Researches on the Effect of Alcohol on Endogenous GHB in Human Blood by LC-MS/MS Method

Fang LUO; Ruibin ZHAO; Guojun LI; Xuxian FU; Xianmou FAN; Shaoming SU; Yunbo WEN; Qilin HUANG; Bin LI

doi:10.12259/j.issn.2095-610X.S20240526

Volume 45 Issue 5

May 2024

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2024 > 45(5): 170-177

Fang LUO, Ruibin ZHAO, Guojun LI, Xuxian FU, Xianmou FAN, Shaoming SU, Yunbo WEN, Qilin HUANG, Bin LI. Researches on the Effect of Alcohol on Endogenous GHB in Human Blood by LC-MS/MS Method[J]. Journal of Kunming Medical University, 2024, 45(5): 170-177. doi: 10.12259/j.issn.2095-610X.S20240526

Citation:

Fang LUO, Ruibin ZHAO, Guojun LI, Xuxian FU, Xianmou FAN, Shaoming SU, Yunbo WEN, Qilin HUANG, Bin LI. Researches on the Effect of Alcohol on Endogenous GHB in Human Blood by LC-MS/MS Method[J]. Journal of Kunming Medical University, 2024, 45(5): 170-177. doi: 10.12259/j.issn.2095-610X.S20240526

Citation:

Fang LUO, Ruibin ZHAO, Guojun LI, Xuxian FU, Xianmou FAN, Shaoming SU, Yunbo WEN, Qilin HUANG, Bin LI. Researches on the Effect of Alcohol on Endogenous GHB in Human Blood by LC-MS/MS Method[J]. Journal of Kunming Medical University, 2024, 45(5): 170-177. doi: 10.12259/j.issn.2095-610X.S20240526

PDF( 1268 KB)

Researches on the Effect of Alcohol on Endogenous GHB in Human Blood by LC-MS/MS Method

doi: 10.12259/j.issn.2095-610X.S20240526

1.
Research Institution of Criminal Science and Technology of Kunming Public Security Bureau ，Kunming Yunnan 650500
2.
College of Forensic Medicine，Kunming Medical University，Kunming Yunnan 650500
3.
Yunnan Police College，Kunming Yunnan 650221，China

Received Date: 2024-01-12
Available Online: 2024-04-29
Publish Date: 2024-05-31

Abstract

Abstract

Objective To study the effect of alcohol on endogenous GHB content in human blood. Methods LC-MS/MS inspection method was established in the blood for γ- Hydroxybutyric acid （GHB）, γ- butyrolactone （GBL） and 1,4-butanediol （1,4-BD）. 22 healthy subjects were selected as the research subjects to simulate the real cases of drinking alcohol. The levels of GHB in their blood were detected before and after drinking to compare the endogenous GHB levels in the blood before and after drinking. Results GHB in the concentration range of 0.1～50 μg/mL had a good linear relationship and there was a good linear relationship between GBL and 1,4-BD in the concentration range of 0.15～50 μg/mL （R² > 0.999）.The limits of detection of GHB, GBL and 1,4-BD were 0.015 μg/mL, 0.025 μg/mL and 0.01 μg/mL, respectively, and the limits of GHB, GBL and 1,4-BD quantification were 0.1 μg/mL, 0.15 μg/mL and 0.15 μg/mL. The matrix effects of the three compounds ranged from 18.2% to 20.1%, the recoveries were 81.6% to 92.3%, the intra-day and inter-day precision of RSD were less than 10%, and the accuracy was -5.8%-7.0%. The content of GHB in the blood after 7 hours of drinking were higher than that before drinking alcohol (P < 0.05）, and the concentration range was 0.71-3.81 μg/mL. Conclusion The content of endogenous GHB in human blood has increased after 7 hours of drinking alcohol, but the concentration was lower than 4 μg/mL, which can provide the data support for the assessment of endogenous and exogenous GHB involved in real cases, enriching the forensic toxicology data of GHB .
- γ-hydroxybutyric acid,
- γ-Butyrolactone,
- 1,4-Butanediol,
- Alcohol,
- LC-MS/MS method

FullText(HTML)

References(17)

References

[1]	董慧茜,沈屹东,郝伟. 新型毒品γ-羟基丁酸中毒、戒断的研究[J]. 中国药物依赖性杂志,2013,22(6):401-407,411.
[2]	段炼,蓝晓嫦,杨欣湖. 使用“G水”所致精神和行为障碍一例[J]. 中国药物滥用防治杂志,2021,27(3):381-383,387.
[3]	Mannucci C,Pichini S,Spagnolo E V,et al. Sodium oxybate therapy for alcohol withdrawal syndrome and keeping of alcohol abstinence[J]. Current Drug Metabolism,2018,19(13):1056-1064. doi: 10.2174/1389200219666171207122227
[4]	Moriya F,Nishimura H,Furumiya J,et al. Effects of drinking and smoking on endogenous levels of urinary γ-hydroxybutyric acid,a preliminary study[J]. Legal Medicine,2006,8(4):231-234. doi: 10.1016/j.legalmed.2006.05.001
[5]	Poldrugo,F,Barker,S,Basa,M,et al. Ethanol potentiates the toxic effects of 1,4-butanediol[J]. Alcohol Clin Exp Res,1985,9(6):493-497. doi: 10.1111/j.1530-0277.1985.tb05589.x
[6]	Jung,S,Kim,M,Kim,S,et al. Interaction between γ-Hydroxybutyric acid and ethanol: A review from toxicokinetic and toxicodynamic perspectives[J]. Metabolites,2023,13(2):180. doi: 10.3390/metabo13020180
[7]	K ü ting T,Madea B,Hess C,et al. Comparative study: Postmortem long-term stability of endogenous GHB in cardiac blood,femoral blood,vitreous humor,cerebrospinal fluid and urine with and without sodium fluoride stabilization[J]. Journal of Analytical Toxicology,2022,46(5):519-527. doi: 10.1093/jat/bkab042
[8]	Korb A S,Cooper G. Endogenous concentrations of GHB in postmortem blood from deaths unrelated to GHB use[J]. Journal of Analytical Toxicology,2014,38(8):582-588. doi: 10.1093/jat/bku088
[9]	李润康,何洪源,李佳宜,等. 生物样品中γ-羟基丁酸的检验方法与阈值研究[J]. 分析测试学报,2021,40(4):488-494. doi: 10.3969/j.issn.1004-4957.2021.04.008
[10]	Elian A A. Determination of endogenous gamma-hydroxybutyric acid (GHB) levels in antemortem urine and blood[J]. Forensic Science International,2002,128(3):120-122. doi: 10.1016/S0379-0738(02)00183-4
[11]	中华人民共和国国家卫生健康委员会. 静脉血液标本采集指南[J]. 中国实用乡村医生杂志,2020,27(5):7-11. 中华人民共和国国家卫生健康委员会. 静脉血液标本采集指南[J]. 中国实用乡村医生杂志, 2020, 27（5）: 7-11.
[12]	Kietzerow J,Otto B,Wilke N,et al. The challenge of post-mortem GHB analysis: storage conditions and specimen types are both important[J]. International Journal of Legal Medicine,2020,134(1):205-215. doi: 10.1007/s00414-019-02150-w
[13]	Ha H H,Mata D C,Vargas J R. Endogenous gamma-hydroxybutyrate in postmortem samples[J]. J Anal Toxicol,2020,44(3):263-267. doi: 10.1093/jat/bkz094
[14]	Darke S,Peacock A,Duflou J,et al. Characteristics and circumstances of death related to gamma hydroxybutyrate (GHB)[J]. Clinical Toxicology,2020,58(11):1028-1033. doi: 10.1080/15563650.2020.1726378
[15]	Paolo F B ,Wayne A J . Interpreting γ-hydroxybutyrate concentrations for clinical and forensic purposes. [J]. Clinical Toxicology (Philadelphia,Pa. ),2019,57(3): 149-163.
[16]	Kugelberg F C,Holmgren A,Eklund A,et al. Forensic toxicology findings in deaths involving gamma-hydroxybutyrate[J]. International Journal of Legal Medicine,2010,122(1):1-6.
[17]	Caputo F,Vignoli T,Tarli C,et al. A brief up-date of the use of sodium oxybate for the treatment of alcohol use disorder[J]. Int J Environ Res Public Health,2016,13(3):290-290. doi: 10.3390/ijerph13030290

Relative Articles(20)

Relative Articles

[1]	Canyu YANG, Kongchun SUN, Juan WANG, Huiping PU, Baochun SHEN. Metabolic Differences of Propranolol Enantiomers in Different Species of Liver Microsomes by HPLC-MS/MS. Journal of Kunming Medical University, 2023, 44(3): 15-21. doi: 10.12259/j.issn.2095-610X.S20230321
[2]	Xu YOU, Jianping LIU, Baoshuang XUE, Huiyun YIN, Lifen ZHANG, Xiaoqin WU. Epidemiological Characteristics of Male Patients with the First-episode Alcohol-induced Psychiatric Disorders. Journal of Kunming Medical University, 2023, 44(5): 38-42. doi: 10.12259/j.issn.2095-610X.S20230525
[3]	Yuexin LIU, Fangdong TIAN, Xinyi LI, Gen XIONG, Xiangyu JIANG, Xian LI, Yanni LI. Determination of Scopolamine and Atropine in Blood and Urine by LC-MS / MS. Journal of Kunming Medical University, 2022, 43(9): 147-154. doi: 10.12259/j.issn.2095-610X.S20220916
[4]	Li-yuan AN, Bing LI, Gang SU. Effects of Different Doses of Ulinastatinon Postoperative Cognitive Function and HMGB1 in Elderly Patients. Journal of Kunming Medical University, 2021, 42(7): 121-125. doi: 10.12259/j.issn.2095-610X.S20210720
[5]	Chen Xi , Xu Zhi Xing , Liu Xu Jie , Tian Jin Tao , Niu Xiao Qun , Pu Jun . . Journal of Kunming Medical University, 2020, 41(09): 21-25.
[6]	Wang Bo , Xia Xian Song , Li Jin Tao , Duan Xiao Hua . . Journal of Kunming Medical University, 2020, 41(04): 11-17.
[7]	Yang Juan , Zhao Xiao Hui , Li Jin Shi , Liu Hui Qin , Wang Ling Ling , Ren Hai Bing , Wang Si Si , Yan Jing , Sui Hai Jing . . Journal of Kunming Medical University, 2019, 40(04): 101-106.
[8]	Lu Fa Huan , Li Ji Yin , Li Shu Hua , Zhao Wen Song , Wang Rui , Jie Run Fang , Yang Kai Run . Tissue Distribution of Yunaconitine in Rats by UPLC-MS/MS Method. Journal of Kunming Medical University, 2018, 39(05): 16-20.
[9]	Jie Run Fang , Tong Jun , Zhu Chen , Xu Tian Yong , Jie Ji Ming . Determination of Paraquat in Biomaterial by GC-MS with Water. Journal of Kunming Medical University, 2017, 38(07): 27-32.
[10]	Li Jin Tao , Ma Yi , Zhang Yu Wen , Tang Ying , Wang Qiong Xian , Zheng Hong . Effects of Alcohol on Hematological Indexes of Tupaia Belangeri Chinensis. Journal of Kunming Medical University, 2017, 38(07): 33-36.
[11]	Liu Rong . . Journal of Kunming Medical University,
[12]	Wang Zi Rui . . Journal of Kunming Medical University,
[13]	Li Peng Xiang . . Journal of Kunming Medical University,
[14]	Liu Bo . . Journal of Kunming Medical University,
[15]	Shen Li Xin . . Journal of Kunming Medical University,
[16]	Yu Ping . 沙丁胺醇在3，4 -二氯苯基异氰酸酯万古霉素手性柱上的对映体分离. Journal of Kunming Medical University,
[17]	Guo Wei Lin . . Journal of Kunming Medical University, 2012, 33(12): 1-2.
[18]	. . Journal of Kunming Medical University,
[19]	. Therapeutic Effect of Ulinastatin Combined with Xuebijing on Acute Pancreatitis Complicated with Acute Lung Injury. Journal of Kunming Medical University,
[20]	. Treatment Experiences of Ulinstatin on the Reperfusion Injury of Postoperative Artery of Lower Extremity Acute Ischemia. Journal of Kunming Medical University,