Effects of HSV-1 Mutant Strain M6 on Macrophage-Mediated Immune Response after Infecting Human Bronchial Epithelial Cells

Zhenxiao ZHANG; Jingjing ZHANG; Yun LIAO; Dandan LI; Heng LI; Longding LIU

doi:10.12259/j.issn.2095-610X.S20240702

Volume 45 Issue 7

Jul. 2024

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Zhenxiao ZHANG, Jingjing ZHANG, Yun LIAO, Dandan LI, Heng LI, Longding LIU. Effects of HSV-1 Mutant Strain M6 on Macrophage-Mediated Immune Response after Infecting Human Bronchial Epithelial Cells[J]. Journal of Kunming Medical University, 2024, 45(7): 6-13. doi: 10.12259/j.issn.2095-610X.S20240702

Citation:

Zhenxiao ZHANG, Jingjing ZHANG, Yun LIAO, Dandan LI, Heng LI, Longding LIU. Effects of HSV-1 Mutant Strain M6 on Macrophage-Mediated Immune Response after Infecting Human Bronchial Epithelial Cells[J]. Journal of Kunming Medical University, 2024, 45(7): 6-13. doi: 10.12259/j.issn.2095-610X.S20240702

Citation:

Zhenxiao ZHANG, Jingjing ZHANG, Yun LIAO, Dandan LI, Heng LI, Longding LIU. Effects of HSV-1 Mutant Strain M6 on Macrophage-Mediated Immune Response after Infecting Human Bronchial Epithelial Cells[J]. Journal of Kunming Medical University, 2024, 45(7): 6-13. doi: 10.12259/j.issn.2095-610X.S20240702

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Effects of HSV-1 Mutant Strain M6 on Macrophage-Mediated Immune Response after Infecting Human Bronchial Epithelial Cells

doi: 10.12259/j.issn.2095-610X.S20240702

Zhenxiao ZHANG^{1, 2},
Jingjing ZHANG¹,
Yun LIAO^{1, 2},
Dandan LI^{1, 2},
Heng LI^{1, 2
,
,},
Longding LIU^{1, 2
,
,}

1.
Institute of Medical Biology，Chinese Academy of Medical Sciences & Peking Union Medical College，Kunming Yunnan 650118
2.
Key Laboratory of Systemic Innovative Research on Virus Vaccine，Chinese Academy of Medical Sciences，Kunming Yunnan 650118，China

Received Date: 2024-03-27
Available Online: 2024-06-14
Publish Date: 2024-07-25

Abstract

Abstract

Objective To explore the impact of herpes simplex virus type 1 （HSV-1） mutant strain M6 on macrophage-mediated immune responses after infecting human bronchial epithelial cells （16HBE cells）. Methods HSV-1 infection of 16HBE cells was conducted to assess cytokine alterations in the culture medium; Macrophages were co-cultured with supernatants from HSV-1-infected 16HBE cells and subsequently reintroduced into mice via tail vein infusion. Cytokine expression levels in mouse lymph nodes, changes in spleen T cell proportions, mouse neutralizing antibody levels, and specific T cell responses were measured on days 1, 3, 7, 28, 56, and 90. Results Following infection of 16HBE cells with the HSV-1 mutant strain, cytokine expression in the supernatant, associated with macrophage recruitment and activation, was elevated, albeit slightly lower than that observed in the wild-type strain group. Following the tail vein infusion experiment, the mutant strain group exhibited temporal changes in the proportions of inflammatory factors, chemokines, and T cells within the mouse lymph nodes, resulting in weaker humoral immunity compared to the wild-type strain group, yet eliciting stronger specific T cell responses, with only a few parameters showing significant differences from the wild-type strain group （P < 0.05）. Conclusion 16HBE cells infected with the HSV-1 mutant strain M6 can release cytokines that recruit and activate macrophages, allowing macrophages to carry specific activation information of the HSV-1 mutant strain, activating the host's immune system and inducing the host's Humoral immunity and cellular immunity.
- Herpes simplex virus 1,
- Human bronchial epithelial cells,
- Macrophages,
- Mutant strain

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References(20)

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