Overexpression of ZIC1 Gene Inhibits Proliferation of Pleural Mesothelioma Cells by Activating P53 Signaling Pathway

Yongjun DENG; Qian CHEN; Jianbin ZOU; Zheng GONG; Huanpeng LIU

doi:10.12259/j.issn.2095-610X.S20240405

Volume 45 Issue 4

Apr. 2024

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2024 > 45(4): 35-40

Yongjun DENG, Qian CHEN, Jianbin ZOU, Zheng GONG, Huanpeng LIU. Overexpression of ZIC1 Gene Inhibits Proliferation of Pleural Mesothelioma Cells by Activating P53 Signaling Pathway[J]. Journal of Kunming Medical University, 2024, 45(4): 35-40. doi: 10.12259/j.issn.2095-610X.S20240405

Citation:

Yongjun DENG, Qian CHEN, Jianbin ZOU, Zheng GONG, Huanpeng LIU. Overexpression of ZIC1 Gene Inhibits Proliferation of Pleural Mesothelioma Cells by Activating P53 Signaling Pathway[J]. Journal of Kunming Medical University, 2024, 45(4): 35-40. doi: 10.12259/j.issn.2095-610X.S20240405

Citation:

Yongjun DENG, Qian CHEN, Jianbin ZOU, Zheng GONG, Huanpeng LIU. Overexpression of ZIC1 Gene Inhibits Proliferation of Pleural Mesothelioma Cells by Activating P53 Signaling Pathway[J]. Journal of Kunming Medical University, 2024, 45(4): 35-40. doi: 10.12259/j.issn.2095-610X.S20240405

PDF( 1159 KB)

Overexpression of ZIC1 Gene Inhibits Proliferation of Pleural Mesothelioma Cells by Activating P53 Signaling Pathway

doi: 10.12259/j.issn.2095-610X.S20240405

1.
Dept. of Thoracic Surgery，Affiliated Hospital of Yunnan University ，Kunming Yunnan 650021
2.
School of Public Health，Dali University，Dali Yunnan 671000，China

Received Date: 2023-12-21
Available Online: 2024-04-07
Publish Date: 2024-04-29

Abstract

Abstract

Objective To investigate the effect of cerebellar zinc finger structure 1 （ZIC1） gene overexpression on the proliferation and apoptosis of pleural mesothelioma cells （SMC-1 cells） and the corresponding molecular mechanism. Methods SMC-1 cells were transfected with lentivirus particles carrying ZIC1 gene as the experimental group, infected with empty lentivirus particles as the control group, and conventional cultured untransfected SMC-1 cells as the blank control group. Western blot was used to detect the expression of ZIC1 protein in the three groups. CCK-8 cell proliferation assay was used to detect the proliferation ability of each group, and Hoechst-PI double staining was used to detect the apoptosis of each group. The protein expression levels of P53, P21, MDM2 and P53 phosphorylation site Ser392 in P53-mediated apoptosis signaling pathway were detected by Western blot. Results Compared with the empty vector group and the control group, the expression of ZIC1 protein in the ZIC1 overexpression group was significantly increased, with a statistically significant difference （F = 4.665, P = 0.036）. Compared with the control group, the proliferation ability of SMC-1 cells in the ZIC1 overexpression group was significantly reduced, while the apoptosis of tumor cells was significantly increased, with a statistically significant difference （P < 0.05）. The main genes P53, P21, MDM2, and P53 Ser392 protein expression in the P53 signaling pathway in the ZIC1 gene overexpression group were significantly increased. Conclusion Overexpression of ZIC1 gene can inhibit the proliferation of pleural mesothelioma SMC-1 cells and promote their apoptosis. The possible mechanism is to activate the P53 gene mediated apoptosis signaling pathway by upregulating the expression of P53 gene phosphorylation sites.
- ZIC1,
- Pleural mesothelioma cells,
- P53 signal pathway,
- Proliferation,
- Apoptosis

FullText(HTML)

References(19)

References

[1]	Baas P,Schouwink H,Zoetmulder F A. Malignant pleural mesothelioma[J]. Ann Oncol,1998,9(2):139-149. doi: 10.1023/A:1008239116237
[2]	Saddoughi S A,Abdelsattar Z M,Blackmon S H. National trends in the epidemiology of malignant pleural mesothelioma: A national cancer data base study[J]. The Annals of Thoracic Surgery,2018,105(2):432-437. doi: 10.1016/j.athoracsur.2017.09.036
[3]	Perera N D,Mansfield A S. The evolving therapeutic landscape for malignant pleural mesothelioma[J]. Current Oncology Reports,2022,24(11):1413-1423. doi: 10.1007/s11912-022-01302-3
[4]	Han W,Cao F,Gao X J,et al. ZIC1 acts a tumor suppressor in breast cancer by targeting survivin[J]. International Journal of Oncology,2018,53(3):937-948.
[5]	Iyer A S,Shaik M R,Raufman J P,et al. The roles of zinc finger proteins in colorectal cancer[J]. International Journal of Molecular Sciences,2023,24(12):10249. doi: 10.3390/ijms241210249
[6]	Ge Q,Hu Y,He J,et al. Zic1 suppresses gastric cancer metastasis by regulating Wnt/β‐catenin signaling and epithelial‐mesenchymal transition[J]. The FASEB Journal,2020,34(2):2161-2172. doi: 10.1096/fj.201901372RR
[7]	Hou Y,Chen K,Liao R,et al. LINC01419-mediated epigenetic silencing of ZIC1 promotes metastasis in hepatocellular carcinoma through the PI3K/Akt signaling pathway[J]. Laboratory Investigation,2021,101(5):570-587. doi: 10.1038/s41374-021-00539-z
[8]	Thottappillil N,Gomez-Salazar MA,Xu M,et al. ZIC1 dictates osteogenesis versus adipogenesis in human mesenchymal progenitor cells via a Hedgehog dependent mechanism[J]. Stem Cells,2023,41(9):862-76. doi: 10.1093/stmcls/sxad047
[9]	Sun J,Hu L,Bok S,et al. A vertebral skeletal stem cell lineage driving metastasis[J]. Nature,2023,621(7979):602-609. doi: 10.1038/s41586-023-06519-1
[10]	莫世贤,邓勇军,刘焕鹏,等. Fibulin-3 表达对恶性胸膜间皮瘤细胞的影响[J]. 中国病理生理杂志,2019(3):448-453.
[11]	Scherpereel A,Wallyn F,Albelda S M,et al. Novel therapies for malignant pleural mesothelioma[J]. The Lancet Oncology,2018,19(3):e161-e72. doi: 10.1016/S1470-2045(18)30100-1
[12]	Bronte G,Incorvaia L,Rizzo S,et al. The resistance related to targeted therapy in malignant pleural mesothelioma: Why has not the target been hit yet?[J]. Critical Reviews in Oncology/Hematology,2016,107:20-32. doi: 10.1016/j.critrevonc.2016.08.011
[13]	Liu S,Liu X,Lin X,et al. Zinc finger proteins in the war on gastric cancer: Molecular mechanism and clinical potential[J]. Cells,2023,12(9):1314. doi: 10.3390/cells12091314
[14]	Ma G,Dai W,Sang A,et al. Roles of ZIC family genes in human gastric cancer[J]. International Journal of Molecular Medicine,2016,38(1):259-266. doi: 10.3892/ijmm.2016.2587
[15]	Hata AN,Rowley S,Archibald H L,et al. Synergistic activity and heterogeneous acquired resistance of combined MDM2 and MEK inhibition in KRAS mutant cancers[J]. Oncogene,2017,36(47):6581-6591. doi: 10.1038/onc.2017.258
[16]	Shen W,Tong D,Chen J,et al. Silencing oncogene cell division cycle associated 5 induces apoptosis and G1 phase arrest of non‐small cell lung cancer cells via P53‐p21 signaling pathway[J]. Journal of Clinical Laboratory Analysis,2022,36(5):e24396. doi: 10.1002/jcla.24396
[17]	Lucchesi C A,Zhang J,Ma B,et al. Disruption of the Rbm38-eIF4E complex with a synthetic peptide Pep8 increases P53 expression[J]. Cancer Research,2019,79(4):807-818. doi: 10.1158/0008-5472.CAN-18-2209
[18]	Ma Z,Liu X,Zhang Q,et al. Carvedilol suppresses malignant proliferation of mammary epithelial cells through inhibition of the ROS-mediated PI3K/AKT signaling pathway[J]. Oncology Reports,2019,41(2):811-818.
[19]	Nicolaou S T,Kannan S,Warwicker J,et al. Activation of P53: How phosphorylated Ser15 triggers sequential phosphorylation of P53 at Thr18 by CK1δ[J]. Proteins:Structure,Function,and Bioinformatics,2022,90(12):2009-2022. doi: 10.1002/prot.26393

Relative Articles(20)

Relative Articles

[1]	Xiaobing GUO, Xiaowen LI, Hengxi LI, Yan CAO, Ping LI. miR-212-3p Targeted Regulation of NAP1L1 Inhibits Glioma Cell Proliferation，Migration and EMT. Journal of Kunming Medical University, 2024, 45(11): 22-30. doi: 10.12259/j.issn.2095-610X.S20241104
[2]	Ting LI, Wei-Hua GUO. Research Progress of Glycolytic Reprogramming in Oral Squamous Cell Carcinoma. Journal of Kunming Medical University, 2023, 44(6): 155-161. doi: 10.12259/j.issn.2095-610X.S20230611
[3]	Hui WANG, Wenjun ZENG, Haiping ZHANG, Ruiwei GUO. Effects of Store-operated Calcium Channel Molecule Orai3 on Rat Coronary Artery Smooth Muscle Cells Proliferation. Journal of Kunming Medical University, 2023, 44(4): 16-22. doi: 10.12259/j.issn.2095-610X.S20230423
[4]	Caihong LIANG, Mingyao MENG, Xinxin LI, Jingjing XIONG, Meng LI, Mei LIU, Zongliu HOU, Yongkun HUANG. Effect of Intestinal Flora Metabolites Deoxycholic Acid on the Proliferation and Cell Cycle of Human Umbilical Cord Mesenchymal Stem Cells. Journal of Kunming Medical University, 2023, 44(4): 23-30. doi: 10.12259/j.issn.2095-610X.S20230402
[5]	Xiaohan WANG, Shanmao MOU, Cuifang HAO, Linlin REN, Min WANG, Tong ZHAO. Platelet-rich Plasma Promotes the Proliferation of Human Endometrial Mesenchymal Stem Cells (EnMSCs) through the PI3K/AKT/mTOR Signaling Pathway. Journal of Kunming Medical University, 2022, 43(1): 26-32. doi: 10.12259/j.issn.2095-610X.S20220126
[6]	Wei YUAN, Mingyao MENG, Xinxin LI, Jingjing XIONG, Meng LI, Jia CAO, Mei LIU, Zongliu HOU, Yongkun HUANG. Effects of Dose-response and Time-dependent of Endotoxin on Proliferation of Human Umbilical Cord Mesenchymal Stem Cells. Journal of Kunming Medical University, 2022, 43(9): 7-12. doi: 10.12259/j.issn.2095-610X.S20220928
[7]	Shibing QIAN, Mingyao MENG, Hongbin YU, Kaiwen DUAN, Changquan LI, Zhigang XIA. Effect of Panax Noto-ginseng Saponins on the Proliferation of Human Stem Cells from Apical Papilla. Journal of Kunming Medical University, 2022, 43(10): 22-27. doi: 10.12259/j.issn.2095-610X.S20221018
[8]	Jun-jie LI, Hai-yan JIANG, Wen-ya BAI, Si-ying HUO, Zhi-sheng SUN, Jian-lin SHAO. Effects of Silencing RND3 Expression on the Inflammatory Response and Apoptosis of Hippocampal Neurons Injured by Oxygen Glucose Deprivation/Reoxygenation. Journal of Kunming Medical University, 2021, 42(10): 14-21. doi: 10.12259/j.issn.2095-610X.S20211012
[9]	Shun-hui YIN, Jian-zhong ZHOU, Zi-liang LI. Tiger17 Promotes the Proliferation and Migration of Human Oral Mucosa Fibroblast Cells. Journal of Kunming Medical University, 2021, 42(8): 128-131. doi: 10.12259/j.issn.2095-610X.S20210823
[10]	Yu-hang QUAN, Zhong-hui WANG, Shan-shan LI, Guang-shun LIU. Sevoflurane Inhibits Xuanwei Lung Cancer Cell Line XWLC-05. Journal of Kunming Medical University, 2021, 42(12): 1-5. doi: 10.12259/j.issn.2095-610X.S20211202
[11]	Hu Zheng Xiong . Effect of TGF-β2 and geneX on the Proliferation and Osteogenic Differentiation of BrdU-labeled Bone Mesenchymal Stem Cells. Journal of Kunming Medical University,
[12]	Yuan Yong . . Journal of Kunming Medical University,
[13]	Pan Yan Li . . Journal of Kunming Medical University,
[14]	Zhang Jian . . Journal of Kunming Medical University,
[15]	Zhao Yu Min . . Journal of Kunming Medical University,
[16]	Sun Hai Mei . Effects of Ischemic Postconditioning on Myocardial Apoptosis in Non-infarct Zone in Pigs with Myocardium. Journal of Kunming Medical University,
[17]	Li Kun Lun . Effect of Survivin Gene Expression Inhibition by RNAi on Breast Cancer SKBr-3 Cells. Journal of Kunming Medical University,
[18]	. TRB3 Mediates Homocysteine-induced Inhibition of Endothelial Cells Proliferation. Journal of Kunming Medical University,
[19]	Zhao Xue Ying . . Journal of Kunming Medical University,
[20]	Li Guo Dong . . Journal of Kunming Medical University,