Study of the Expression Differences of FHIT Protein in 90 Hepatocellular Tissues with Tissue Chip Method

［Abstract］Objective　To explore the expressions differences of the FHIT gene in 90 human hepatocellular carcinoma tissues with tissue chip method. Methods 90 cases of patients with primary liver cancer of complete the clinic pathological data and its surgical specimens to keep in the archives with paraffin blocks were collected. FHIT gene expressional differences in the above specimens were determined by IHC-tissue chip. Results　Whether qualitative or quantitative analysis，IHC test results showed that there was a significant difference in expressions of FHIT protein between hepatocellular carcinoma tissue and para-carcinoma tissue. The positive expression of FHIT protein in para-carcinoma tissue was significantly higher than hepatocellular carcinoma tissue（P＜0.05）. Conclusions　FHIT gene is a cancer suppressor gene and poor expression of FHIT gene is correlated with malignant differentiation of liver cancer. Immunohistochemical method is helpful to assist the early diagnosis and make a reasonable appraisal of their prognosis.