The Effect of Probiotics on the Expression of Aβ and the Protective Effect of Neurons in Rats with Cerebral Ischemia-Reperfusion Injury

Guojing LIANG; Kun JI; Kaichun ZHANG; Yufang ZHANG; Jing AN; Yuge ZHANG; Juan WEN; Haiyan REN

doi:10.12259/j.issn.2095-610X.S20240506

Volume 45 Issue 5

May 2024

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Guojing LIANG, Kun JI, Kaichun ZHANG, Yufang ZHANG, Jing AN, Yuge ZHANG, Juan WEN, Haiyan REN. The Effect of Probiotics on the Expression of Aβ and the Protective Effect of Neurons in Rats with Cerebral Ischemia-Reperfusion Injury[J]. Journal of Kunming Medical University, 2024, 45(5): 37-43. doi: 10.12259/j.issn.2095-610X.S20240506

Citation:

Guojing LIANG, Kun JI, Kaichun ZHANG, Yufang ZHANG, Jing AN, Yuge ZHANG, Juan WEN, Haiyan REN. The Effect of Probiotics on the Expression of Aβ and the Protective Effect of Neurons in Rats with Cerebral Ischemia-Reperfusion Injury[J]. Journal of Kunming Medical University, 2024, 45(5): 37-43. doi: 10.12259/j.issn.2095-610X.S20240506

Citation:

Guojing LIANG, Kun JI, Kaichun ZHANG, Yufang ZHANG, Jing AN, Yuge ZHANG, Juan WEN, Haiyan REN. The Effect of Probiotics on the Expression of Aβ and the Protective Effect of Neurons in Rats with Cerebral Ischemia-Reperfusion Injury[J]. Journal of Kunming Medical University, 2024, 45(5): 37-43. doi: 10.12259/j.issn.2095-610X.S20240506

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The Effect of Probiotics on the Expression of Aβ and the Protective Effect of Neurons in Rats with Cerebral Ischemia-Reperfusion Injury

doi: 10.12259/j.issn.2095-610X.S20240506

1.
The Basic Medical Department of Xinjiang Medical University，Urumqi Xinjiang 830011
2.
Dept. of Neurosurgery，The 6th Affiliated Hospital of Xinjiang Medical University，Urumqi Xinjiang 830011
3.
Dept. of Cardiovascular Medicine，Urumqi Friendship Hospital，Urumqi Xinjiang 830011
4.
PCR Biology Laboratory of Xinjiang Medical University，Nucleic Acid Detection Center of Xinjiang Uygur Autonomous Region; Urumqi Xinjiang 830011
5.
Dept. of Pathology ， Changge Municipal People’s Hospital，Changge Henan 461500
6.
Central Laboratory of laboratory and Equipment Management Division of Xinjiang Medical University，Urumqi Xinjiang 830011，China

Received Date: 2024-01-05
Available Online: 2024-04-30
Publish Date: 2024-05-31

Abstract

Abstract

Objective To explore the effect of probiotics interventionon of the expression of Aβ in rats with cerebral ischemia-reperfusion and the protective effect of neurons. Methods Male SD rats were selected as the experimental animals and randomly divided into Sham Group, Model Group, Probiotics Group and Edaravone Group. The common carotid artery was separated only to the Sham Group While a cerebral ischemia-reperfusion model was eatablished to the other groups. The degree of brain injury was evaluated using neurobehavioral scoring; The infarct area of rat brain tissue was observed using TTC staining; HE staining was used to observe the pathological and morphological changes of neurons in the CA1 and cortical regions of the hippocampus in rats; The expression of Aβ proteins was detected using immunohistochemistry experiments. Results Compared with the Sham Group, the Model Group showed an increase in neurobehavioral scores （P < 0.05）, an increase in cerebral infarction focus （P < 0.05）, neuronal damage in the hippocampal CA1 and cortical regions, and a significant upregulation of Aβ protein expression （P < 0.001, P < 0.05）; Compared with the Model Group, the Probiotics Group and Edaravone Group showed a decrease in neurobehavioral scores （P < 0.05） and a reduction in cerebral infarction focus （P < 0.05）. The Probiotic Group and the Edaravone Group showed the less neuronal damage in the hippocampal CA1 and cortical regions , and the Aβ protein expression levels were significantly down regulated in both groups （P < 0.001, P < 0.05）. The improvement was more significant in Edaravone Group. Conclusion Probiotics intervention can downregulate the expression of Aβ protein, alleviate the brain tissue damage and exert neuroprotective effects in rats with cerebral ischemia-reperfusion.
- Probiotics,
- Cerebral ischemia-reperfusion injury,
- β amyloid,
- Edaravone

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References(30)

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