Effects of Liangxuehuoxue Drugs on Hyperoxia Induced Retinal Neovascular HIF-1 and NF-кB Expression in Mice

［Abstract］Objective　To study the intervention of liangxuehuoxue drugs on retinal neovascularization and the mechanism of action．Methods　Retinal neovascularization model was established in young C57BL/6 mice by hyperoxia induction．Mice were divided randomly into 4 groups：normal control group，model group， liangxuehuoxue drugs high dose group and low dose group. The number of nucleus of endothelial cells which broke through the endangium in retinal section， and the expression of HIF-1 and NF-κB in retina was detected by immunohistochemistry．Results　Comapred with normal control，after hyperoxia induction，the number of nucleus of endothelial cells significantly increased， and the expression of HIF-1 and NF-κB was upregulated， and the difference was statistically significant（P＜0.05），suggesting the retinal neovascularization model was established successfully. Compared with model group， in liangxuehuoxue drugs groups，the number of nucleus of endothelial cells significantly decreased， and the expression of HIF-1 and NF-κB was downregulated，and the difference was statistically significant（P＜0.05）．Conclusion　Liangxuehuoxue can inhibit the retinal neovascularization，and the mechanism may be related to downregulating the expression of HIF-1 and NF-κB related to the angiogenesis.