Construction and in Vitro Expression of Cleft Palate Relevant Gene TTF-2 Transgenic Mice Vector

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Construction and in Vitro Expression of Cleft Palate Relevant Gene TTF-2 Transgenic Mice Vector[J]. Journal of Kunming Medical University, 2011, 32(07).

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Construction and in Vitro Expression of Cleft Palate Relevant Gene TTF-2 Transgenic Mice Vector[J]. Journal of Kunming Medical University, 2011, 32(07).

Construction and in Vitro Expression of Cleft Palate Relevant Gene TTF-2 Transgenic Mice Vector[J]. Journal of Kunming Medical University, 2011, 32(07).

Citation:

Construction and in Vitro Expression of Cleft Palate Relevant Gene TTF-2 Transgenic Mice Vector[J]. Journal of Kunming Medical University, 2011, 32(07).

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Construction and in Vitro Expression of Cleft Palate Relevant Gene TTF-2 Transgenic Mice Vector

Abstract

Abstract

Objective To amplify and clone the C57BL/6J mouse titf-2 gene and express its protein in the mouse marrow mesenchymal cell.Methods The DNA encoding the C57BL/6J mouse titf-2 gene was amplified by PCR from the mouse liver tissue.The titf-2 gene was inserted into plasmid pMD18-T.After sequencing,The titf-2 gene was cloned into eukaryotic expression vector pBROAD3-mcs to construct the recombinant expression vector pBROAD3-titf-2 and it was used to transfect the mouse marrow mesenchymal cell.The expression prod...
- TTF-2,
- Cloning,
- Western bloting

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