Role and Mechanism of TGF β1 in Platelet Promoting Invasion and Migration of Anaplastic Thyroid Carcinoma Cells

Yu’e JIANG; Zhaoming ZHONG; Yanzhang GAO; Wei WANG; Ruimei SUN; Chuanzheng SUN

doi:10.12259/j.issn.2095-610X.S20221201

Volume 43 Issue 12

Dec. 2022

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Yu’e JIANG, Zhaoming ZHONG, Yanzhang GAO, Wei WANG, Ruimei SUN, Chuanzheng SUN. Role and Mechanism of TGF β1 in Platelet Promoting Invasion and Migration of Anaplastic Thyroid Carcinoma Cells[J]. Journal of Kunming Medical University, 2022, 43(12): 1-5. doi: 10.12259/j.issn.2095-610X.S20221201

Citation:

Yu’e JIANG, Zhaoming ZHONG, Yanzhang GAO, Wei WANG, Ruimei SUN, Chuanzheng SUN. Role and Mechanism of TGF β1 in Platelet Promoting Invasion and Migration of Anaplastic Thyroid Carcinoma Cells[J]. Journal of Kunming Medical University, 2022, 43(12): 1-5. doi: 10.12259/j.issn.2095-610X.S20221201

Citation:

Yu’e JIANG, Zhaoming ZHONG, Yanzhang GAO, Wei WANG, Ruimei SUN, Chuanzheng SUN. Role and Mechanism of TGF β1 in Platelet Promoting Invasion and Migration of Anaplastic Thyroid Carcinoma Cells[J]. Journal of Kunming Medical University, 2022, 43(12): 1-5. doi: 10.12259/j.issn.2095-610X.S20221201

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Role and Mechanism of TGF β1 in Platelet Promoting Invasion and Migration of Anaplastic Thyroid Carcinoma Cells

doi: 10.12259/j.issn.2095-610X.S20221201

1.
Clinical Laboratory
2.
Dept. of Head and Neck Surgery Section II，The 3rd Affiliated Hospital of Kunming Medical University Tumor Hospital of Yunnan Province，Kunming Yunnan 650118
3.
Dept. of Oncology，The 1st Affiliated Hospital of Kunming Medical University， Kunming Yunnan 650032，China

Received Date: 2021-08-19
Available Online: 2022-12-05
Publish Date: 2022-12-25

Abstract

Abstract

Objective This study aims to explore whether platelet （PLT） is involved in regulating the progression of anaplastic thyroid carcinoma （ATC） by secreting transforming growth factor β1 （TGFβ1）. Methods The levels of TGFβ1 protein and mRNA in the supernatant of ATC cells were detected by ELISA and Real-time PCR, respectively. Transwell, scratch wound test and Western Blot were used to analyze the effects of TGFβ1 on the invasive and migratory abilities of ATC cell lines and its mechanism. Results In the co-culture supernatant of PLT and ATC cells, the protein level of TGFβ1 was significantly up-regulated, but the mRNA level was unchanged. PLT or rhTGF-β1 significantly increased the number of ATC cells crossing the chamber （P < 0.001）, suggesting that PLT or rhTGF-β1 could significantly enhance the migration ability of ATC cells. Scratch wound assay also confirmed that rhTGF-β1 could significantly enhance the migration ability of ATC cells. Conclusion PLT can significantly promote the development of EMT in ATC cells by secreting TGFβ1, and then promote the invasion and migration of ATC cells, suggesting that TGFβ1 may be a potential target of ATC molecular targeted therapy.
- Anaplastic thyroid carcinoma,
- Platelet,
- Transforming growth factor β1,
- Invasion and migration

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References(15)

References

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