Disinfecting Effect of Three Disinfectants on the Ultrasonic Scaling Unit Waterline

Yang LYU; Guiding LI; Shaowen LU; Binbing YU; Qiuju ZHAI; Yi PENG

doi:10.12259/j.issn.2095-610X.S20240928

Volume 45 Issue 9

Sep. 2024

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Article Navigation > Journal of Kunming Medical University > 2024 > 45(9): 180-188

Yang LYU, Guiding LI, Shaowen LU, Binbing YU, Qiuju ZHAI, Yi PENG. Disinfecting Effect of Three Disinfectants on the Ultrasonic Scaling Unit Waterline[J]. Journal of Kunming Medical University, 2024, 45(9): 180-188. doi: 10.12259/j.issn.2095-610X.S20240928

Citation:

Yang LYU, Guiding LI, Shaowen LU, Binbing YU, Qiuju ZHAI, Yi PENG. Disinfecting Effect of Three Disinfectants on the Ultrasonic Scaling Unit Waterline[J]. Journal of Kunming Medical University, 2024, 45(9): 180-188. doi: 10.12259/j.issn.2095-610X.S20240928

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Disinfecting Effect of Three Disinfectants on the Ultrasonic Scaling Unit Waterline

doi: 10.12259/j.issn.2095-610X.S20240928

Yang LYU^{1, 2},
Guiding LI²,
Shaowen LU¹,
Binbing YU³,
Qiuju ZHAI^{1
,
,},
Yi PENG^{1
,
,}

1.
Dept. of Periodontology，Kunming Medical University School and Hospital of Stomatology，Kunming Yunnan 650106
2.
Yunnan Key Laboratory of Stomatology，Kunming Yunnan 650500
3.
Dept. of Anorectum，Kunming Municipal Hospital of Traditional Chinese Medicine，Kunming Yunnan 650106，China

Received Date: 2024-05-14
Available Online: 2024-09-04
Publish Date: 2024-09-25

Abstract

Abstract

Objective To assess the efficacy of three disinfectants in disinfecting the ultrasonic scaling unit waterline. Methods Twelve periodontal ultrasonic scalers were randomly divided into four groups based on the disinfection protocol: distilled water （A group）, 3% hydrogen peroxide （B group）, 500 mg/L chlorine disinfectant （C group） and 5% povidone-iodine （D group）. Each scaler was used for daily periodontal treatment and worked effectively for 6 hours per day. Water samples were collected at baseline, after disinfection, and 1-7 days post-disinfection. Scanning electron microscopy （SEM） was used to observe biofilm formation. Culturable bacteria were counted, isolated and purified, then identified through 16S rRNA gene sequencing. Sequences of 16S rRNA gene amplicons were clustered by operational taxonomic units （OTUs）. Results Baseline colony counts exceeded 100 CFU/mL in all groups. However, after disinfection, the colony counts decreased significantly in all groups （P < 0.05）. The colony counts remained below 100 CFU/mL within 3 d after disinfection with 3% H₂O₂, within 6 days after disinfection with 500 mg/L chlorine-containing disinfectant, and within 4 d after disinfection with 5% povidone-iodine （P < 0.05）.Pathogens such as Stenotrophomonas maltophilia were detected in the ultrasonic scaler unit water circuit. SEM showed thinner biofilm thickness in the 3% H₂O₂ and 5% povidone-iodine groups compared to the control group （P < 0.05）. Conclusions Bacterial contamination is present in the ultrasonic scaling unit waterline, posing a potential infection risk to periodontal staff and patients. In the context of increasingly severe nosocomial infections, appropriate disinfection protocols should be developed based on clinical realities.
- Ultrasonic scaler,
- Dental unit waterline,
- Plaque biofilms,
- Hydrogen peroxide,
- Sodium hypochlorite,
- Povidone iodine

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References(41)

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