Toxicological Study on the Anticancer Bioactive Fraction AMH-T of Lichen

He Yun Fa; He Xiao Qiong; Jiang Zhong Yang; Wang Jing; Hu Song Shan; Shui Jing Lin

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He Yun Fa , He Xiao Qiong , Jiang Zhong Yang , Wang Jing , Hu Song Shan , Shui Jing Lin . Toxicological Study on the Anticancer Bioactive Fraction AMH-T of Lichen[J]. Journal of Kunming Medical University, 2016, 37(11): 1-4.

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He Yun Fa , He Xiao Qiong , Jiang Zhong Yang , Wang Jing , Hu Song Shan , Shui Jing Lin . Toxicological Study on the Anticancer Bioactive Fraction AMH-T of Lichen[J]. Journal of Kunming Medical University, 2016, 37(11): 1-4.

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Toxicological Study on the Anticancer Bioactive Fraction AMH-T of Lichen

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基金：国家自然科学基金资助项目(30960437); 云南省科技计划项目(2009FXL001); 云南省科技厅联合专项重点项目(2012FB001);

Received Date: 2016-05-03

Abstract

Abstract

Objective To investigate the effects of anticancer bioactive fraction AMH-T of lichen on blood routine, organ coefficient and organ morphology by carrying out short-term repeated dose toxicity test in rat so as to provide evidence for the development of anticancer drugs. Methods The nude mice were randomly divided into 5groups: DDP group, DMSO group, and three AMH-T groups with the dosage of 50mg/kg, 100mg/kg, and 200 mg/kg respectively. The weights of the mice were recorded every four days. At the end of the experiment, automatic biochemical analyzer and blood cell analyzer were applied to detect the serum biochemical indicators and blood routine indexes. The mice were dissected to observe the pathological changes in main organs. Heart, liver, spleen,kidney and testicle were weighed for organ coefficient calculation. Results In short-term repeated dose toxicity test,AMH-T significantly increased blood ALT and AST levels(P<0.01) and significant change was found in other blood biochemical indexes and blood routine indexes. AMH-T had no obvious effect on weight,development of heart, liver,spleen, kidney and testicle. Conclusion When subcutaneous injection is performed, AMH-T shows hepatotoxicity,but it shows no toxicity on bone marrow hematopoietic function.
- Lichen,
- Anticancer drug,
- Acute toxicity,
- Genotoxicity,
- Toxicology

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References(25)

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