Effects of Paclitaxel on Gene and Protein Expression of wnt/β-catenin Signaling Pathway in Cholangiocarcinoma Cell Line QBC939

Hou Feng Qiang; Dong Shan Chao; Lei Xi Feng; Zhang Wei

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Hou Feng Qiang , Dong Shan Chao , Lei Xi Feng , Zhang Wei . Effects of Paclitaxel on Gene and Protein Expression of wnt/β-catenin Signaling Pathway in Cholangiocarcinoma Cell Line QBC939[J]. Journal of Kunming Medical University, 2017, 38(04): 10-13.

Citation:

Hou Feng Qiang , Dong Shan Chao , Lei Xi Feng , Zhang Wei . Effects of Paclitaxel on Gene and Protein Expression of wnt/β-catenin Signaling Pathway in Cholangiocarcinoma Cell Line QBC939[J]. Journal of Kunming Medical University, 2017, 38(04): 10-13.

Citation:

Hou Feng Qiang , Dong Shan Chao , Lei Xi Feng , Zhang Wei . Effects of Paclitaxel on Gene and Protein Expression of wnt/β-catenin Signaling Pathway in Cholangiocarcinoma Cell Line QBC939[J]. Journal of Kunming Medical University, 2017, 38(04): 10-13.

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Effects of Paclitaxel on Gene and Protein Expression of wnt/β-catenin Signaling Pathway in Cholangiocarcinoma Cell Line QBC939

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基金：国家自然科学基金资助项目(81560238);

Received Date: 2016-11-14

Abstract

Abstract

Objective To explore the effects of paclitaxel on gene and protein expression of wnt/β-catenin signaling pathway in cholangiocarcinoma cell line QBC939. Methods Western blot was used to detectβ-cateninprotein expression level in 0.1, 1, 10 nmol/L paclitaxel groups and control group for 24 h. q-PCR was used to detect c-jun and p53 mRNA expression level in 10nmol/L paclitaxel group and control group for 24 h.Results Compared with control group, the expression levels of β-catenin protein in paclitaxel groups were decreased, the expression levels of β-catenin protein were significantly reduced when paclitaxel concentration at10 nmol/L(P<0.05). Compared with control group,the expression levels of p53 mRNA were up-regulated and c-jun mRNA were down-regulated in10nmol/L paclitaxel group(P <0.05). Conclusions Paclitaxel may Down-regulate the expression of β-catenin and up-regulate the p53 mRNA, then block the wnt/β-catenin signaling pathway and down-regulate c-jun mRNA in cholangiocarcinoma cell line QBC939.
- Paclitaxel,
- QBC939,
- β-catenin,
- P53,
- C-jun

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References(15)

References

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