miR-582-5p Regulating Notch1 to Reduce Myocardial Ischemia-reperfusion Injury

Feng WANG; Wei YANG; Dianlun QIAN; Song MEI; Wenjie WANG; Kexiang FENG; Xiangfeng BAI

doi:10.12259/j.issn.2095-610X.S20221207

Volume 43 Issue 12

Dec. 2022

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Feng WANG, Wei YANG, Dianlun QIAN, Song MEI, Wenjie WANG, Kexiang FENG, Xiangfeng BAI. miR-582-5p Regulating Notch1 to Reduce Myocardial Ischemia-reperfusion Injury[J]. Journal of Kunming Medical University, 2022, 43(12): 12-17. doi: 10.12259/j.issn.2095-610X.S20221207

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Feng WANG, Wei YANG, Dianlun QIAN, Song MEI, Wenjie WANG, Kexiang FENG, Xiangfeng BAI. miR-582-5p Regulating Notch1 to Reduce Myocardial Ischemia-reperfusion Injury[J]. Journal of Kunming Medical University, 2022, 43(12): 12-17. doi: 10.12259/j.issn.2095-610X.S20221207

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miR-582-5p Regulating Notch1 to Reduce Myocardial Ischemia-reperfusion Injury

doi: 10.12259/j.issn.2095-610X.S20221207

1.
Dept. of Laboratory Animal Science，Kunming Medical University，Kunming Yunnan 650500
2.
Dept. of Anesthesiology
3.
Dept. of Cardiac Surgery，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

Received Date: 2022-06-28
Available Online: 2022-12-05
Publish Date: 2022-12-25

Abstract

Abstract

Objective To investigate the role of miR-582-5p in regulating Notch1 to protect myocardium, by detecting the expression changes of miR-582-5p and Notch1 after myocardial ischemia reperfusion and HR model of HL-1 cells in mice. Methods The mouse myocardial ischemia-reperfusion model and HL-1 cell OGD reoxygenation model were established. The expression of miR-582-5p and Notch1 in mouse IR and cell HR was detected by qPCR, and then the targeted binding sites of miR-582-5p and Notch1 were predicted by TargetScan. Inhibitor and mimics of miR-582-5p and Notch1 were transfected into cells, and the expression of miR-582-5p and Notch1 was detected again. Finally, CCK-8 assay was used to detect the cell viability of HL-1 cells transfected with inhibitor and mimics. Results After HR, the expression of miR-582-5p was significantly increased （P < 0.01） and the expression of Notch1 was significantly decreased in IR mouse and HL-1 cells （P < 0.001）. After transfection of inhibitor, the expression of miR-582-5p was significantly down-regulated （P < 0.001）, while Notch1 was significantly up-regulated （P = 0.017）, and the viability of cardiomyocytes was significantly increased （P = 0.006）, but the result was opposite after transfection of mimics. Conclusion miR-582-5p can protect myocardium against myocardial ischemia and hypoxia by regulating Notch1.
- Myocardial ischemia-reperfusion,
- Oxygen and glucose deprivation,
- miR-582-5p,
- Notch1

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References(20)

References

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