IL-27 Alleviates the Bleomycin-induced Pulmonary Fibrosis through Regulating TGF-β/Smad Signaling Pathway

Lei Wen

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Lei Wen . IL-27 Alleviates the Bleomycin-induced Pulmonary Fibrosis through Regulating TGF-β/Smad Signaling Pathway[J]. Journal of Kunming Medical University, 2016, 37(05).

Citation:

Lei Wen . IL-27 Alleviates the Bleomycin-induced Pulmonary Fibrosis through Regulating TGF-β/Smad Signaling Pathway[J]. Journal of Kunming Medical University, 2016, 37(05).

Lei Wen . IL-27 Alleviates the Bleomycin-induced Pulmonary Fibrosis through Regulating TGF-β/Smad Signaling Pathway[J]. Journal of Kunming Medical University, 2016, 37(05).

Citation:

Lei Wen . IL-27 Alleviates the Bleomycin-induced Pulmonary Fibrosis through Regulating TGF-β/Smad Signaling Pathway[J]. Journal of Kunming Medical University, 2016, 37(05).

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IL-27 Alleviates the Bleomycin-induced Pulmonary Fibrosis through Regulating TGF-β/Smad Signaling Pathway

Lei Wen

(Dept. of Respiratory Medicine,The 2nd Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650101,China)

Abstract

Abstract

［Abstract］Objective To explore whether IL-27 inhibited the pulmonary fibrosis through regulating the expression of TGF-β/Smad signaling pathway in the bleomycin-induced pulmonary fibrosis model. Methods Forty male C57/BL6 mice were randomly divided into normal control group（group A）， bleomycin-induced pulmonary fibrosis group （group B）， bleomycin+IL-27 group（group C）and bleomycin+IL-27 antibody group （group D） with 10 in each. Five mice in each group were sacrificed on days 7 and 28 after with intratracheal bleomycin. TGF-βR1， Smad1 and Smad3 in right lung tissue were measured by Western Blot. Results 1. In the bleomycin-induced pulmonary fibrosis model， the expression of TGF-βR1 was higher on days 7 and 28， which was inhibited by IL-27. 2. The expressions of p-Smad1 and p-Smad3 were highest in group D on days 7 and 28， but were lower in group C on day 7 than those in group B. Conclusion Exogenous IL-27 might alleviate pulmonary fibrosis through inhibiting the related protein phosphorylation in TGF-β/Smad signaling pathway.
- ［Key words］IL-27；Pulmonary fibrosisp；TGF-β/Smad signaling pathway,

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