Effect of MiR-26a-5p Regulating SLC26A4 to Ameliorate Hearing Loss in Deafness

Linhai CONG; Yong TANG; Jiazhi YIN; Li DENG; Huifang GAO; Hongli FANG; Xin LI

doi:10.12259/j.issn.2095-610X.S20230603

Volume 44 Issue 6

Jun. 2023

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Article Navigation > Journal of Kunming Medical University > 2023 > 44(6): 14-18

Linhai CONG, Yong TANG, Jiazhi YIN, Li DENG, Huifang GAO, Hongli FANG, Xin LI. Effect of MiR-26a-5p Regulating SLC26A4 to Ameliorate Hearing Loss in Deafness[J]. Journal of Kunming Medical University, 2023, 44(6): 14-18. doi: 10.12259/j.issn.2095-610X.S20230603

Citation:

Linhai CONG, Yong TANG, Jiazhi YIN, Li DENG, Huifang GAO, Hongli FANG, Xin LI. Effect of MiR-26a-5p Regulating SLC26A4 to Ameliorate Hearing Loss in Deafness[J]. Journal of Kunming Medical University, 2023, 44(6): 14-18. doi: 10.12259/j.issn.2095-610X.S20230603

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Effect of MiR-26a-5p Regulating SLC26A4 to Ameliorate Hearing Loss in Deafness

doi: 10.12259/j.issn.2095-610X.S20230603

1.
Dept. of Otolaryngology
2.
Health Administration Center
3.
Clinical Laboratory，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

Received Date: 2023-03-11
Available Online: 2023-06-16
Publish Date: 2023-06-25

Abstract

Abstract

Objective To explore the effect of miR-26a-5p in regulating SLC26A4 to amiliorate hearing loss, and to detect the expression changes of miR-26a-5p and SLC26A4 in deaf mice and hearing loss mice. Methods A mouse hearing loss model and a mouse deafness model were constructed, and the auditory brainstem response test was used to detect the hearing in the right ear of mice to determine whether the models were successfully constructed. qPCR test was used to detect the relative expression of miR-26a-5p and SLC26A4 after hearing loss and deafness in mice were established. The specific site of miR-26a-5p targeting binding to SLC26A4 was predicted at TargetScan website. The interference and overexpression vector of miR-26a-5p and SLC26A4 were injected, and the expression changes of miR-26a-5p and SLC26A4 were detected. Results After hearing loss and deafness were established, the expression of miR-26a-5p was significantly decreased （P < 0.05）, while the expression of SLC26A4 was significantly increased （P < 0.05）. After antagomir injection, miR-26a-5p was significantly down-regulated （P = 0.047）, while SLC26A4 was significantly up-regulated （P = 0.014）; and the hearing threshold of mice decreased significantly （P < 0.05）, but the result was just the opposite after injection of miR-26a-5p agomir. Conclusion miR-26a-5p can ameliorate hearing loss through SLC26A4 and thus decelerate the development of deafness.
- Hearing loss,
- Deafness,
- miR-26a-5p,
- SLC26A4

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References(16)

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