Effects of MiR-126 on Myocardial Remodeling and Macrophage Polarization after Acute Myocardial Infarction

Jiaojiao HAN; Liwei AN; Yangyang XIE; Bin WANG; Jin WANG; Lijun ZHANG

doi:10.12259/j.issn.2095-610X.S20251106

Volume 46 Issue 11

Nov. 2025

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Jiaojiao HAN, Liwei AN, Yangyang XIE, Bin WANG, Jin WANG, Lijun ZHANG. Effects of MiR-126 on Myocardial Remodeling and Macrophage Polarization after Acute Myocardial Infarction[J]. Journal of Kunming Medical University, 2025, 46(11): 43-49. doi: 10.12259/j.issn.2095-610X.S20251106

Citation:

Jiaojiao HAN, Liwei AN, Yangyang XIE, Bin WANG, Jin WANG, Lijun ZHANG. Effects of MiR-126 on Myocardial Remodeling and Macrophage Polarization after Acute Myocardial Infarction[J]. Journal of Kunming Medical University, 2025, 46(11): 43-49. doi: 10.12259/j.issn.2095-610X.S20251106

Citation:

Jiaojiao HAN, Liwei AN, Yangyang XIE, Bin WANG, Jin WANG, Lijun ZHANG. Effects of MiR-126 on Myocardial Remodeling and Macrophage Polarization after Acute Myocardial Infarction[J]. Journal of Kunming Medical University, 2025, 46(11): 43-49. doi: 10.12259/j.issn.2095-610X.S20251106

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Effects of MiR-126 on Myocardial Remodeling and Macrophage Polarization after Acute Myocardial Infarction

doi: 10.12259/j.issn.2095-610X.S20251106

1.
Department of Cardiology
2.
Department of Hematology，Dingzhou People’s Hospital，Dingzhou Hebei 073000
3.
Department of Internal Medicine
4.
Emergency Department，Yongqing County People’s Hospital，Hebei Langfang 065600
5.
Department of Hematology，the Third Hospital of Shijiazhuang，Shijiazhuang Hebei 050000，China

Received Date: 2025-04-09
Available Online: 2025-11-24
Publish Date: 2025-11-25

Abstract

Abstract

Objective To investigate the effects of miR-126 on myocardial remodeling and macrophage polarization after acute myocardial infarction （AMI）. Methods （1） Twenty-one rats were divided into a sham operation group and an AMI group. The AMI group was further divided into postoperative days 1, 3, 5, 7, 9, and 11 days （AMI-1, AMI-3, AMI-5, AMI-7, AMI-9, AMI-11）, with 3 rats in each group, and postoperative day 3 was selected for subsequent study. （2） Thirty rats were randomly divided into three groups: sham operation group, AMI group and miR-126 overexpression group, with 10 rats in each group. RT-qPCR was used to detect the expression levels of miR-126 mRNA. 2, 3, 5-Triphenyltetrazole chloride （TTC） staining was used to detect the infarct size. Myocardial fibrosis was detected by Masson staining. The cross-sectional area of the myocardium was determined by H&E staining. Immunofluorescence staining was used to detect the protein levels of CD86 and CD206 in myocardial tissue. Western blotting was used to detect the protein levels of CD86 and CD206 in myocardial tissue. The levels of tumor necrosis factor-α （TNF-α）, interleukin-1β （IL-1β） and IL-6 in serum were detected by ELISA kit. Results Compared with the sham operation group, the expression level of miR-126 mRNA in myocardial tissue of rats in the AMI group was significantly decreased on postoperative day 1 （P < 0.05）, reached the lowest level on postoperative day 3. And then although it rose to a certain extent, it remained lower than that of the sham operation group on postoperative day 11 （P < 0.05）, and postoperative day 3 was selected for further study. Compared with the sham operation group, the expression of miR-126 mRNA in myocardial tissue of the AMI group was decreased （P < 0.05）, and the myocardial fibrosis, infarct size and myocardial cross-sectional area were increased （P < 0.05）. The expression of CD86 protein in myocardial tissue was increased and the expression of CD206 protein was decreased （P < 0.05）. The serum levels of TNF-α, IL-6 and IL-1β were increased （P < 0.05）. Compared with the AMI group, the rats in the miR-126 overexpression group had a significant increase in the mRNA expression of miR-126 in myocardial tissue （P < 0.05）, a significant reduction in myocardial fibrosis, infarct size, and myocardial cross-sectional area （P < 0.05）, a significant reduction in the expression of CD86 protein in myocardial tissue, and a significant increase in the expression of CD206 protein （P < 0.05）. The serum levels of TNF-α, IL-6 and IL-1β were significantly decreased （P < 0.05）. Conclusion Over-expression of miR-126 can improve myocardial remodeling and promote M2 macrophage polarization in AMI rats.
- Acute myocardial infarction,
- miR-126,
- Polarization of macrophages,
- Myocardial remodeling

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References(20)

References

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