Teng Yan . Effect of Hemoperfusion on Inflammation Factors in Patients with Sepsis[J]. Journal of Kunming Medical University, 2016, 37(05).
Citation: Yuexin LIU, Fangdong TIAN, Xinyi LI, Gen XIONG, Xiangyu JIANG, Xian LI, Yanni LI. Determination of Scopolamine and Atropine in Blood and Urine by LC-MS / MS[J]. Journal of Kunming Medical University, 2022, 43(9): 147-154. doi: 10.12259/j.issn.2095-610X.S20220916

Determination of Scopolamine and Atropine in Blood and Urine by LC-MS / MS

doi: 10.12259/j.issn.2095-610X.S20220916
  • Received Date: 2022-05-30
    Available Online: 2022-09-07
  • Publish Date: 2022-09-29
  •   Objective  To develop a LC-MS/MS method for the determination of scopolamine and atropine in human blood and urine after Datura stramonium poisoning.   Methods   Shim-pack GISR-HP C18 column was used for gradient elution with acetonitrile formic acid (0.1%) as the mobile phase. The electrospray ionization ion source multiple reaction monitoring mode mass spectrometry was used to analyze the scopolamine and atropine by two characteristic ion pairs and the external standard curve method.   Results  The quantitative ion pairs of scopolamine and atropine were m/z 304.25 [M+H]+→138.15 and m/z 290.2 [M+H]+→124.15, respectively. The linear range of scopolamine and atropine were 1~100 ng/mL (R≥0.999). The limits of detection (LOD) were 0.45 ng/ml and 0.19 ng/ml in blood, 0.38 ng/ml and 0.16 ng/ml in urine; The limits of quantitation (LOQ) were 1.01 ng/ml and 0.63 ng/ml in blood, 1.07 ng/ml and 0.38 ng/ml in urine; The extraction recoveries were 94.38% and 94.49% in blood, 94.96% and 96.52% in urine; The average recoveries of the substances were 94.38% and 94.49% in matrixs. The matrix effects effect was -3.51%~0.84%. The accuracy of substances were 89.38%~102.86%, and the intra day and inter day precisions were less than 6%.   Conclusions  The method can be used to determine the concentration of scopolamine and atropine in biological samples quickly and accurately.
  • [1]
    张宏利,杨学军,刘文国,等. 曼陀罗化学成分与生物活性研究现状及展望[J]. 西北林学院学报,2004,19(2):98-102. doi: 10.3969/j.issn.1001-7461.2004.02.030
    [2]
    Perharič L,Juvan K A,Stanovnik L. Acute effects of a low-dose atropine/ scopolamine mixture as a food contaminant inhuman volunteers[J]. Journal of Applied Toxicology,2013,33(9):980-990. doi: 10.1002/jat.2797
    [3]
    Zhang P,Li Y,Liu G,et al. Simultaneous determination of atropine,scopolamine,and anisodamine from Hyoscyamus niger L. in rat plasmaby high-performance liquid chromatography with tandem mass spectrometry and its application to a pharmacokinetics study[J]. Journal of Separation Science,2014,37(19):2664-2674. doi: 10.1002/jssc.201400534
    [4]
    Kintz P,Villain M,Barguil Y,et al. Testing for atropine and scopolamine in hair by LC-MS-MS after datura inoxia abuse[J]. Journal of Analytical Toxicology,2006,30(7):454-457. doi: 10.1093/jat/30.7.454
    [5]
    Tian F,Li C,Wang X,et al. Comparative study on pharmacokinetics of a series of anticholinergics,atropine,anisodamine,anisodine,scopolamine and tiotropium in rats[J]. Eur J Drug Metab Pharmacokinet,2015,40(3):245-253. doi: 10.1007/s13318-014-0192-y
    [6]
    Lakstygal A M,Kolesnikova T O,Khatsko S L,et al. DARK classics in chemical neuroscience:Atropine,scopolamine,and other anticholinergic deliriant hallucinogens[J]. ACS Chemical Neuroscience,2018,10(5):2144-2159.
    [7]
    Juliet N Olayinka,Anthony Eduviere,Olusegun Adeoluwa,et al. Quercetin mitigates scopolamine-induced memory dysfunction:Impact on oxidative stress and cholinergic mechanisms[J]. Metab Brain Dis,2022,37(1):265-277.
    [8]
    侯士果,谷学新,王书妍,等. HPLC测定洋金花中东莨菪碱和阿托品的含量[J]. 中国中药杂志,2006,31(13):1065-1067. doi: 10.3321/j.issn:1001-5302.2006.13.008
    [9]
    佘彩蒙,杜鸿雁,王芳琳,等. 超高效液相色谱-串联质谱法检测全血中的东莨菪碱和阿托品[J]. 刑事技术,2017,42(2):133-136. doi: 10.16467/j.1008-3650.2017.02.011
    [10]
    蒋运斌,苟琰,马逾英,等. 野生山莨菪根的生物碱类成分分析[J]. 时珍国医国药,2015,26(5):1243-1245.
    [11]
    任小娜,马永钧,周敏,等. 毛细管电泳-电致化学发光检测法分离测定中药马尿泡中的托烷类生物碱成分[J]. 色谱,2008,26(2):223-227. doi: 10.3321/j.issn:1000-8713.2008.02.016
  • Relative Articles

    [1] Fang LUO, Ruibin ZHAO, Guojun LI, Xuxian FU, Xianmou FAN, Shaoming SU, Yunbo WEN, Qilin HUANG, Bin LI. Researches on the Effect of Alcohol on Endogenous GHB in Human Blood by LC-MS/MS Method. Journal of Kunming Medical University, 2024, 45(5): 170-177.  doi: 10.12259/j.issn.2095-610X.S20240526
    [2] Canyu YANG, Kongchun SUN, Juan WANG, Huiping PU, Baochun SHEN. Metabolic Differences of Propranolol Enantiomers in Different Species of Liver Microsomes by HPLC-MS/MS. Journal of Kunming Medical University, 2023, 44(3): 15-21.  doi: 10.12259/j.issn.2095-610X.S20230321
    [3] Liping LI, Hongchao ZHAO, Huiqiong ZHAO, Jun LI. The Effect of Different Concentrations of Atropine on Myopia Prevention and Control in Adolescents. Journal of Kunming Medical University, 2023, 44(9): 80-85.  doi: 10.12259/j.issn.2095-610X.S20230903
    [4] Li-ping LI, Hong-chao ZHAO, Qian CHEN, Tao ZENG, Hui-qiong ZHAO, Wen-ji WANG, Na XU, Jun LI. Effect of Atropine Eye Drops Combined with Visual Function Training in Patients with Mild Myopia and Its Influence on Visual Acuity. Journal of Kunming Medical University, 2021, 42(6): 67-71.  doi: 10.12259/j.issn.2095-610X.S20210615
    [5] Xing-long CHEN, Min ZHANG, Qi-yao YANG, Yong-mou LUO, Ting-ting CAO, Li-ping TANG, Yong LI. Analysis of Triterpene Chemical Constituents in Poria cocos 95% Ethanol Extract via UPLC-IT-TOF/MS. Journal of Kunming Medical University, 2021, 42(11): 1-8.  doi: 10.12259/j.issn.2095-610X.S20211101
    [6] Guang-shun LIU, Xu-dong XIANG, Zhi-yong LV, Min ZHOU, Ya-hong WANG. The Application of Atropine in General Anaesthesia of Retaining Spontaneous Breathing for Painless Fiberoptic Bronchoscopy. Journal of Kunming Medical University, 2021, 42(9): 71-75.  doi: 10.12259/j.issn.2095-610X.S20210912
    [7] Yan-ming REN, Ji-bin XIONG, Chen-fei ZHAO, Chen-xi LI, An-qi GUAN, Zi-qing XU, Kai-bo ZHANG, Rui-lin ZHANG, Shu-hua LI. Effect of Acetone in Human Blood on Ethanol Detection Results. Journal of Kunming Medical University, 2021, 42(4): 146-149.  doi: 10.12259/j.issn.2095-610X.S20210428
    [8] Ming YAN, Jian-yun YU, Sheng-jie NIE, Yong-qiang QU, Shang-wen WANG, Rui WANG, Jun-jie SHU, Huan LIU, Shu-hua LI. Metabonomics of Scopolamine Intoxication in Rats. Journal of Kunming Medical University, 2021, 42(4): 13-19.  doi: 10.12259/j.issn.2095-610X.S20210403
    [9] Jiang En Yan , Yang Bo Wei , Liu Jian He . . Journal of Kunming Medical University, 2020, 41(08): 100-104.
    [10] Zhang Pei Pei , Cui Jia Li , Zhou Yi Jia , Liu Hong Bin , Ke Jin . . Journal of Kunming Medical University, 2020, 41(06): 39-45.
    [11] Gong Rong , Sun Wei , Xu Cong Qiong , Liu Lin , Wang Juan , Jin Zhen . . Journal of Kunming Medical University, 2019, 40(05): 73-79.
    [12] Lu Fa Huan , Li Ji Yin , Li Shu Hua , Zhao Wen Song , Wang Rui , Jie Run Fang , Yang Kai Run . Tissue Distribution of Yunaconitine in Rats by UPLC-MS/MS Method. Journal of Kunming Medical University, 2018, 39(05): 16-20.
    [13] Jie Run Fang , Tong Jun , Zhu Chen , Xu Tian Yong , Jie Ji Ming . Determination of Paraquat in Biomaterial by GC-MS with Water. Journal of Kunming Medical University, 2017, 38(07): 27-32.
    [14] Wang Shang Wen . Simultaneous Determination of Phenobarbital, Ibuprofen and Nikethamide in the Biomaterial with GC-MS. Journal of Kunming Medical University,
    [15] Dan Ke Ji . . Journal of Kunming Medical University,
    [16] Zhang Chun Xi . . Journal of Kunming Medical University,
    [17] Zhang Rong . . Journal of Kunming Medical University,
    [18] . . Journal of Kunming Medical University,
    [19] . Role of Continued Urine in the Formattion of Scar Tissue after Urethral Surgery. Journal of Kunming Medical University,
    [20] Liu Rui . . Journal of Kunming Medical University,
  • Cited by

    Periodical cited type(7)

    1. 孙宇,张晨雪,闫骥,武雪亮,张建锋,王立坤. 基因检测及超声灰度值预测结直肠癌肝转移患者消融治疗预后及影响因素分析. 河北医科大学学报. 2025(01): 99-107 .
    2. 杨晓娟,郭志武,马红丽,杨友锋,王浩,王奕江,李振勇. 人KRAS基因突变检测试剂盒的性能评价. 医疗装备. 2025(01): 27-31 .
    3. 杨楠,何乐,李振珺,刘凤磊,向奇,常守凤. 结直肠癌不同转移部位的KRAS、NRAS、BRAF、PIK3CA基因突变特点及其与预后的关系. 医学研究杂志. 2025(01): 48-53+115 .
    4. 张紫玉,刘畅,杨小英,周英,权伟,俸婷婷,刘雄伟. 苗药肺筋草调控NF-κB/JAK途径改善大鼠急性肺损伤的作用机制研究. 药物评价研究. 2024(11): 2548-2558 .
    5. 雷全国,王佳丽,黄小刚,谢增丁. 同期手术与分期手术治疗同时性结直肠癌肝转移的临床效果比较. 大医生. 2023(17): 66-69 .
    6. 谭晶,高波. KRAS、NRAS及BRAF基因突变状态对结直肠癌术后辅助化疗患者预后的影响. 河南医学研究. 2023(22): 4132-4135 .
    7. 杜劲,邱瑾,蔡文平,王文娟,庞小燕. KRAS、NRAS、BRAF、PIK3CA、NTRK联合检测与结直肠癌预后的相关性分析. 智慧健康. 2023(27): 113-116 .

    Other cited types(1)

  • Created with Highcharts 5.0.7Amount of accessChart context menuAbstract Views, HTML Views, PDF Downloads StatisticsAbstract ViewsHTML ViewsPDF Downloads2024-052024-062024-072024-082024-092024-102024-112024-122025-012025-022025-032025-040255075100125150
    Created with Highcharts 5.0.7Chart context menuAccess Class DistributionFULLTEXT: 48.6 %FULLTEXT: 48.6 %META: 50.7 %META: 50.7 %PDF: 0.7 %PDF: 0.7 %FULLTEXTMETAPDF
    Created with Highcharts 5.0.7Chart context menuAccess Area Distribution其他: 7.0 %其他: 7.0 %其他: 0.1 %其他: 0.1 %Falkenstein: 0.6 %Falkenstein: 0.6 %上海: 0.1 %上海: 0.1 %临沂: 0.1 %临沂: 0.1 %九江: 0.2 %九江: 0.2 %佛山: 0.1 %佛山: 0.1 %保定: 0.1 %保定: 0.1 %北京: 35.3 %北京: 35.3 %北海: 0.1 %北海: 0.1 %十堰: 0.2 %十堰: 0.2 %南京: 0.2 %南京: 0.2 %南宁: 0.3 %南宁: 0.3 %南通: 0.1 %南通: 0.1 %博阿努瓦: 0.2 %博阿努瓦: 0.2 %台州: 0.1 %台州: 0.1 %咸阳: 0.1 %咸阳: 0.1 %嘉兴: 0.6 %嘉兴: 0.6 %大庆: 0.1 %大庆: 0.1 %大理: 0.2 %大理: 0.2 %天津: 1.0 %天津: 1.0 %太原: 0.4 %太原: 0.4 %宣城: 0.2 %宣城: 0.2 %宿州: 0.1 %宿州: 0.1 %常州: 0.1 %常州: 0.1 %广州: 0.4 %广州: 0.4 %庆阳: 0.1 %庆阳: 0.1 %延安: 0.2 %延安: 0.2 %张家口: 2.4 %张家口: 2.4 %成都: 0.2 %成都: 0.2 %扬州: 1.0 %扬州: 1.0 %承德: 0.1 %承德: 0.1 %无锡: 0.1 %无锡: 0.1 %昆明: 1.4 %昆明: 1.4 %昭通: 0.5 %昭通: 0.5 %杭州: 0.8 %杭州: 0.8 %武汉: 0.3 %武汉: 0.3 %汕头: 0.1 %汕头: 0.1 %沈阳: 0.2 %沈阳: 0.2 %深圳: 0.5 %深圳: 0.5 %温州: 1.0 %温州: 1.0 %漯河: 3.8 %漯河: 3.8 %盐城: 0.2 %盐城: 0.2 %石家庄: 0.7 %石家庄: 0.7 %芒廷维尤: 12.7 %芒廷维尤: 12.7 %芝加哥: 2.6 %芝加哥: 2.6 %茂名: 0.2 %茂名: 0.2 %荆门: 0.2 %荆门: 0.2 %衡阳: 0.1 %衡阳: 0.1 %襄阳: 0.1 %襄阳: 0.1 %西宁: 7.9 %西宁: 7.9 %西安: 0.2 %西安: 0.2 %西雅图: 0.2 %西雅图: 0.2 %贵阳: 0.1 %贵阳: 0.1 %赣州: 0.1 %赣州: 0.1 %运城: 0.2 %运城: 0.2 %遵义: 0.1 %遵义: 0.1 %邯郸: 0.2 %邯郸: 0.2 %郑州: 0.5 %郑州: 0.5 %重庆: 0.4 %重庆: 0.4 %金华: 0.1 %金华: 0.1 %长沙: 1.1 %长沙: 1.1 %青岛: 0.2 %青岛: 0.2 %驻马店: 11.7 %驻马店: 11.7 %其他其他Falkenstein上海临沂九江佛山保定北京北海十堰南京南宁南通博阿努瓦台州咸阳嘉兴大庆大理天津太原宣城宿州常州广州庆阳延安张家口成都扬州承德无锡昆明昭通杭州武汉汕头沈阳深圳温州漯河盐城石家庄芒廷维尤芝加哥茂名荆门衡阳襄阳西宁西安西雅图贵阳赣州运城遵义邯郸郑州重庆金华长沙青岛驻马店

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(1)  / Tables(12)

    Article Metrics

    Article views (3198) PDF downloads(19) Cited by(8)
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return