Volume 44 Issue 6
Jun.  2023
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Jianheng AO, Lei MIAO, Huiying WANG, Tianyun ZHANG, Tao WANG, Yonghang TANG, Liping HU, Shengjie NIE. Correlation between DNA Detection Rate and Sweat Pore Density of Palm Touch Biological Samples[J]. Journal of Kunming Medical University, 2023, 44(6): 136-142. doi: 10.12259/j.issn.2095-610X.S20230601
Citation: Jianheng AO, Lei MIAO, Huiying WANG, Tianyun ZHANG, Tao WANG, Yonghang TANG, Liping HU, Shengjie NIE. Correlation between DNA Detection Rate and Sweat Pore Density of Palm Touch Biological Samples[J]. Journal of Kunming Medical University, 2023, 44(6): 136-142. doi: 10.12259/j.issn.2095-610X.S20230601

Correlation between DNA Detection Rate and Sweat Pore Density of Palm Touch Biological Samples

doi: 10.12259/j.issn.2095-610X.S20230601
  • Received Date: 2023-03-09
    Available Online: 2023-06-16
  • Publish Date: 2023-06-25
  •   Objective  To study the relationship between the detection rate of touch DNA and sweat pore density in male palm touch biological samples, and explore the factors affecting the detection rate of touch DNA.   Methods  The right hand hypothenar palmprints of 73 male college students were collected by using the added layer fingerprinting method, and the sweat pore count was performed in specific site . Palm-touch biological samples of 35 individuals were collected by direct palm wiping method and indirect glass slide transfer wiping method. PCR was performed on the samples by direct amplification, and short tandem repeat (STR) results were analyzed. The correlation between STR allele detection rate and sweat pore density was analyzed.   Results  The success rate of comprehensive sweat pore collection was 84.93%, the density of the sweat pore was (531.41±97.69)/cm2, the minimum value was 309 /cm², and the maximum value was 814 /cm2. There was no correlation between the palm sweat pore density and the STR allele detection rates using direct and indirect wiping methods (P1 = 0.806, P2 = 0.701).   Conclusion   Two different experimental methods were used to test the DNA of the palm touch biological samples, both of which showed that there was no correlation between the sweat pore density and the detection rate of touch DNA, indicating that the sweat pore density was not the main or only influencing factor of the detection rate of touch DNA.
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