopolamine and Atropine in Blood and Urine by LC-MS / MS

doi: 10.12259/j.issn.2095-610X.S20220916

1.
School of Forensic Medicine，Kunming Medical University，Kunming Yunnan 650500
2.
Yunnan Yingding Judicial Appraisal Center，Pu’er Yunnan 665000，China

Received Date: 2022-05-30
Available Online: 2022-09-07
Publish Date: 2022-09-29

Abstract

Abstract

Objective To develop a LC-MS/MS method for the determination of scopolamine and atropine in human blood and urine after Datura stramonium poisoning. Methods Shim-pack GISR-HP C18 column was used for gradient elution with acetonitrile formic acid （0.1%） as the mobile phase. The electrospray ionization ion source multiple reaction monitoring mode mass spectrometry was used to analyze the scopolamine and atropine by two characteristic ion pairs and the external standard curve method. Results The quantitative ion pairs of scopolamine and atropine were m/z 304.25 [M+H]+→138.15 and m/z 290.2 [M+H]+→124.15, respectively. The linear range of scopolamine and atropine were 1～100 ng/mL （R≥0.999）. The limits of detection （LOD） were 0.45 ng/ml and 0.19 ng/ml in blood, 0.38 ng/ml and 0.16 ng/ml in urine; The limits of quantitation （LOQ） were 1.01 ng/ml and 0.63 ng/ml in blood, 1.07 ng/ml and 0.38 ng/ml in urine; The extraction recoveries were 94.38% and 94.49% in blood, 94.96% and 96.52% in urine; The average recoveries of the substances were 94.38% and 94.49% in matrixs. The matrix effects effect was -3.51%～0.84%. The accuracy of substances were 89.38%～102.86%, and the intra day and inter day precisions were less than 6%. Conclusions The method can be used to determine the concentration of scopolamine and atropine in biological samples quickly and accurately.
- LC–MS/MS,
- Blood,
- Urine,
- Scopolamine,
- Atropine

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References(11)

References

[1]	张宏利,杨学军,刘文国,等. 曼陀罗化学成分与生物活性研究现状及展望[J]. 西北林学院学报,2004,19(2):98-102. doi: 10.3969/j.issn.1001-7461.2004.02.030
[2]	Perharič L,Juvan K A,Stanovnik L. Acute effects of a low-dose atropine/ scopolamine mixture as a food contaminant inhuman volunteers[J]. Journal of Applied Toxicology,2013,33(9):980-990. doi: 10.1002/jat.2797
[3]	Zhang P,Li Y,Liu G,et al. Simultaneous determination of atropine,scopolamine,and anisodamine from Hyoscyamus niger L. in rat plasmaby high-performance liquid chromatography with tandem mass spectrometry and its application to a pharmacokinetics study[J]. Journal of Separation Science,2014,37(19):2664-2674. doi: 10.1002/jssc.201400534
[4]	Kintz P,Villain M,Barguil Y,et al. Testing for atropine and scopolamine in hair by LC-MS-MS after datura inoxia abuse[J]. Journal of Analytical Toxicology,2006,30(7):454-457. doi: 10.1093/jat/30.7.454
[5]	Tian F,Li C,Wang X,et al. Comparative study on pharmacokinetics of a series of anticholinergics,atropine,anisodamine,anisodine,scopolamine and tiotropium in rats[J]. Eur J Drug Metab Pharmacokinet,2015,40(3):245-253. doi: 10.1007/s13318-014-0192-y
[6]	Lakstygal A M,Kolesnikova T O,Khatsko S L,et al. DARK classics in chemical neuroscience:Atropine,scopolamine,and other anticholinergic deliriant hallucinogens[J]. ACS Chemical Neuroscience,2018,10(5):2144-2159.
[7]	Juliet N Olayinka,Anthony Eduviere,Olusegun Adeoluwa,et al. Quercetin mitigates scopolamine-induced memory dysfunction:Impact on oxidative stress and cholinergic mechanisms[J]. Metab Brain Dis,2022,37(1):265-277.
[8]	侯士果,谷学新,王书妍,等. HPLC测定洋金花中东莨菪碱和阿托品的含量[J]. 中国中药杂志,2006,31(13):1065-1067. doi: 10.3321/j.issn:1001-5302.2006.13.008
[9]	佘彩蒙,杜鸿雁,王芳琳,等. 超高效液相色谱-串联质谱法检测全血中的东莨菪碱和阿托品[J]. 刑事技术,2017,42(2):133-136. doi: 10.16467/j.1008-3650.2017.02.011
[10]	蒋运斌,苟琰,马逾英,等. 野生山莨菪根的生物碱类成分分析[J]. 时珍国医国药,2015,26(5):1243-1245.
[11]	任小娜,马永钧,周敏,等. 毛细管电泳-电致化学发光检测法分离测定中药马尿泡中的托烷类生物碱成分[J]. 色谱,2008,26(2):223-227. doi: 10.3321/j.issn:1000-8713.2008.02.016

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