Chemical Compounds Isolated from <i>Cremastra Appendiculata</i> and Their Bioactive Activity

Yupeng LI; Xinglong CHEN; Shengxing YUAN; Yajuan CHEN; Rongping ZHANG

doi:10.12259/j.issn.2095-610X.S20230209

Volume 44 Issue 2

Feb. 2023

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Yupeng LI, Xinglong CHEN, Shengxing YUAN, Yajuan CHEN, Rongping ZHANG. Chemical Compounds Isolated from Cremastra Appendiculata and Their Bioactive Activity[J]. Journal of Kunming Medical University, 2023, 44(2): 1-6. doi: 10.12259/j.issn.2095-610X.S20230209

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Yupeng LI, Xinglong CHEN, Shengxing YUAN, Yajuan CHEN, Rongping ZHANG. Chemical Compounds Isolated from Cremastra Appendiculata and Their Bioactive Activity[J]. Journal of Kunming Medical University, 2023, 44(2): 1-6. doi: 10.12259/j.issn.2095-610X.S20230209

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Chemical Compounds Isolated from Cremastra Appendiculata and Their Bioactive Activity

doi: 10.12259/j.issn.2095-610X.S20230209

1.
School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products，Kunming Medical University，Kunming Yunnan 650500
2.
School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicine Utilization，Yunnan University of Chinese Medicine y，Kunming Yunnan 650500
3.
Dept. of Ultrasound，the First People’s Hospital of Qujing City，Yunnan Province，Kunming Yunnan 655000
4.
School of Rehabilitation，Kunming Medical University， Kunming Yunnan 650500，China

Received Date: 2022-12-16
Available Online: 2023-01-31
Publish Date: 2023-02-25

Abstract

Abstract

Objective To study the chemical compounds from the medicinal plants of Cremastra appendiculata and find its antitumor bioactive compounds. Methods The compounds were extracted by alcohol （95%） and isolated by column chromatography on silica gel and Sephadex LH-20. Their structures were identified by spectroscopic analysis （¹H NMR, ¹³CNMR and EIMS）. The antitumor activity of the compounds was studied by MTT assay in vitro. Results Twelve compounds were obtained and identified as bisbenzopyran （1）, （22E）-ergosta-6, 22-dien-3β, 5α, 8α-triol （2）, 3β-hydroxycholesta-5-ene （3）, β-sistosterol （4）, pinoresinol （5）, 5, 4′-dihydroxy-7- （4-hydroxybenzoyl） -3′- methoxyflavone （6）, 4-methoxy-2, 3, 7- trihydroxyphenanthrene （7）, 2-hydroxy-4, 7-dimethoxyphenanthrene （8）, 4, 4′-dimethoxy-[1, 1′-biphenanthrene]- 2, 2′, 7, 7′-tetrol （9）, 4, 7, 4′, 9′-tetramethoxy-[1, 1′-biphenanthrene] 2, 2′, 7, 7′-tetrol （10）, Bavachinine （11）, 3-hydroxyphenpropionic acid- （2′-methoxy-4′- carboxy-phenol） ester （12）; . The results of anti-tumor activity test of compounds 7～12 showed that 9 and 10 showed good inhibitory activity on MCF-7/S cell line. Conclusions Compounds 2, 3, 5, 7, 10 and 12 were isolated from this plant for the first time. IC₅₀ of 9 and 10 on MCF-7/S cell lines were 2.16 and 5.09 respectively μmol/L.
- Cremastra appendiculata,
- Chemical constituents,
- Bioactive activity

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References(22)

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