Optimizing Preparation of Biotin-microbubbles of Ultrasound

Yang Yang

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Yang Yang . Optimizing Preparation of Biotin-microbubbles of Ultrasound[J]. Journal of Kunming Medical University, 2016, 37(01).

Citation:

Yang Yang . Optimizing Preparation of Biotin-microbubbles of Ultrasound[J]. Journal of Kunming Medical University, 2016, 37(01).

Yang Yang . Optimizing Preparation of Biotin-microbubbles of Ultrasound[J]. Journal of Kunming Medical University, 2016, 37(01).

Citation:

Yang Yang . Optimizing Preparation of Biotin-microbubbles of Ultrasound[J]. Journal of Kunming Medical University, 2016, 37(01).

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Optimizing Preparation of Biotin-microbubbles of Ultrasound

Yang Yang

(1)Dept. of Ultrasound,The 1st Affiliated Hospital of Kunming Medical University,Ultrasonic research center of Yunnan Province,Kunming Yunnan 650031;2)Dept of Ultrasound,Xinqiao Hospital,Third Military Medical University,Chongqing 400037;3)Yunnan Institute For Drug Abuse Kunming Yunnan 650228,China)

Abstract

Abstract

［Abstract］Objective　To optimically prepare for biotin-microbubbles with small diameter and good stability，and evaluate the physical and chemical properties，and the effect of contrast．Methods　We prepare lipid vesicles with different proportion for the group A-E.The physical and chemical properties of each group were evaluated by particle size analyzer and Malvern．We evaluated the effect of different solvents on the stability of the group1-3，observed the effect of each group by ultrasonic diagnostic apparatus．Results　There were significant differences between groups A，Cand E:Lipid located the different layers of the vial；With the increasing of DPPC，MPEG200，DPPA，the average size and concentration decreased gradually，the distribution became uniform gradually，and the Zeta decreased gradually.Complex solution made microbubbles with small size，non-rupture，concentration maintain and good stability．Conclusions　DPPC：MPEG200：DPPA：DSPE-PEG2000-Biotin 90:5:5:10 and complex solution can make the best biotin-microbubbles.It is a optimal choice for preparation of target microbubbles.
- ［Key words］Biotin；Avidin；Microbubbles；Preparation；Contrast imaging,

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