Experimental Study of Fiber Glue as A Carrier Compounded with BMP-2 on Repairing Bone Defect

［Abstract］Objective To investigate the effect of the fibrin glue（FG）as a carrier compounded with BMP-2 on repairing bone defect and stimulating early period bone formation，and to provide the experimental foundation for the clinical application. Methods The fibrin glue compounded with growth factor BMP-2 delivery system was prepared with the consistency of BMP-21 μg/mL. Bone defects（8.00 mm length，2.00mm width and 1.00mm depth）were created in 36 rabbits on the bilateral radius and rabbits were divided into three groups. They were treated with three kinds of implantations，including fibrin compounded with BMP-2 in experimental group，fibrin in control group and no material in blank control group. Rabbits were sacrificed at the end of 2 weeks，4 weeks and 8 weeks after operation，respectively. Then specimens were obtained in each time point and bone formation and osteocalcin（OC）were detected by X-ray，histological section and immunohistochemical method. Results The bone forming activity and bone healing speed in experimental group were significantly better than those of the other groups by X-ray and histological section. Immunohistochemical results showed OC contents were highest and there were significant differences among the three groups in the second week（P < 0.01）. In experimental group， the expression amount of OC decreased in local bone and bone callus organization， and the most significant differences among the three groups appeared at the fourth week（P < 0.01）. There was no difference in the expression of OC between the experimental group and the other groups at the eighth week（P > 0.05）. Conclusion The material with fibrin glue as a carrier compounded with BMP-2 could significantly accelerate the new bone forming， regeneration，mineralization and remodeling，shorten the recovery period，which can provide a clinical method for repairing bone defect.