Effects of HSV-1 Mutant Strain M6 on Macrophage-Mediated Immune Response after Infecting Human Bronchial Epithelial Cells

Zhenxiao ZHANG; Jingjing ZHANG; Yun LIAO; Dandan LI; Heng LI; Longding LIU

doi:10.12259/j.issn.2095-610X.S20240702

Volume 45 Issue 7

Jul. 2024

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2024 > 45(7): 6-13

Zhenxiao ZHANG, Jingjing ZHANG, Yun LIAO, Dandan LI, Heng LI, Longding LIU. Effects of HSV-1 Mutant Strain M6 on Macrophage-Mediated Immune Response after Infecting Human Bronchial Epithelial Cells[J]. Journal of Kunming Medical University, 2024, 45(7): 6-13. doi: 10.12259/j.issn.2095-610X.S20240702

Citation:

Zhenxiao ZHANG, Jingjing ZHANG, Yun LIAO, Dandan LI, Heng LI, Longding LIU. Effects of HSV-1 Mutant Strain M6 on Macrophage-Mediated Immune Response after Infecting Human Bronchial Epithelial Cells[J]. Journal of Kunming Medical University, 2024, 45(7): 6-13. doi: 10.12259/j.issn.2095-610X.S20240702

Citation:

Zhenxiao ZHANG, Jingjing ZHANG, Yun LIAO, Dandan LI, Heng LI, Longding LIU. Effects of HSV-1 Mutant Strain M6 on Macrophage-Mediated Immune Response after Infecting Human Bronchial Epithelial Cells[J]. Journal of Kunming Medical University, 2024, 45(7): 6-13. doi: 10.12259/j.issn.2095-610X.S20240702

PDF( 1959 KB)

Effects of HSV-1 Mutant Strain M6 on Macrophage-Mediated Immune Response after Infecting Human Bronchial Epithelial Cells

doi: 10.12259/j.issn.2095-610X.S20240702

Zhenxiao ZHANG^{1, 2},
Jingjing ZHANG¹,
Yun LIAO^{1, 2},
Dandan LI^{1, 2},
Heng LI^{1, 2
,
,},
Longding LIU^{1, 2
,
,}

1.
Institute of Medical Biology，Chinese Academy of Medical Sciences & Peking Union Medical College，Kunming Yunnan 650118
2.
Key Laboratory of Systemic Innovative Research on Virus Vaccine，Chinese Academy of Medical Sciences，Kunming Yunnan 650118，China

Received Date: 2024-03-27
Available Online: 2024-06-14
Publish Date: 2024-07-25

Abstract

Abstract

Objective To explore the impact of herpes simplex virus type 1 （HSV-1） mutant strain M6 on macrophage-mediated immune responses after infecting human bronchial epithelial cells （16HBE cells）. Methods HSV-1 infection of 16HBE cells was conducted to assess cytokine alterations in the culture medium; Macrophages were co-cultured with supernatants from HSV-1-infected 16HBE cells and subsequently reintroduced into mice via tail vein infusion. Cytokine expression levels in mouse lymph nodes, changes in spleen T cell proportions, mouse neutralizing antibody levels, and specific T cell responses were measured on days 1, 3, 7, 28, 56, and 90. Results Following infection of 16HBE cells with the HSV-1 mutant strain, cytokine expression in the supernatant, associated with macrophage recruitment and activation, was elevated, albeit slightly lower than that observed in the wild-type strain group. Following the tail vein infusion experiment, the mutant strain group exhibited temporal changes in the proportions of inflammatory factors, chemokines, and T cells within the mouse lymph nodes, resulting in weaker humoral immunity compared to the wild-type strain group, yet eliciting stronger specific T cell responses, with only a few parameters showing significant differences from the wild-type strain group （P < 0.05）. Conclusion 16HBE cells infected with the HSV-1 mutant strain M6 can release cytokines that recruit and activate macrophages, allowing macrophages to carry specific activation information of the HSV-1 mutant strain, activating the host's immune system and inducing the host's Humoral immunity and cellular immunity.
- Herpes simplex virus 1,
- Human bronchial epithelial cells,
- Macrophages,
- Mutant strain

FullText(HTML)

References(20)

References

[1]	Kwon H,Bai Q,Baek H-J,et al. Soluble V domain of Nectin-1/HveC enables entry of herpes simplex virus type 1 (HSV-1) into HSV-resistant cells by binding to viral glycoprotein D[J]. Journal of Virology,2006,80(1):138-148. doi: 10.1128/JVI.80.1.138-148.2006
[2]	Johnston C,Magaret A,Son H,et al. Viral shedding 1 year following first-episode genital HSV-1 infection[J]. Jama,2022,328(17):1730-1739. doi: 10.1001/jama.2022.19061
[3]	Sharma D,Sharma S,Akojwar N,et al. An insight into current treatment strategies,their limitations,and ongoing developments in vaccine technologies against herpes simplex infections[J]. Vaccines (Basel),2023,11(2):206. doi: 10.3390/vaccines11020206
[4]	Ravindra N G,Alfajaro M M,Gasque V,et al. Single-cell longitudinal analysis of SARS-cov-2 infection in human airway epithelium identifies target cells,alterations in gene expression,and cell state changes[J]. PLoS Biol,2021,19(3):e3001143. doi: 10.1371/journal.pbio.3001143
[5]	Kim S R. Viral infection and airway epithelial immunity in asthma[J]. Int J Mol Sci,2022,23(17):9914. doi: 10.3390/ijms23179914
[6]	Kolli D,Velayutham T S,Casola A. Host-viral interactions: Role of pattern recognition receptors (PRRs) in human pneumovirus infections[J]. Pathogens,2013,2(2):232-263. doi: 10.3390/pathogens2020232
[7]	Liu C,Zhang X,Xiang Y,et al. Role of epithelial chemokines in the pathogenesis of airway inflammation in asthma[J]. Mol Med Rep,2018,17(5):6935-6941.
[8]	Yu S,Ge H,Li S,et al. Modulation of macrophage polarization by viruses: Turning off/on host antiviral responses[J]. Front Microbiol,2022,13:839585. doi: 10.3389/fmicb.2022.839585
[9]	Carty S A. Immunology 101: Fundamental immunology for the practicing hematologist[J]. Hematology Am Soc Hematol Educ Program,2021,2021(1):281-286. doi: 10.1182/hematology.2021000260
[10]	Hirose S,Wang S,Jaggi U,et al. IL-17A expression by both T cells and non-T cells contribute to HSV-IL-2-induced CNS demyelination[J]. Front Immunol,2023,14:1102486. doi: 10.3389/fimmu.2023.1102486
[11]	Jaggi U,Wang S,Tormanen K,et al. Role of herpes simplex virus type 1 (hsv-1) glycoprotein k (gk) pathogenic cd8(+) t cells in exacerbation of eye disease[J]. Front Immunol,2018,9:2895. doi: 10.3389/fimmu.2018.02895
[12]	Jaggi U,Matundan H H,Yu J,et al. Essential role of m1 macrophages in blocking cytokine storm and pathology associated with murine hsv-1 infection[J]. PLoS Pathog,2021,17(10):e1009999. doi: 10.1371/journal.ppat.1009999
[13]	Mott K,Brick D J,van Rooijen N,et al. Macrophages are important determinants of acute ocular hsv-1 infection in immunized mice[J]. Invest Ophthalmol Vis Sci,2007,48(12):5605-5615. doi: 10.1167/iovs.07-0894
[14]	Zhang J,Xu X,Duan S,et al. Characterization of the immunologic phenotype of dendritic cells infected with herpes simplex virus 1[J]. Front Immunol,2022,13:931740. doi: 10.3389/fimmu.2022.931740
[15]	Verzosa A L,McGeever L A,Bhark S J,et al. Herpes simplex virus 1 infection of neuronal and non-neuronal cells elicits specific innate immune responses and immune evasion mechanisms[J]. Front Immunol,2021,12:644664. doi: 10.3389/fimmu.2021.644664
[16]	Xu X,Feng X,Wang L,et al. A HSV1 mutant leads to an attenuated phenotype and induces immunity with a protective effect[J]. PLoS Pathog,2020,16(8):e1008703. doi: 10.1371/journal.ppat.1008703
[17]	La Rosa F,Agostini S,Bianchi A,et al. Herpes simplex virus-1 (HSV-1) infection induces a potent but ineffective ifn-lambda production in immune cells of ad and pd patients[J]. J Transl Med,2019,17(1):286. doi: 10.1186/s12967-019-2034-9
[18]	He T,Wang M,Cheng A,et al. Host shutoff activity of vhs and sox-like proteins: Role in viral survival and immune evasion[J]. Virol J,2020,17(1):1-11. doi: 10.1186/s12985-020-01336-8
[19]	Zhao J,Qin C,Liu Y,et al. Herpes simplex virus and pattern recognition receptors: An arms race[J]. Front Immunol,2020,11:613799.
[20]	Lee D H,Ghiasi H. Roles of m1 and m2 macrophages in herpes simplex virus 1 infectivity[J]. J Virol,2017,91(15):e00578-17.

Relative Articles(20)

Relative Articles

[1]	Lanbo FU, Chaolie RUAN, Changxu CHEN, Yu GAO, Xiaolin BAO, Bochuan LIU, Jian ZHOU, Weidong LI. Domestication and Biological Characteristics of a Vero Cell Line in Suspension Culture. Journal of Kunming Medical University, 2024, 45(6): 7-14. doi: 10.12259/j.issn.2095-610X.S20240602
[2]	Liang ZHANG, Baoquan WANG, Xifeng LEI, Xu WANG, Yang KE, Wei ZHANG. M2 Macrophage-derived Exosome miR-1246 Regulates the Growth and Invasion of Gastric Cancer Cells. Journal of Kunming Medical University, 2023, 44(7): 69-77. doi: 10.12259/j.issn.2095-610X.S20230724
[3]	Yanlong LIU, Xuefeng GUANG, Xiaolong YIN, Hailong DAI. Effects of miR-125a-3p on Atherosclerotic Plaque, M1/M2 Macrophages, MMP-9 and VEGF. Journal of Kunming Medical University, 2022, 43(9): 1-6. doi: 10.12259/j.issn.2095-610X.S20220915
[4]	Weijia ZHAO, Hongbin LI, Zonghan CHEN. Effects of Micafungin on the Activity of Candida Smooth in Macrophages. Journal of Kunming Medical University, 2022, 43(1): 14-19. doi: 10.12259/j.issn.2095-610X.S20220142
[5]	Xiao CAI, Xue-tao YI, Jing-qing YAO, Xin-yu DAI, Zhong-quan TANG, Ting OU, Xiao-min ZHAO, Yun-tao LI. The Regulation of Exosome Derived from Human Bone Marrow Mesenchymal Stem Cells on the Polarization of Glioma-associated Macrophages. Journal of Kunming Medical University, 2021, 42(1): 38-45. doi: 10.12259/j.issn.2095-610X.S20210101
[6]	Wang Juan , Su Guo Miao , Pan Guo Qing , Bian Li , Yang Zhe , Zeng Ding Tao . . Journal of Kunming Medical University, 2020, 41(09): 1-6.
[7]	Li Jing , Lei Wen , Deng Shu Hao , Fan Du , Pu Ling , Yang Dong Mei , Zhao Xiao Yuan , Li Yang , Dong Zhao Xing . . Journal of Kunming Medical University, 2019, 40(01): 10-13.
[8]	Yang Can , Gong Yu Hang , Wang Hai Feng , Li Hai Hao , Liu Jing Yu , Wang Wei , Wang Jian Song , Zuo Yi Gang , Chen Jian , Zhan Hui , Ding Ming Xia . The Research of Monoclonal Antibody KMP1 Inhibited Bladder Cancer EJ Cell Lines Growth and Metastasis in Vivo. Journal of Kunming Medical University, 2018, 39(05): 1-6.
[9]	Long Rui Qing , Liu Zhuo Hui , Zhang Fan , Li Shu Ling , Yu Yong Mei , Ruan Biao . Infection of Pseudomonas aeruginosa Activates TGF-β1 Smad Signaling Pathway in Human Middle Ear Epithelial Cells. Journal of Kunming Medical University, 2017, 38(10): 65-69.
[10]	Zhang Xiao Chao , Shen Zhi Qiang , Yang Ren Hua , Tang Chuan Jing , Bai Lin Ying , He Bo , Chen Peng , Yao Rong Cheng . Effects of Corilagin on Expression of CRP in OX-LDL Injured Macrophage Cells. Journal of Kunming Medical University, 2017, 38(08): 1-5.
[11]	Yang Rong , Yang Ying . . Journal of Kunming Medical University, 2017, 38(05): 44-49.
[12]	Dong Zhong Li . Monocytes/Macrophages Promote Venous Thrombosis Organization and Recanalization：Mechanism and Research Progress. Journal of Kunming Medical University,
[13]	Ge Jia Yun . Effects of FHIT Overexpression Mediated by Slow Virus on the growth of Hepatoma Lines in Vitro. Journal of Kunming Medical University,
[14]	Xie Yuan Run . Experimental Study on The Chemotaxis of Blood Monocyte to Three Kinds of Glioma Cell Lines(U87,U251 and T98G) in Vitro. Journal of Kunming Medical University,
[15]	Li Ding Biao . . Journal of Kunming Medical University,
[16]	Tang Ying . . Journal of Kunming Medical University, 2015, 36(08): 1-1.
[17]	Tai Wen Lin . . Journal of Kunming Medical University,
[18]	Bian Hai Xia . . Journal of Kunming Medical University,
[19]	Shi Zhao Kun . . Journal of Kunming Medical University,
[20]	. Flow Cytomertry Helps to Screen and Purify the HIF-1α gene Silenced Transfected Hepatoma Carcinoma Cells. Journal of Kunming Medical University,