Polymorphism Investigation of 23 Autosome STR Loci in the Microreader<sup>TM</sup> 23HS Plex ID System in Han Population in Northern China

Yan SHI; Jian JIANG; Chong CHEN; Jingjing ZHANG; Li JIA; Zhixiao GAO; Huifen LI; Jiangwei YAN; He REN

doi:10.12259/j.issn.2095-610X.S20240723

Volume 45 Issue 7

Jul. 2024

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Article Navigation > Journal of Kunming Medical University > 2024 > 45(7): 154-159

Yan SHI, Jian JIANG, Chong CHEN, Jingjing ZHANG, Li JIA, Zhixiao GAO, Huifen LI, Jiangwei YAN, He REN. Polymorphism Investigation of 23 Autosome STR Loci in the MicroreaderTM 23HS Plex ID System in Han Population in Northern China[J]. Journal of Kunming Medical University, 2024, 45(7): 154-159. doi: 10.12259/j.issn.2095-610X.S20240723

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Yan SHI, Jian JIANG, Chong CHEN, Jingjing ZHANG, Li JIA, Zhixiao GAO, Huifen LI, Jiangwei YAN, He REN. Polymorphism Investigation of 23 Autosome STR Loci in the Microreader^TM 23HS Plex ID System in Han Population in Northern China[J]. Journal of Kunming Medical University, 2024, 45(7): 154-159. doi: 10.12259/j.issn.2095-610X.S20240723

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Polymorphism Investigation of 23 Autosome STR Loci in the Microreader^TM 23HS Plex ID System in Han Population in Northern China

doi: 10.12259/j.issn.2095-610X.S20240723

1.
Beijing Tongda Shoucheng Institute of Forensic Science，Beijing 100192
2.
Fujian Nanfang Judicial Expertise Center，Fuzhou Fujian 350000
3.
Beijing Huayan Judicial Authentication Institute，Beijing 100192
4.
Suzhou Microread Gene Technology Co.，LTD，Suzhou Jiangsu 215000
5.
College of Medicine and Forensics，Shanxi Medical University，Jinzhong Shanxi 030600
6.
Dept. of Criminal Science and Technology，Beijing Police College，Beijing 102202，China

Received Date: 2024-03-02
Available Online: 2024-06-14
Publish Date: 2024-07-25

Abstract

Abstract

Objective To obtain the distribution of allele frequency and population genetic data of 23 autosomal STR loci contained in the Microreader^TM 23HS Plex ID System kit in the Han population of northern China, and explore its application value in forensic medicine. Methods The Microreader^TM 23HS Plex ID System kit was used to detect the DNA of 548 unrelated samples which from the Han population in northern China. Then, the allele frequencies, heterozygosity（H） in the samples, polymorphism information content（PIC）, power of discrimination（DP）, and probability of paternity exclusion（PE） were calculated and Hardy-Weinberg equilibrium was tested on each locus based on the genotyping data of 548 samples we collected. The cumulative individual identification ability （CDP） and cumulative non-paternity exclusion rate （CPE）of the Microreader^TM 23HS Plex ID System were also calculated. Results A total of 260 alleles were detected in 548 unrelated samples from 23 STR loci which conformed to Hardy-Weinberg equilibrium. And the alleles frequencies ranged from 0.0009 to 0.5902, H ranged from 0.611 to 0.885, PIC ranged from 0.577 to 0.864, DP ranged from 0.815 to 0.973 （mean DP was 0.922） and PE ranged from 0.089 to 0.406. At the same time, the CDP of Microreader^TM 23HS Plex ID System was 1-3.663×10^-27 and CPE was 1-2.668×10^-16. Conclusion The 23 loci of Microreader^TM 23HS Plex ID System have a good polymorphism in the north Chinese Han population, and have high application value in population genetics research, forensic personal identification, paternity testing and complex kinship identification.
- Forensic genetics,
- Genetic polymorphism,
- STR loci

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