Histological and Ultrastructural Studies of Lung Tissues in A Piglet Model of Early Onset Scoliosis with Combination of the Thoracic Insufficiency Syndrome

Qitang LI; Xingfei DAI; Qingdong ZHANG; Rongshuang YAN; Cheng PAN; Derui FEI; Yujian MA; Shixin XU; Ying ZHANG

doi:10.12259/j.issn.2095-610X.S20240504

Volume 45 Issue 5

May 2024

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Qitang LI, Xingfei DAI, Qingdong ZHANG, Rongshuang YAN, Cheng PAN, Derui FEI, Yujian MA, Shixin XU, Ying ZHANG. Histological and Ultrastructural Studies of Lung Tissues in A Piglet Model of Early Onset Scoliosis with Combination of the Thoracic Insufficiency Syndrome[J]. Journal of Kunming Medical University, 2024, 45(5): 23-28. doi: 10.12259/j.issn.2095-610X.S20240504

Citation:

Qitang LI, Xingfei DAI, Qingdong ZHANG, Rongshuang YAN, Cheng PAN, Derui FEI, Yujian MA, Shixin XU, Ying ZHANG. Histological and Ultrastructural Studies of Lung Tissues in A Piglet Model of Early Onset Scoliosis with Combination of the Thoracic Insufficiency Syndrome[J]. Journal of Kunming Medical University, 2024, 45(5): 23-28. doi: 10.12259/j.issn.2095-610X.S20240504

Citation:

Qitang LI, Xingfei DAI, Qingdong ZHANG, Rongshuang YAN, Cheng PAN, Derui FEI, Yujian MA, Shixin XU, Ying ZHANG. Histological and Ultrastructural Studies of Lung Tissues in A Piglet Model of Early Onset Scoliosis with Combination of the Thoracic Insufficiency Syndrome[J]. Journal of Kunming Medical University, 2024, 45(5): 23-28. doi: 10.12259/j.issn.2095-610X.S20240504

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Histological and Ultrastructural Studies of Lung Tissues in A Piglet Model of Early Onset Scoliosis with Combination of the Thoracic Insufficiency Syndrome

doi: 10.12259/j.issn.2095-610X.S20240504

1.
Dept. of Orthopedics ，The 2nd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101
2.
Dept.of Orthopedics，Yuxi Hospital Affiliated to Kunming University of Science and Technology/The 2nd People’s Hospital of Yuxi ，Yuxi Yunnan 653100，China

Received Date: 2024-01-12
Available Online: 2024-04-29
Publish Date: 2024-05-31

Abstract

Abstract

Objective To explore the histological and ultrastructural changes in lung tissues of piglets with the early onset scoliosis （EOS） with the combination of thoracic insufficiency syndrome （TIS） before and after the treatment with the growth-friendly techniques. Methods Six-week-old piglets were selected and randomly divided into the control group （n=3）, the model group （n=5） and the treatment group （n=5）. An EOS+TIS piglet model was constructed at 6 weeks of age. At 14 weeks of age, orthodontic treatment was performed at the same time as the tethering was released. The piglets were euthanized at 18 weeks of age, and the lung tissues were taken for HE staining and Masson staining. Pathological observations were conducted on lung parenchyma and pulmonary vascular development in the three groups. Bronchopulmonary dysplasia and pulmonary fibrosis were evaluated quantitatively with the use of following indicators: radial alveolar count （RAC）, alveolar wall thickness （AWT）, alveolar septal density ratio （ASDR）, respiratory film thickness （RFT）, average collagen fiber volume （ACFV）, pulmonary capillary density （PCD）, pulmonary artery wall thickness ratio （WT%）, and pulmonary artery wall cross-sectional area ratio （WA%）. At the same time, the ultrastructural changes in the lung tissue of piglets model were observed under the transmission electron microscope. Results Compared with the control group, pathological observation of the three piglet groups showed that the model group exhibited the bronchopulmonary dysplasia and pulmonary fibrosis, while the treatment group showed the alleviation of these manifestations.The differences in these groups of young pigs RAC, AWT, ASDR, RFT, ACFV and PCD were statistically significant （P < 0.05）. The differences between the WT% and WA% groups were not statistically significant（P > 0.05）. Under the transmission electron microscopy, the cell structure of the model group was damaged and had the poor integrity, while the cell structure of the treatment group was improved. Conclusion The lung tissue of EOS+TIS piglets shows the characteristics of bronchopulmonary dysplasia and pulmonary fibrosis; using growth-friendly technology to treat EOS+TIS piglets, its bronchopulmonary dysplasia and pulmonary fibrosis have been improved to a certain extent.
- Early-onset scoliosis,
- Thoracic insufficiency syndrome,
- Bronchopulmonary dysplasia,
- Lung histopathology,
- Transmission electron microscopy

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