Intestinal Flora in Uricase-Deficient Rats

Dan LI; Xulian WAN; Lvyu LI; Yu YUN; Guangyun LUO; Weibing LIU; Gongfu LIN; Ning LI; Yongkun LI; Weigang DUAN

doi:10.12259/j.issn.2095-610X.S20230205

Volume 44 Issue 2

Feb. 2023

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Article Navigation > Journal of Kunming Medical University > 2023 > 44(2): 27-32

Dan LI, Xulian WAN, Lvyu LI, Yu YUN, Guangyun LUO, Weibing LIU, Gongfu LIN, Ning LI, Yongkun LI, Weigang DUAN. Intestinal Flora in Uricase-Deficient Rats[J]. Journal of Kunming Medical University, 2023, 44(2): 27-32. doi: 10.12259/j.issn.2095-610X.S20230205

Citation:

Dan LI, Xulian WAN, Lvyu LI, Yu YUN, Guangyun LUO, Weibing LIU, Gongfu LIN, Ning LI, Yongkun LI, Weigang DUAN. Intestinal Flora in Uricase-Deficient Rats[J]. Journal of Kunming Medical University, 2023, 44(2): 27-32. doi: 10.12259/j.issn.2095-610X.S20230205

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Intestinal Flora in Uricase-Deficient Rats

doi: 10.12259/j.issn.2095-610X.S20230205

1.
School of Basic Medicine，Kunming Medical University，Kunming Yunan 650500
2.
Yunnan University of Chinese Medicine，Kunming Yunnan 650500
3.
Kunming Municipal Hospital of Traditional Chinese Medicine，Kunming Yunnan 650500
4.
Yunnan First People’s Hospital，Kunming Yunnan 650100
5.
Guangnan County People’s Hospital， Wenshan Yunnan 663300，China

Received Date: 2022-12-09
Publish Date: 2023-02-25

Abstract

Abstract

Objective To investigate the intestinal flora in male uricase-deficient rats （Kunming-DY, KDY rats） compared with wild-type SD rats of the same sex and age. Methods 45-day-old male uricase-deficient rats were weighed, and their blood was collected to obtain serum after they were anesthetized with uratan. Their small intestine was divided into 18 segments, and the colon was divided into 2 segments （5 cm each）. The serum uric acid （SUA） was detected by phosphotungstic acid method. DNA in intestinal segments and fresh feces was extracted using DNA extraction kit. High-throughput sequencing was performed after amplification with specific 16S rDNA primers. Results At 45 days of age, the body mass of KDY rats decreased （P < 0.05）, but the SUA level increased significantly （ P < 0.05）. The intestinal microbiota diversity in KDY rats decreased （ P < 0.05）, but their fecal microbiota diversity did not change significantly （ P > 0.05）. The abundance of Lactobacillus in intestinal tract and feces of KDY rats was significantly increased （P < 0.05）. Conclusion Compared with wild-type SD rats, the intestinal microbiota diversity of KDY rats decreased, but the abundance of Lactobacillus in intestinal tract and feces increased significantly.
- Uricase deficiency,
- Kunming-DY rats,
- Hyperuricemia,
- Intestinal flora,
- Lactobacillus

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References(15)

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