Changes of Genebody Methylation in Lingual Carcinogenesis

Hua LIU; Wanyuan YUE; Shuai SHAO; Jiaping SUN; Ying YANG; Xiaoming DAI

doi:10.12259/j.issn.2095-610X.S20240704

Volume 45 Issue 7

Jul. 2024

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Hua LIU, Wanyuan YUE, Shuai SHAO, Jiaping SUN, Ying YANG, Xiaoming DAI. Changes of Genebody Methylation in Lingual Carcinogenesis[J]. Journal of Kunming Medical University, 2024, 45(7): 23-29. doi: 10.12259/j.issn.2095-610X.S20240704

Citation:

Hua LIU, Wanyuan YUE, Shuai SHAO, Jiaping SUN, Ying YANG, Xiaoming DAI. Changes of Genebody Methylation in Lingual Carcinogenesis[J]. Journal of Kunming Medical University, 2024, 45(7): 23-29. doi: 10.12259/j.issn.2095-610X.S20240704

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Changes of Genebody Methylation in Lingual Carcinogenesis

doi: 10.12259/j.issn.2095-610X.S20240704

1.
Dept. of Oral and Maxillofacial Surgery，The Affiliated Hospital of Yunnan University，kunming Yunnan 650021
2.
Dept. of Pathology
3.
Maxillofacial Service of the Department of Plastic Surgery，The 1st Affiliated Hospital of Kunming Medical University，kunming Yunnan 650031，China

Received Date: 2023-12-22
Available Online: 2024-06-15
Publish Date: 2024-07-25

Abstract

Abstract

Objective To evaluate differentially expressed genes with correlation of gene body methylation . Methods The lingual carcinogenesis was induced through the delivery of drinking water of 4-nitroquinoline-1-oxide（4NQO） with a density of 50 mg/L. The lingual mucosae specimens were harvested at 0, 12, 28 weeks, which represented normal, pre-cancer, and cancer individually. The microarray and methylated DNA immunoprecipitation sequencing （MeDIP-Seq） were used for the evaluation of gene expression and global DNA methylation in these specimens. The mRNA expression of Ubiquitin carboxyl-terminal hydrolase CYLD was tested with qRT-PCR in human normal and dysplasia mucosa. Results There were no statistical differences in gene body methylation among normal（C）, pre-cancer（M）, and cancer（E）（mean±sofMeDIP-score were 5.58±14.80, 5.79±13.35, 5.83±17.25 individually, H = 100.75, P > 0.05）There presented 145 differentially expressed genes with positive correlation to gene body methylation changes.The expression of CYLD mRNA was decreased in human dysplasia compared to normal mucosa with downregulation of gene body methylation. Conclusions There are many differentially expressed genes with a positive correlation to gene body methylations. The expression of CYLD is downregulated with the downregulation of gene body methylation in earlier stage during tongue carcinogenesis.
- Tongue cancer,
- Animal model,
- Methylation,
- Gene body,
- Differentially expressed gene

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References(20)

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