The Effect of HIF-1α on Invasion and Metastasis of Gallbladder Cancer by Regulating the Epithelial-Mesenchymal Transformation

Peng LI; Renchao ZOU; Longao DAI; Songzhou FU; Dong WEI; Bo TANG

doi:10.12259/j.issn.2095-610X.S20240507

Volume 45 Issue 5

May 2024

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Peng LI, Renchao ZOU, Longao DAI, Songzhou FU, Dong WEI, Bo TANG. The Effect of HIF-1α on Invasion and Metastasis of Gallbladder Cancer by Regulating the Epithelial-Mesenchymal Transformation[J]. Journal of Kunming Medical University, 2024, 45(5): 44-48. doi: 10.12259/j.issn.2095-610X.S20240507

Citation:

Peng LI, Renchao ZOU, Longao DAI, Songzhou FU, Dong WEI, Bo TANG. The Effect of HIF-1α on Invasion and Metastasis of Gallbladder Cancer by Regulating the Epithelial-Mesenchymal Transformation[J]. Journal of Kunming Medical University, 2024, 45(5): 44-48. doi: 10.12259/j.issn.2095-610X.S20240507

Citation:

Peng LI, Renchao ZOU, Longao DAI, Songzhou FU, Dong WEI, Bo TANG. The Effect of HIF-1α on Invasion and Metastasis of Gallbladder Cancer by Regulating the Epithelial-Mesenchymal Transformation[J]. Journal of Kunming Medical University, 2024, 45(5): 44-48. doi: 10.12259/j.issn.2095-610X.S20240507

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The Effect of HIF-1α on Invasion and Metastasis of Gallbladder Cancer by Regulating the Epithelial-Mesenchymal Transformation

doi: 10.12259/j.issn.2095-610X.S20240507

Dept. of Hepatopancreatobiliary Surgery，The 2nd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

Received Date: 2023-12-25
Available Online: 2024-04-29
Publish Date: 2024-05-31

Abstract

Abstract

Objective To explore the effect of HIF-1α on the migration and invasion of gallbladder cancer cells and its related regulatory mechanism. Methods GBC-SD cells were constructed and transfected with lentivirus, and the experimental group （HIF-1α shRNA）, negative control group （empty lentivirus transfection） and blank control group （untreated） were obtained respectively. The expression levels of E-Cadherin, and N-Cadherin proteins were detected by Western blotting assay, Cell scratch assay was ued to detect the migratory ability of GBC-SD cells, and Transwell invasion assay was uesd to detect the invasive ability of GBC-SD cells. Results Compared with the control group, the expression level of E-Cadherin protein in the experimental group was significantly increased （P < 0.001）, while the expression level of N-Cadherin protein was significantly decreased （P < 0.001）, and there was no remarkable difference in the expression level of E-Cadherin and N-Cadherin proteins between the control groups （P both>0.05）. In the experimental group, the cell scratch healing degree was inferior to the control group （P < 0.001） , and the number of penetrating molds was also inferior to the control group （P < 0.001）, while there was no obvious otherness in the cell scratch healing degree and the number of penetrating molds between the control groups （P > 0.05）. Conclusion Targeted knockdown of HIF-1α can reduce the migration and invasion ability of gallbladder cancer cells, and its mechanism of action may be related to the regulation of epithelial mesenchymal transition.
- Gallbladder neoplasms,
- HIF-1α,
- Neoplasm metastasis,
- Neoplasm invasiveness,
- Epithelial-mesenchymal transition

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