The Mechanism of miR-23 Regulating PI3K/AKT/mTOR Pathway to Improve Myocardial Angiogenesis in Hypertensive Heart Failure Rats

Haixing ZHANG; Jingyun ZHANG; Dandan XU; Lu CAO; Jingjing LI

doi:10.12259/j.issn.2095-610X.S20251105

Volume 46 Issue 11

Nov. 2025

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Article Navigation > Journal of Kunming Medical University > 2025 > 46(11): 35-42

Haixing ZHANG, Jingyun ZHANG, Dandan XU, Lu CAO, Jingjing LI. The Mechanism of miR-23 Regulating PI3K/AKT/mTOR Pathway to Improve Myocardial Angiogenesis in Hypertensive Heart Failure Rats[J]. Journal of Kunming Medical University, 2025, 46(11): 35-42. doi: 10.12259/j.issn.2095-610X.S20251105

Citation:

Haixing ZHANG, Jingyun ZHANG, Dandan XU, Lu CAO, Jingjing LI. The Mechanism of miR-23 Regulating PI3K/AKT/mTOR Pathway to Improve Myocardial Angiogenesis in Hypertensive Heart Failure Rats[J]. Journal of Kunming Medical University, 2025, 46(11): 35-42. doi: 10.12259/j.issn.2095-610X.S20251105

Citation:

Haixing ZHANG, Jingyun ZHANG, Dandan XU, Lu CAO, Jingjing LI. The Mechanism of miR-23 Regulating PI3K/AKT/mTOR Pathway to Improve Myocardial Angiogenesis in Hypertensive Heart Failure Rats[J]. Journal of Kunming Medical University, 2025, 46(11): 35-42. doi: 10.12259/j.issn.2095-610X.S20251105

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The Mechanism of miR-23 Regulating PI3K/AKT/mTOR Pathway to Improve Myocardial Angiogenesis in Hypertensive Heart Failure Rats

doi: 10.12259/j.issn.2095-610X.S20251105

1.
Department of Cardiology，Dingzhou People’s Hospital，Baoding Hebei 073000
2.
Department of Internal Medicine，Yongqing County People’s Hospital，Langfang Hebei 065600
3.
Department of Cardiovascular Medicine，People’s Hospital of Yanshan County，Cangzhou Hebei 061000，China

Received Date: 2025-04-10
Available Online: 2025-11-06
Publish Date: 2025-11-25

Abstract

Abstract

Objective To investigate the mechanisms by which miR-23 regulates the PI3K/AKT/mTOR signaling pathway to influence myocardial remodeling, fibrosis, and angiogenesis in hypertensive heart failure （HF） rats. Methods Forty rats were randomly divided into four groups （n = 10 per group）: a control group, a model group, an antagomir-NC group, and an antagomir-23 group. The HF model was established using a high-salt diet, and intervention was performed via tail vein injection of antagomir-23. Cardiac function parameters, the degree of myocardial fibrosis, cell apoptosis levels, and the expression of angiogenesis-related proteins including CD31, VEGF, and bFGF were measured in each group. Concurrently, the activity of the PI3K/AKT/mTOR signaling pathway was assessed. A dual-luciferase reporter assay was conducted to confirm that miR-23 targets PI3K. Results Inhibition of miR-23 significantly improved cardiac function in hypertensive HF rats, reduced myocardial fibrosis and apoptosis, and enhanced the expression of CD31, VEGF, bFGF, and activated the PI3K/AKT/mTOR signaling pathway in hypertensive HF rats （all P < 0.05）. The dual-luciferase assay confirmed that miR-23 negatively regulates PI3K expression. Conclusion Inhibition of miR-23 can activate the PI3K/AKT/mTOR signaling pathway, promote angiogenesis, reduce myocardial damage, thereby delaying the progression of hypertensive heart failure.
- Hypertension,
- Heart failure,
- miR-23,
- Myocardial remodeling,
- Fibrosis,
- Angiogenesis

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References(25)

References

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