Effect of Resveratrol on Metastasis of Nasopharyngeal Carcinoma and Its Related Mechanism

Huajie WU; Dezhen TU; Lei LI; Han QIAO; Guoping LI

doi:10.12259/j.issn.2095-610X.S20230220

Volume 44 Issue 2

Feb. 2023

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Huajie WU, Dezhen TU, Lei LI, Han QIAO, Guoping LI. Effect of Resveratrol on Metastasis of Nasopharyngeal Carcinoma and Its Related Mechanism[J]. Journal of Kunming Medical University, 2023, 44(2): 14-22. doi: 10.12259/j.issn.2095-610X.S20230220

Citation:

Huajie WU, Dezhen TU, Lei LI, Han QIAO, Guoping LI. Effect of Resveratrol on Metastasis of Nasopharyngeal Carcinoma and Its Related Mechanism[J]. Journal of Kunming Medical University, 2023, 44(2): 14-22. doi: 10.12259/j.issn.2095-610X.S20230220

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Effect of Resveratrol on Metastasis of Nasopharyngeal Carcinoma and Its Related Mechanism

doi: 10.12259/j.issn.2095-610X.S20230220

Huajie WU¹,
Dezhen TU²,
Lei LI¹,
Han QIAO¹,
Guoping LI^{1
,
,}

1.
Head and Neck Department，Tumor Hospital of Yunnan Province，Kunming Yunnan 650118
2.
Otolaryngology Department，Children’s Hospital of Fuzhou City，Fuzhou Fujian 350005，China

Received Date: 2022-12-07
Available Online: 2023-02-01
Publish Date: 2023-02-25

Abstract

Abstract

Objective To observe the effect of resveratrol on metastasis of nasopharyngeal carcinoma and explore its molecular mechanism. Methods （1）The inhibiting ability of resveratrol on nasopharyngeal carcinoma cells CNE2 was evaluated by CCK8 assay at different concentrations and times （24 h/40 μmol/L, P < 0.01; 24 h/80 μmol/L and above, P < 0.001; 36 h/20 μmol/L, P < 0.05; 36 h/40 μmol/L and above, P < 0.001; 48 h/10 μmol/L, P < 0.01, 48 h/20 μmol/L and above, P < 0.001）. （2） Resveratrol of different concentrations treated CNE2 cells for 24 hours: the migration and invasion ability of CNE2 cells were detected by Transwell experiment; Western Blot was used to explore the expression of E-cadherin, Vimentin, Hsp27, Ezrin and MMP; the nuclear translocation of NF-κB/p65 in CNE2 cells were detected by immunofluorescence assay increased the expression of E-cadherin （20, 40 μmol/L, P < 0.01; 80 μmol/L, P < 0.001）, inhibited the expression of MMP9 and MMP2 （MMP2, 20 μmol/L, P < 0.05; 40, 80 μmol/L, P < 0.001; MMP9, 10, 20 μmol/L, P < 0.05; 40, 80 μmol/L, P < 0.01）. （3）The LN metastasis experiment was constructed by injecting CNE2 cells into the left hind footpad of BALB/c nude mice. HE staining and immunohistochemistry staining was used to observe the effect of different doses of resveratrol gavage on the metastasis of nasopharyngeal carcinoma cells to popliteal lymph nodes Hsp27 and Ezrin （Hsp27, 20, 40 μmol/L, P < 0.05; 80 μmol/L, P < 0.01; Ezrin, 40 μmol/L, P < 0.05; 80 μmol/L, P < 0.01）. Results （1） CCK8 experiments showed that resveratrol had a toxic effect on nasopharyngeal carcinoma cell CNE2, and its toxicity was dose and time-dependent. （2） Transwell test found that resveratrol could inhibit the migration and invasion of nasopharyngeal carcinoma cell CNE2 in a dose and time-dependent manner. Western Blot assay showed that resveratrol significantly inhibited the expression of MMP9 and MMP2, and increased the expression of E-cadherin. Resveratrol inhibited the phosphorylation levels of Ezrin and Hsp27. Immunofluorescent experiments showed that resveratrol dose-dependently inhibited nuclear translocation of NF-κB/p65. （3） Animal experiments showed that resveratrol gavage treatment had no effect on the body weight of tumor-bearing nude mice, but significantly reduced the degree of swelling of the popliteal lymph nodes. Histological examination showed that resveratrol gavage significantly inhibited the metastasis of nasopharyngeal carcinoma cells from the subcutaneous tumor formation site to the popliteal lymph nodes in a dose-dependent manner. Conclusions Resveratrol can inhibit the metastasis of CNE2 cells in a time and dose-dependent manner. The mechanisms of resveratrol inhibiting the invasion and migration of CNE2 cells may relate to inhibiting the nuclear translocation of NF-κB/p65 by decreasing the phosphorylation value of Ezrin and Hsp27, and up-regulating the level of E-cadherin and down-regulating the expression of MMP2 and MMP9.
- Resveratrol,
- Nasopharyngeal carcinoma,
- Metastasis,
- Mechanism

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