The Regulation of VEGF Signal Axis by DATS and the Improvement of Formation of Placental Angiogenesis in URSA Mice

Jiamei SONG; Yang LIU; Tao SHI; Fangjie JIANG; Jingsi CHEN; Wen FAN; Yu LIANG; Yushi MENG

doi:10.12259/j.issn.2095-610X.S20230506

Volume 44 Issue 5

May 2023

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Jiamei SONG, Yang LIU, Tao SHI, Fangjie JIANG, Jingsi CHEN, Wen FAN, Yu LIANG, Yushi MENG. The Regulation of VEGF Signal Axis by DATS and the Improvement of Formation of Placental Angiogenesis in URSA Mice[J]. Journal of Kunming Medical University, 2023, 44(5): 6-11. doi: 10.12259/j.issn.2095-610X.S20230506

Citation:

Jiamei SONG, Yang LIU, Tao SHI, Fangjie JIANG, Jingsi CHEN, Wen FAN, Yu LIANG, Yushi MENG. The Regulation of VEGF Signal Axis by DATS and the Improvement of Formation of Placental Angiogenesis in URSA Mice[J]. Journal of Kunming Medical University, 2023, 44(5): 6-11. doi: 10.12259/j.issn.2095-610X.S20230506

Citation:

Jiamei SONG, Yang LIU, Tao SHI, Fangjie JIANG, Jingsi CHEN, Wen FAN, Yu LIANG, Yushi MENG. The Regulation of VEGF Signal Axis by DATS and the Improvement of Formation of Placental Angiogenesis in URSA Mice[J]. Journal of Kunming Medical University, 2023, 44(5): 6-11. doi: 10.12259/j.issn.2095-610X.S20230506

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The Regulation of VEGF Signal Axis by DATS and the Improvement of Formation of Placental Angiogenesis in URSA Mice

doi: 10.12259/j.issn.2095-610X.S20230506

1.
Dept. of Reproductive Medicine
2.
Dept. of Pain，The 2nd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101，China

Received Date: 2023-01-19
Available Online: 2023-05-11
Publish Date: 2023-05-25

Abstract

Abstract

Objective The aim of this study is to investigate the effect of Diallyl trisulfide （DATS） on placental angiogenesis in mice with unexplained recurrent spontaneous abortion （URSA） and to explore its mechanism based on the vascular endothelial growth factor （VEGF）/vascular endothelial growth factor receptor-2 （VEGFR-2） signaling pathway. Methods Sixteen URSA mice selected were randomly divided into the control group and the experimental group, with 8 mice in each group. The experimental group was given 98% pure DATS （200 μL suspension prepared according to 50 mg/（kg·d) by gavage. The rats in the control group were fed conventionally and given 200 μL PBS solution by gavage. After 18 days of continuous administration, Hydrogen sulfide （H₂S） content in the placental tissue was detected by spectrophotometer, and the relative expression of CD31, VEGFA and VEGFR2 mRNA in the placental tissue was detected by reverse transcriptase-polymerase chain reaction （RT-qPCR）. The protein expression levels of VEGFA and VEGFR2 in placenta were detected by Western blot. Results The spectrophotometer detection showed that the H₂S level in the experimental group （777.4922±72.9759） was significantly higher than that in the control group （529.1824±99.7489）（P < 0.05）; The RT-qPCR results showed that the relative expression of CD31 in the experimental group （0.0042±0.0006） was significantly higher than that in the control group （0.0020±0.0004）（P < 0.05）, the relative expression of VEGFA in the experimental group （0.7073±0.0677） was significantly higher than that in the control group （0.5200±0.0946）（P < 0.05）, and the relative expression of VEGFR2 in the experimental group （0.7304±0.1262） was significantly higher than that in the control group （0.3984±0.047）（P < 0.05）; Western blot results showed that DATS significantly increased the protein expression levels of VEGFA and VEGFR2 in placental tissue （P < 0.05）. Conclusion DATS can significantly increase the level of H₂S in URSA placental tissue and the relative expression levels of angiogenic factors CD31, VEGFA, and VEGFR2 in placental tissue. Through the VEGF signal axis, it can improve the formation of placental blood vessels in URSA mice. The relevant research results will provide scientific basis and reference value for the clinical application of DATS.
- DATS,
- VEGF signaling axis,
- URSA mice,
- Placental vessels

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