Changes of Genebody Methylation in Lingual Carcinogenesis

Hua LIU; Wanyuan YUE; Shuai SHAO; Jiaping SUN; Ying YANG; Xiaoming DAI

doi:10.12259/j.issn.2095-610X.S20240704

Volume 45 Issue 7

Jul. 2024

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Hua LIU, Wanyuan YUE, Shuai SHAO, Jiaping SUN, Ying YANG, Xiaoming DAI. Changes of Genebody Methylation in Lingual Carcinogenesis[J]. Journal of Kunming Medical University, 2024, 45(7): 23-29. doi: 10.12259/j.issn.2095-610X.S20240704

Citation:

Hua LIU, Wanyuan YUE, Shuai SHAO, Jiaping SUN, Ying YANG, Xiaoming DAI. Changes of Genebody Methylation in Lingual Carcinogenesis[J]. Journal of Kunming Medical University, 2024, 45(7): 23-29. doi: 10.12259/j.issn.2095-610X.S20240704

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Changes of Genebody Methylation in Lingual Carcinogenesis

doi: 10.12259/j.issn.2095-610X.S20240704

1.
Dept. of Oral and Maxillofacial Surgery，The Affiliated Hospital of Yunnan University，kunming Yunnan 650021
2.
Dept. of Pathology
3.
Maxillofacial Service of the Department of Plastic Surgery，The 1st Affiliated Hospital of Kunming Medical University，kunming Yunnan 650031，China

Received Date: 2023-12-22
Available Online: 2024-06-15
Publish Date: 2024-07-25

Abstract

Abstract

Objective To evaluate differentially expressed genes with correlation of gene body methylation . Methods The lingual carcinogenesis was induced through the delivery of drinking water of 4-nitroquinoline-1-oxide（4NQO） with a density of 50 mg/L. The lingual mucosae specimens were harvested at 0, 12, 28 weeks, which represented normal, pre-cancer, and cancer individually. The microarray and methylated DNA immunoprecipitation sequencing （MeDIP-Seq） were used for the evaluation of gene expression and global DNA methylation in these specimens. The mRNA expression of Ubiquitin carboxyl-terminal hydrolase CYLD was tested with qRT-PCR in human normal and dysplasia mucosa. Results There were no statistical differences in gene body methylation among normal（C）, pre-cancer（M）, and cancer（E）（mean±sofMeDIP-score were 5.58±14.80, 5.79±13.35, 5.83±17.25 individually, H = 100.75, P > 0.05）There presented 145 differentially expressed genes with positive correlation to gene body methylation changes.The expression of CYLD mRNA was decreased in human dysplasia compared to normal mucosa with downregulation of gene body methylation. Conclusions There are many differentially expressed genes with a positive correlation to gene body methylations. The expression of CYLD is downregulated with the downregulation of gene body methylation in earlier stage during tongue carcinogenesis.
- Tongue cancer,
- Animal model,
- Methylation,
- Gene body,
- Differentially expressed gene

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References(20)

References

[1]	Siegel R L,Giaquinto A N,Jemal A. Cancer statistics,2024[J]. CA Cancer J Clin,2024,74(1):12-49. doi: 10.3322/caac.21820
[2]	代晓明,刘华,左志斌,等. 4-硝基喹啉-1-氧化物诱导C57BL/6小鼠舌黏膜癌变的研究[J]. 华西口腔医学杂志,2015,33(4):357-360. doi: 10.7518/hxkq.2015.04.007
[3]	Lai Z L,Tsou Y A,Fan S R,et al. Methylation-associated gene silencing of RARB in areca carcinogens induced mouse oral squamous cell carcinoma[J]. Biomed Res Int,2014,2014:378358.
[4]	Human D. Declaration of helsinki[J]. Lancet,2001,357(9251):236.
[5]	Kundaje A,Meuleman W,Ernst J,et al. Integrative analysis of 111 reference human epigenomes[J]. Nature,2015,518(7539):317-330. doi: 10.1038/nature14248
[6]	Lorincz M C,Dickerson D R,Schmitt M,et al. Intragenic DNA methylation alters chromatin structure and elongation efficiency in mammalian cells[J]. Nat Struct Mol Biol,2004,11(11):1068-1075. doi: 10.1038/nsmb840
[7]	Naftelberg S,Schor I E,Ast G,et al. Regulation of alternative splicing through coupling with transcription and chromatin structure[J]. Annu Rev Biochem,2015,84:165-198. doi: 10.1146/annurev-biochem-060614-034242
[8]	Bell J S K,Vertino P M. Orphan CpG islands define a novel class of highly active enhancers[J]. Epigenetics,2017,12(6):449-464. doi: 10.1080/15592294.2017.1297910
[9]	Huang K K,Ramnarayanan K,Zhu F,et al. Genomic and epigenomic profiling of high-risk intestinal metaplasia reveals molecular determinants of progression to gastric cancer[J]. Cancer Cell,2018,33(1):137-150e5. doi: 10.1016/j.ccell.2017.11.018
[10]	Zhang F F,Zhang X,Zhang H K,et al. Pan-precancer and cancer DNA methylation profiles revealed significant tissue specificity of interrupted biological processes in tumorigenesis[J]. Epigenetics,2023,18(1):2231222. doi: 10.1080/15592294.2023.2231222
[11]	Hernández H G,Aranzazu-Moya G C,Pinzón-Reyes E H. Aberrant AHRR,ADAMTS2 and FAM184 DNA methylation: Candidate biomarkers in the oral rinse of heavy smokers[J]. Biomedicines,2023,1(7):1797.
[12]	Li X,Li Z,Gao Q,et al. Correlation of DNA methylation of DNMT3A and TET2 with oral squamous cell carcinoma[J]. Discov Oncol,2024,15(1):15. doi: 10.1007/s12672-024-00866-9
[13]	Gabusi A,Gissi D B,Grillini S,et al. Shared epigenetic alterations between oral cancer and periodontitis: A preliminary study[J]. Oral Dis,2023,9(5):2052-2060.
[14]	Aghiorghiesei O,Irimie AI,Braicu C,et al. Epigenetic methylation changes: Implication as biomarkers in oral and maxillofacial area cancers[J]. Med Pharm Rep,2023,96(3):310-317.
[15]	Okoturo E,Green D,Clarke K,et al. Whole genome DNA methylation and mutational profiles identify novel changes in proliferative verrucous leukoplakia[J]. Oral Surg Oral Med Oral Pathol Oral Radiol,2023,135(6):893-903. doi: 10.1016/j.oooo.2023.03.004
[16]	Rivera-Peña B,Folawiyo O,Turaga N,et al. Promoter DNA methylation patterns in oral,laryngeal and oropharyngeal anatomical regions are associated with tumor differentiation,nodal involvement and survival[J]. Oncol Lett,2024,27(3):89. doi: 10.3892/ol.2024.14223
[17]	Dean K A,von Ahsen O,Görlich D,et al. Signal recognition particle protein 19 is imported into the nucleus by importin 8 (RanBP8) and transportin[J]. J Cell Sci,2001,114(Pt 19): 3479-3485.
[18]	Giussani P,Maceyka M,Le Stunff H,et al. Sphingosine-1-phosphate phosphohydrolase regulates endoplasmic reticulum-to-golgi trafficking of ceramide[J]. Mol Cell Biol,2006,26(13):5055-5069. doi: 10.1128/MCB.02107-05
[19]	Lv X,Yu X. Signatures and prognostic values of related immune targets in tongue cancer[J]. Front Surg,2023,9:952389. doi: 10.3389/fsurg.2022.952389
[20]	Thakore V P,Patel K D,Vora H H,et al. Up-regulation of extracellular-matrix and inflammation related genes in oral squamous cell carcinoma[J]. Arch Oral Biol,2024,161:105925. doi: 10.1016/j.archoralbio.2024.105925

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