Effects of Dose-response and Time-dependent of Endotoxin on Proliferation of Human Umbilical Cord Mesenchymal Stem Cells

Wei YUAN; Mingyao MENG; Xinxin LI; Jingjing XIONG; Meng LI; Jia CAO; Mei LIU; Zongliu HOU; Yongkun HUANG

doi:10.12259/j.issn.2095-610X.S20220928

Volume 43 Issue 9

Sep. 2022

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Wei YUAN, Mingyao MENG, Xinxin LI, Jingjing XIONG, Meng LI, Jia CAO, Mei LIU, Zongliu HOU, Yongkun HUANG. Effects of Dose-response and Time-dependent of Endotoxin on Proliferation of Human Umbilical Cord Mesenchymal Stem Cells[J]. Journal of Kunming Medical University, 2022, 43(9): 7-12. doi: 10.12259/j.issn.2095-610X.S20220928

Citation:

Wei YUAN, Mingyao MENG, Xinxin LI, Jingjing XIONG, Meng LI, Jia CAO, Mei LIU, Zongliu HOU, Yongkun HUANG. Effects of Dose-response and Time-dependent of Endotoxin on Proliferation of Human Umbilical Cord Mesenchymal Stem Cells[J]. Journal of Kunming Medical University, 2022, 43(9): 7-12. doi: 10.12259/j.issn.2095-610X.S20220928

Citation:

Wei YUAN, Mingyao MENG, Xinxin LI, Jingjing XIONG, Meng LI, Jia CAO, Mei LIU, Zongliu HOU, Yongkun HUANG. Effects of Dose-response and Time-dependent of Endotoxin on Proliferation of Human Umbilical Cord Mesenchymal Stem Cells[J]. Journal of Kunming Medical University, 2022, 43(9): 7-12. doi: 10.12259/j.issn.2095-610X.S20220928

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Effects of Dose-response and Time-dependent of Endotoxin on Proliferation of Human Umbilical Cord Mesenchymal Stem Cells

doi: 10.12259/j.issn.2095-610X.S20220928

Wei YUAN¹,
Mingyao MENG^{2, 3},
Xinxin LI¹,
Jingjing XIONG^{1, 4},
Meng LI^{1, 4},
Jia CAO^{1, 4},
Mei LIU¹,
Zongliu HOU^{2, 3
,
,},
Yongkun HUANG^{1, 4
,
,}

1.
Dept. of Pediatrics，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032
2.
Yunnan Provincial Key Laboratory of Tumor Immunotherapy，Kunming Yunnan 650051
3.
Yan’an Hospital Affiliated to Kunming Medical University，Kunming Yunnan 650051
4.
Yunnan Provincial Key Laboratory of Laboratory Medicine，Kunming Yunnan 650032，China

Received Date: 2022-06-23
Available Online: 2022-09-07
Publish Date: 2022-09-29

Abstract

Abstract

Objective To investigate the effects of dose-response and time-dependent of endotoxin on the proliferation of human umbilical cord mesenchymal stem cells（hUC-MSCs）. Methods The hUC-MSCs were cultured in stem cell culture medium containing different concentrations of endotoxin（0.098, 0.195, 0.390, 0.780, 1.5600, 3.12, 6.250, 12.500, 25.000 and 50.000 EU/mL）. CCK-8 proliferation assay was used to detect the cell proliferation at 24, 48 and 72 hours. The low concentration（0.098 EU/mL）, medium concentration（1.56 EU/mL）and high concentration（25 EU/mL） of LPS were determined to promote the proliferation of hUC-MSCs, and the action time was selected for 72 hours. The effect of LPS on the proliferation of hUC-MSCs was further determined by flow cytometry. Results After 24, 48 hours of the treatment, it was mainly manifested by inhibiting the proliferation of hUC-MSCs. After 72 hours of the treatment, it showed a trend of promoting cell proliferation, and the promotion effect was most significant in the low concentration area, and the promotion effect was relatively weakened when the concentration increased. Further flow cytometry results suggested that the proportion of G0/G1 phase decreased, S phase and G2/M phase increased in LPS group of low concentration. Conclusion The dose-response and time-dependent of endotoxin have different effects on the proliferation of hUC-MSCs , and the appropriate concentration and duration of action of endotoxin are beneficial to the proliferation of hUC-MSCs.
- Endotoxin,
- Human umbilical cord mesenchymal stem cells,
- Cell proliferation,
- Stem cell transplantation

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